Measuring biomolecular interactions

1. Local concentrations and oligomerization states (N&B, FCS)
2. Binding between a fluorescent substrate and a partner (Fluorescence Anisotropy, FCS)
3. Binding between 2 fluorescent substrates (FCCS, smFRET, FLIM)
4. Changes in the local environment of fluorescent probes (Fluorescence Anisotropy, Fluorescence lifetime spectroscopy and imaging (FLIM))

All projects are run as collaborations. Please This email address is being protected from spambots. You need JavaScript enabled to view it. to help you choose the most appropriate approach. We also provide advice for the design of your experiment and support for fluorescent labeling and data analysis. After a meeting, we can conduct some experiments to assess the feasibility of the approach. Then a collaboration scheme is defined between the team and the facility. Our R&D division can also implement specific adaptations for your project if needed.

Intensity and brightness (inset) image by two-photon microscopy of a primary neuron transfected with SnapTag-mGlu2 labeled with Alexa488 (from Moller et al.)

Selected publications:

Møller T.C., Hottin J., Clerté C., Zwier J.M., Durroux T., Rondard P., Prezeau L., Royer C.A., Pin J.P., Margeat E., Kniazeff J. Oligomerization of a metabotropic glutamate receptor in neurons controlled by its structural dynamics, Scientifics Reports, 2018

Ferrer M, Clerte C, Chamontin C, Basyuk E, Laine S, Hottin J, Bertrand E, Margeat E, Mougel M, Imaging HIV-1 RNA dimerization in cells by multicolor super-resolution and fluctuation microscopies. Nucleic Acids Res, 2016

2 photons FCS

SmFRET TCSPC