plateau

Photonic BioImaging

Platform

Photonic BioImaging

Technical manager

Caroline Clerté

Scientific manager

Christine Doucet

Permanent staff

Emmanuel Margeat, Jean-Bernard Fiche , Antoine Le Gall

The facility comprises 2 types of equipement:

Fluorescence spectroscopy plate readers, dedicated to fluorescence and luminescence characterization of biomacromolecular interactions or folding, enzymatic activity, protein expression… either from gels, solutions dispatched in multi-well plates, cells on petri dishes or multi-well plates. Some readers can do kinetics measurements, with or without injection.
Advanced, custom-built microscopes. Most of this equipment is operated by dedicated research engineers and projects are run on the basis of scientific collaborations.

Equipement

Fluorescence spectroscopy

MARS - BioPhotonics

*Platform*	MARS - BioPhotonics
*Technical manager*	Caroline Clerté
*Scientific manager*	Christine Doucet
*R&D managers*	Jean-Bernard Fiche , Antoine Le Gall
*Permanent staff*	Emmanuel Margeat

The facility offers a large range of equipment and expertise in fluorescence spectroscopy and/or microscopy. The facility is organized around three poles: Fluorescence spectroscopy plate readers. Advanced, custom-built microscopes. This equipment is operated by dedicated research engineers and projects are run on the basis of scientific collaborations. Research & Development division, that studies and proposes new implementations (technical or analytical) to address specific problems Our facility belongs to the FranceBioImaging Infrastructure

Services

Protein characterization	Measuring molecule diffusion	Measuring biomolecular interactions	Measuring protein structural dynamics
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Super-resolved 2D and 3D fluorescence imaging	MARS BioPhotonics R&D division
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Equipement

Fluorescence spectroscopy

Berthold Mithras LB940		CLARIOstar	Cytation 3	Synergy H1
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TECAN Safire2		Typhoon FLA 9500	Amersham Imager 600
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*Equipement*	Advanced microscopy
2 photons FCS		SmFRET TCSPC	TIRF / 3D MFM	2 photons STED / FCS
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PALM
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Admin.Resp.	Florence Lepage
	(33) 04 67 41 79 04

Admin.Resp.	Didier Debain
	(33) 04 67 41 79 01

Manager.Assist.	Marion Boutin
	(33) 04 67 41 79 04

Centre de Biologie Structurale

Latest publications

GEF14 acts as a specific activator of the plant osmotic signaling pathway by controlling ROP6 nanodomain formation

A mechanism for ligand-dependent activation of AHR

A two-in-one expression construct for biophysical and structural studies of the human pregnane X receptor ligand-binding domain, a pharmaceutical and environmental target

An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice

Cryo-EM structure and regulation of human NAD kinase

Binding-Site Switch for Protein Kinase CK2 Inhibitors

Binding-site switch of Protein Kinase CK2 inhibitors

APH Inhibitors that Reverse Aminoglycoside Resistance in Enterococcus casseliflavus

Efficient Biochemical Method for Characterizing and Classifying Related Amyloidogenic Peptides

Microbes Saving Lives and Reducing Suffering

Site-Specific Incorporation of Fluorinated Prolines into Proteins and their Impact on Neighbouring Residues

Measuring Bacterial Flagellar Motor Dynamics via a Bead Assay

Determining the Relative Affinity of ORPs for Lipid Ligands Using Fluorescence and Thermal Shift Assays

What Structural Biology Tells Us About the Mode of Action and Detection of Toxicants

Structural Insights Into Thyroid Hormone Receptors

Structural basis of orientated asymmetry in a mGlu heterodimer

Structure of the aminoterminal domain of the birnaviral multifunctional VP3 protein and its unexplored critical role

Microbial biosensors for diagnostics, surveillance and epidemiology: Today's achievements and tomorrow's prospects

Towards the design of ligands of the internal pocket TEADs C-terminal domain

Weighted families of contact maps to characterize conformational ensembles of (highly-)flexible proteins

Biased activation of the vasopressin V2 receptor probed by molecular dynamics simulations, NMR and pharmacological studies

Molecular model of a bacterial flagellar motor in situreveals a parts-list of protein adaptations to increase torque

The flavonoid resokaempferol improves insulin secretion from healthy and dysfunctional pancreatic beta-cells

Split Reporters Facilitate Monitoring of Gene Expression and Peptide Production in Linear Cell-Free Transcription-Translation Systems

A molten globule ensemble primes Arf1-GDP for the nucleotide switch

The TOPOVIBL meiotic DSB formation protein: new insights from its biochemical and structural characterization

A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer-promoter communication

Quantifying surface tension and viscosity in biomolecular condensates by FRAP-ID

A fully sequenced collection of homozygous EMS mutants for forward and reverse genetic screens in Arabidopsis thaliana

@TOME 3.0: Interfacing Protein Structure Modeling and Ligand Docking

Photonic BioImaging

Photonic BioImaging

Fluorescence spectroscopy

Advanced microscopy

MARS - BioPhotonics

MARS - BioPhotonics

Fluorescence spectroscopy

Advanced microscopy

CBS Teams

Structure, Dynamics and Function of biomolecules by NMR

Structure and function of highly flexible proteins

Multi-scale structural biology

ABCIS

Nuclear Receptors as Integrators of Endogenous and Environmental Signals

Mechanisms of DNA Segregation and Remodelling

Physics and mechanics of biological systems

Integrative Biophysics of Membranes

Multiscale Biomolecular Modeling

Synthetic Biology

Chromosome structure and homologous recombination in meiosis

Functional and synthetic genomics

Structure and function of the aryl hydrocarbon receptor

Postdoctoral position – Team Integrative Biophysics of Membranes

Postdoctoral position – Team Integrative Biophysics of Membranes

Connexion