Liste des publications

2023

• Concerted conformational changes control metabotropic glutamate receptor activity

  Lecat-Guillet N, Quast RB, Liu H, Bourrier E, Moller TC, Rovira X, Soldevila S, Lamarque L, Trinquet E, Liu J, Pin JP, Rondard P, Margeat E, Sci Adv, 2023

• Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae

  Hertzog M, Perry TN, Dupaigne P, Serres S, Morales V, Soulet AL, Bell JC, Margeat E, Kowalczykowski SC, Le Cam E, Fronzes R, Polard P, Nucleic Acids Res, 2023

• Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

  Agam G, Gebhardt C, Popara M, Machtel R, Folz J, Ambrose B, Chamachi N, Chung SY, Craggs TD, de Boer M, Grohmann D, Ha T, Hartmann A, Hendrix J, Hirschfeld V, Hubner CG, Hugel T, Kammerer D, Kang HS, Kapanidis AN, Krainer G, Kramm K, Lemke EA, Lerner E, Margeat E, Martens K, Michaelis J, Mitra J, Moya Munoz GG, Quast RB, Robb NC, Sattler M, Schlierf M, Schneider J, Schroeder T, Sefer A, Tan PS, Thurn J, Tinnefeld P, van Noort J, Weiss S, Wendler N, Zijlstra N, Barth A, Seidel CAM, Lamb DC, Cordes T, Nat Methods, 2023

2022

• Viro-fluidics: Real-time analysis of virus production kinetics at the single-cell level

  Eid J, Socol M, Naillon A, Feuillard J, Ciandrini L, Margeat E, Charlot B, Mougel M, Biophys Rep (N Y), 2022

• A modular spring-loaded actuator for mechanical activation of membrane proteins

  Mills A, Aissaoui N, Maurel D, Elezgaray J, Morvan F, Vasseur JJ, Margeat E, Quast RB, Lai-Kee-Him J, Saint N, Benistant C, Nord A, Pedaci F, Bellot G, Nat Commun, 2022

• Cryo-EM structure of transcription termination factor Rho from Mycobacterium tuberculosis reveals bicyclomycin resistance mechanism

  Saridakis E, Vishwakarma R, Lai-Kee-Him J, Martin K, Simon I, Cohen-Gonsaud M, Coste F, Bron P, Margeat E, Boudvillain M, Commun Biol, 2022

2021

• Allosteric modulators enhance agonist efficacy by increasing the residence time of a GPCR in the active state

  Cao AM, Quast RB, Fatemi F, Rondard P, Pin JP, Margeat E, Nat Commun, 2021

• Single-molecule FRET on its way to structural biology in live cells

  Quast RB, Margeat E, Nat Methods, 2021

• FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

  Lerner E, Barth A, Hendrix J, Ambrose B, Birkedal V, Blanchard SC, Borner R, Sung Chung H, Cordes T, Craggs TD, Deniz AA, Diao J, Fei J, Gonzalez RL, Gopich IV, Ha T, Hanke CA, Haran G, Hatzakis NS, Hohng S, Hong SC, Hugel T, Ingargiola A, Margeat E, Joo C, Kapanidis AN, Kim HD, Laurence T, Lee NK, Lee TH, Lemke EA, Michaelis J, Michalet X, Myong S, Nettels D, Peulen TO, Ploetz E, Razvag Y, Robb NC, Schuler B, Soleimaninejad H, Tang C, Vafabakhsh R, Lamb DC, Seidel CA, Weiss S, Elife, 2021

2020

• Author Correction: Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies

  Fernandes TFD, Saavedra-Villanueva O, Margeat E, Milhiet PE, Costa L, Sci Rep, 2020

• Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies

  Fernandes TFD, Saavedra O, Margeat E, Milhiet PE, Costa L, Sci Rep, 2020

2019

• Studying GPCR conformational dynamics by single molecule fluorescence

  Quast RB, Margeat E, Mol Cell Endocrinol, 2019

• Accurate Determination of Human CPR Conformational Equilibrium by smFRET using Dual Orthogonal Non-Canonical Amino Acid Labeling

