plateau

Photonic BioImaging

Platform

Photonic BioImaging

Technical manager

Caroline Clerté

Scientific manager

Christine Doucet

Permanent staff

Emmanuel Margeat, Jean-Bernard Fiche , Antoine Le Gall

The facility comprises 2 types of equipement:

Fluorescence spectroscopy plate readers, dedicated to fluorescence and luminescence characterization of biomacromolecular interactions or folding, enzymatic activity, protein expression… either from gels, solutions dispatched in multi-well plates, cells on petri dishes or multi-well plates. Some readers can do kinetics measurements, with or without injection.
Advanced, custom-built microscopes. Most of this equipment is operated by dedicated research engineers and projects are run on the basis of scientific collaborations.

Equipement

Fluorescence spectroscopy

MARS - BioPhotonics

*Platform*	MARS - BioPhotonics
*Technical manager*	Caroline Clerté
*Scientific manager*	Christine Doucet
*R&D managers*	Jean-Bernard Fiche , Antoine Le Gall
*Permanent staff*	Emmanuel Margeat

The facility offers a large range of equipment and expertise in fluorescence spectroscopy and/or microscopy. The facility is organized around three poles: Fluorescence spectroscopy plate readers. Advanced, custom-built microscopes. This equipment is operated by dedicated research engineers and projects are run on the basis of scientific collaborations. Research & Development division, that studies and proposes new implementations (technical or analytical) to address specific problems Our facility belongs to the FranceBioImaging Infrastructure

Services

Protein characterization	Measuring molecule diffusion	Measuring biomolecular interactions	Measuring protein structural dynamics
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Super-resolved 2D and 3D fluorescence imaging	MARS BioPhotonics R&D division
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Equipement

Fluorescence spectroscopy

Berthold Mithras LB940		CLARIOstar	Cytation 3	Synergy H1
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TECAN Safire2		Typhoon FLA 9500	Amersham Imager 600
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*Equipement*	Advanced microscopy
2 photons FCS		SmFRET TCSPC	TIRF / 3D MFM	2 photons STED / FCS
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PALM
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Admin.Resp.	Florence Lepage
	(33) 04 67 41 79 04

Admin.Resp.	Didier Debain
	(33) 04 67 41 79 01

Manager.Assist.	Marion Boutin
	(33) 04 67 41 79 04

Centre de Biologie Structurale

Latest publications

Redefining enhancer action: Insights from structural, genomic, and single-molecule perspectives

Triple Labeling Resolves a GPCR Intermediate State by Using Three-Color Single Molecule FRET

Editorial overview: Emerging perspectives in genome architecture and gene regulation

Molecular Assemblies of Amphiphilic Oligothiophenes at the Air-Water Interface

Structural and Functional Characterization of the 28kDa Structured Core of BmSA1, the Major Surface Antigen of Babesia Microti

Single-stranded DNA drives sigma subunit loading onto mycobacterial RNA polymerase to unlock initiation-competent conformations

Exploring RNA destabilization mechanisms in biomolecular condensates through atomistic simulations

Incoherent feedback from coupled amino acids and ribosome pools generates damped oscillations in growing E. coli

Structure of the turnip yellows virus particles

KDSAXS: A Tool for Analyzing Binding Equilibria With SAXS Data Using Explicit Models

Structure-guided insights into the biology of fungal effectors

Understanding the Relationship between Pressure and Temperature Unfolding of Proteins

GEF14 acts as a specific activator of the plant osmotic signaling pathway by controlling ROP6 nanodomain formation

A mechanism for ligand-dependent activation of AHR

AFflecto: A web server to generate conformational ensembles of flexible proteins from AlphaFold models

Binding-Site Switch for Protein Kinase CK2 Inhibitors

A two-in-one expression construct for biophysical and structural studies of the human pregnane X receptor ligand-binding domain, a pharmaceutical and environmental target

An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice

APH Inhibitors that Reverse Aminoglycoside Resistance in Enterococcus casseliflavus

Cryo-EM structure and regulation of human NAD kinase

Binding-site switch of Protein Kinase CK2 inhibitors

Efficient Biochemical Method for Characterizing and Classifying Related Amyloidogenic Peptides

Microbes Saving Lives and Reducing Suffering

Site-Specific Incorporation of Fluorinated Prolines into Proteins and their Impact on Neighbouring Residues

Measuring Bacterial Flagellar Motor Dynamics via a Bead Assay

Determining the Relative Affinity of ORPs for Lipid Ligands Using Fluorescence and Thermal Shift Assays

What Structural Biology Tells Us About the Mode of Action and Detection of Toxicants

Self-Assembled Peptide-Gold Nanoclusters with SiRNA Targeting Telomeric Response to Enhance Radiosensitivity in Lung Cancer Cells

Structural Insights Into Thyroid Hormone Receptors

Structural basis of orientated asymmetry in a mGlu heterodimer

Photonic BioImaging

Photonic BioImaging

Fluorescence spectroscopy

Advanced microscopy

MARS - BioPhotonics

MARS - BioPhotonics

Fluorescence spectroscopy

Advanced microscopy

CBS Teams

Structure, Dynamics and Function of biomolecules by NMR

Structure and function of highly flexible proteins

Multi-scale structural biology

ABCIS

Nuclear Receptors as Integrators of Endogenous and Environmental Signals

Mechanisms of DNA Segregation and Remodelling

Physics and mechanics of biological systems

Integrative Biophysics of Membranes

Multiscale Biomolecular Modeling

Synthetic Biology

Chromosome structure and homologous recombination in meiosis

Functional and synthetic genomics

Structure and function of the aryl hydrocarbon receptor

Postdoctoral position – Team Integrative Biophysics of Membranes

Postdoctoral position – Team Integrative Biophysics of Membranes

Connexion

K_DSAXS: A Tool for Analyzing Binding Equilibria With SAXS Data Using Explicit Models