  Quast RB, Fatemi F, Kranendonk M, Margeat E, Truan G, Chembiochem, 2019

• A fluorogenic assay to monitor Rho-dependent termination of transcription

  Nadiras C, Delaleau M, Schwartz A, Margeat E, Boudvillain M, Biochemistry, 2019

2018

• Chloride ions stabilize the glutamate-induced active state of the metabotropic glutamate receptor 3

  Tora AS, Rovira X, Cao AM, Cabaye A, Olofsson L, Malhaire F, Scholler P, Baik H, Van Eeckhaut A, Smolders I, Rondard P, Margeat E, Acher F, Pin JP, Goudet C, Neuropharmacology, 2018

• Oligomerization of a G protein-coupled receptor in neurons controlled by its structural dynamics

  Mller TC, Hottin J, Clerte C, Zwier JM, Durroux T, Rondard P, Prezeau L, Royer CA, Pin JP, Margeat E, Kniazeff J, Sci Rep, 2018

• Single-molecule analysis reveals the mechanism of transcription activation in

  Vishwakarma RK, Cao AM, Morichaud Z, Perumal AS, Margeat E, Brodolin K, Sci Adv, 2018

2017

• Competitive folding of RNA structures at a termination / antitermination site

  Ait-Bara S, Clerte C, Declerck N, Margeat E, RNA, 2017

2016

• Plasmonic Nanoantennas Enable Forbidden Forster Dipole-Dipole Energy Transfer and Enhance the FRET Efficiency

  de Torres J, Mivelle M, Moparthi SB, Rigneault H, van Hulst NF, Garcia-Parajo MF, Margeat E, Wenger J, Nano Lett, 2016

• Imaging HIV-1 RNA dimerization in cells by multicolor super-resolution and fluctuation microscopies

  Ferrer M, Clerte C, Chamontin C, Basyuk E, Laine S, Hottin J, Bertrand E, Margeat E, Mougel M, Nucleic Acids Res, 2016

• Optimisation of vectorisation property: a comparative study for a secondary amphipathic peptide

  Konate K, Lindberg M, Vaissiere A, Jourdan C, Aldrian G, Margeat E, Deshayes S, Boisguerin P, Int J Pharm, 2016

• Automatic detection of diffusion modes within biological membranes using back-propagation neural network

  Dosset P, Rassam P, Fernandez L, Espenel C, Rubinstein E, Margeat E, Milhiet PE, BMC Bioinformatics, 2016

2015

• Single-Molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein

  Ait-Bara S, Clerte C, Margeat E, Methods Mol Biol, 2015

• Direct observation of the translocation mechanism of transcription termination factor Rho

  Gocheva V, Le Gall A, Boudvillain M, Margeat E, Nollmann M, Nucleic Acids Res, 2015

2014

• Fine tuning of sub-millisecond conformational dynamics controls metabotropic glutamate receptors agonist efficacy

  Olofsson L, Felekyan S, Doumazane E, Scholler P, Fabre L, Zwier JM, Rondard P, Seidel CA, Pin JP, Margeat E, Nat Commun, 2014

• The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved Nucleotide Analog Interference Probing (trNAIP)

  Soares E, Schwartz A, Nollmann M, Margeat E, Boudvillain M, Nucleic Acids Res, 2014

• Chromatin Insulator Factors Involved in Long-Range DNA Interactions and Their Role in the Folding of the Drosophila Genome

  Vogelmann J, Le Gall A, Dejardin S, Allemand F, Gamot A, Labesse G, Cuvier O, Negre N, Cohen-Gonsaud M, Margeat E, Nollmann M, PLoS Genet, 2014

2013

• SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation

  Cattoni DI, Chara O, Godefroy C, Margeat E, Trigueros S, Milhiet PE, Nollmann M, EMBO Rep, 2013

• Competitive folding of anti-terminator/terminator hairpins monitored by single molecule FRET

  Clerte C, Declerck N, Margeat E, Nucleic Acids Res, 2013

• Negative regulation of estrogen signaling by ERbeta and RIP140 in ovarian cancer cells

  Docquier A, Garcia A, Savatier J, Boulahtouf A, Bonnet S, Bellet V, Busson M, Margeat E, Jalaguier S, Royer C, Balaguer P, Cavailles V, Mol Endocrinol, 2013

• Recruitment, assembly, and molecular architecture of the SpoIIIE DNA pump revealed by superresolution microscopy

  Fiche JB, Cattoni DI, Diekmann N, Langerak JM, Clerte C, Royer CA, Margeat E, Doan T, Nollmann M, PLoS Biol, 2013

• Pulsed interleaved excitation fluorescence spectroscopy with a supercontinuum source

  Olofsson L, Margeat E, Opt Express, 2013

2012

• Analysis of helicase-RNA interactions using nucleotide analog interference mapping

  Schwartz A, Rabhi M, Margeat E, Boudvillain M, Methods Enzymol, 2012

2011

• Mutagenesis-based evidence for an asymmetric configuration of the ring-shaped transcription termination factor Rho

  Rabhi M, Gocheva V, Jacquinot F, Lee A, Margeat E, Boudvillain M, J Mol Biol, 2011

• Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase

  Roumestand C, Leiba J, Galophe N, Margeat E, Padilla A, Bessin Y, Barthe P, Molle V, Cohen-Gonsaud M, Structure, 2011

2010

• Keeping up to speed with the transcription termination factor Rho motor

  Boudvillain M, Nollmann M, Margeat E, Transcription, 2010

• Transfert d'energie de Forster sur molecules uniques : applications en biologie

  Margeat E, L'Actualite Chimique, 2010

• HIV-1 assembly differentially alters dynamics and partitioning of tetraspanins and raft components

  Krementsov DN, Rassam P, Margeat E, Roy NH, Schneider-Schaulies J, Milhiet PE, Thali M, Traffic, 2010

2009

• Dynamic Partitioning of a Glycosyl-Phosphatidylinositol-Anchored Protein in Glycosphingolipid-Rich Microdomains Imaged by Single-Quantum Dot Tracking

  Pinaud F, Michalet X, Iyer G, Margeat E, Moore HP, Weiss S, Traffic, 2009

• A stepwise 2'-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase

  Schwartz A, Rabhi M, Jacquinot F, Margeat E, Rahmouni AR, Boudvillain M, Nat Struct Mol Biol, 2009

2008

• Quantitative analysis of the binding of ezrin to large unilamellar vesicles containing phosphatidylinositol 4,5 bisphosphate

  Blin G, Margeat E, Carvalho K, Royer CA, Roy C, Picart C, Biophys J, 2008

• Single-molecule analysis of CD9 dynamics and partitioning reveals multiple modes of interaction in the tetraspanin web

  Espenel C, Margeat E, Dosset P, Arduise C, Le Grimellec C, Royer CA, Boucheix C, Rubinstein E, Milhiet PE, J Cell Biol, 2008

• Investigating transcriptional regulation by fluorescence spectroscopy, from traditional methods to state-of-the-art single-molecule approaches

  Zorrilla S, Lillo MP, Chaix D, Margeat E, Royer CA, Declerck N, Ann N Y Acad Sci, 2008

2007

• The Characterization of Bio-molecular Interactions Using Fluorescence Fluctuation Techniques

  Margeat E, Boukari, H., Royer, C.A., Protein Reviews, 2007

• Transcription termination factor Rho can displace streptavidin from biotinylated RNA

  Schwartz A, Margeat E, Rahmouni AR, Boudvillain M, J Biol Chem, 2007

• Fructose-1,6-bisphosphate acts both as an inducer and as a structural cofactor of the central glycolytic genes repressor (CggR)

  Zorrilla S, Chaix D, Ortega A, Alfonso C, Doan T, Margeat E, Rivas G, Aymerich S, Declerck N, Royer CA, Biochemistry, 2007

• Inducer-modulated cooperative binding of the tetrameric CggR repressor to operator DNA

  Zorrilla S, Doan T, Alfonso C, Margeat E, Ortega A, Rivas G, Aymerich S, Royer CA, Declerck N, Biophys J, 2007

2006

• Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism

  Kapanidis AN, Margeat E, Ho SO, Kortkhonjia E, Weiss S, Ebright RH, Science, 2006

• Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes

  Margeat E, Kapanidis AN, Tinnefeld P, Wang Y, Mukhopadhyay J, Ebright RH, Weiss S, Biophys J, 2006

2005

• Retention of transcription initiation factor sigma70 in transcription elongation: single-molecule analysis

  Kapanidis AN, Margeat E, Laurence TA, Doose S, Ho SO, Mukhopadhyay J, Kortkhonjia E, Mekler V, Ebright RH, Weiss S, Mol Cell, 2005

• Alternating-laser excitation of single molecules

  Kapanidis AN, Laurence TA, Lee NK, Margeat E, Kong X, Weiss S, Acc Chem Res, 2005

• Carbocyanine dyes as efficient reversible single-molecule optical switch

  Heilemann M, Margeat E, Kasper R, Sauer M, Tinnefeld P, J Am Chem Soc, 2005

2004

• Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules

  Kapanidis AN, Lee NK, Laurence TA, Doose S, Margeat E, Weiss S, Proc Natl Acad Sci U S A, 2004

2003

• The H-NS dimerization domain defines a new fold contributing to DNA recognition

  Bloch V, Yang Y, Margeat E, Chavanieu A, Auge MT, Robert B, Arold S, Rimsky S, Kochoyan M, Nat Struct Biol, 2003

• Ligands differentially modulate the protein interactions of the human estrogen receptors alpha and beta

  Margeat E, Bourdoncle A, Margueron R, Poujol N, Cavailles V, Royer C, J Mol Biol, 2003

• RAR Antagonists Diminish the Level of DNA Binding by the RAR/RXR Heterodimer

  Poujol N, Margeat E, Baud S, Royer CA, Biochemistry, 2003

2002

• Equilibrium binding assays reveal the elevated stoichiometry and salt dependence of the interaction between full-length human sex-determining region on the Y chromosome (SRY) and DNA

  Baud S, Margeat E, Lumbroso S, Paris F, Sultan C, Royer C, Poujol N, J Biol Chem, 2002

• MabA (FabG1), a Mycobacterium tuberculosis protein involved in the long-chain fatty acid elongation system FAS-II

  Marrakchi H, Ducasse S, Labesse G, Montrozier H, Margeat E, Emorine L, Charpentier X, Daffe M, Quemard A, Microbiology, 2002

2001

• The human estrogen receptor alpha dimer binds a single SRC-1 coactivator molecule with an affinity dictated by agonist structure

  Margeat E, Poujol N, Boulahtouf A, Chen Y, Muller JD, Gratton E, Cavailles V, Royer CA, J Mol Biol, 2001

2000

• Quantitative characterization of the interaction between purified human estrogen receptor alpha and DNA using fluorescence anisotropy

  Boyer M, Poujol N, Margeat E, Royer CA, Nucleic Acids Res, 2000

1999

• Superoxide release by confluent endothelial cells, an electron spin resonance (ESR) study

  Barbacanne MA, Margeat E, Arnal JF, Nepveu F, Souchard JP, J. Chim. Phys., 1999

• Effect of ligand and DNA binding on the interaction between human transcription intermediary factor 1alpha and estrogen receptors

  Thenot S, Bonnet S, Boulahtouf A, Margeat E, Royer CA, Borgna JL, Cavailles V, Mol Endocrinol, 1999

1998

• Visualization of trp repressor and its complexes with DNA by atomic force microscopy

  Margeat E, Le Grimellec C, Royer CA, Biophys J, 1998

• Electron spin resonance detection of extracellular superoxide anion released by cultured endothelial cells

  Souchard JP, Barbacanne MA, Margeat E, Maret A, Nepveu F, Arnal JF, Free Radic Res, 1998

2007

• Alternating laser excitation of single molecules

  Kapanidis AN, Heilemann M, Margeat E, Kong X, Nir E, Weiss S, Single Molecule Techniques : A Laboratory Manual , 2007

2006

• The Characterization of Bio-molecular Interactions Using Fluorescence Fluctuation Techniques

  Margeat E, Boukari H, Royer CA, Protein Interactions, 2006