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Station de pompage des porte-objets cryo Gatan à double entrée

Fluorescence

Equipement / Equipment

1- Fluorescence Spectroscopy :

Safire II micro plate reader Fluorimeter
Principal application : Absorption, Fluorescence scans, Anisotropy, Kinetics

Berthold micro plate reader (kinetics) Fluorimeter with injectors
Principal application : Absorption, Fluorescence scans, Anisotropy, Kinetics

Steady-State & Time Resolved Fluorimeter
Principal application : Steady state Excitation & Fluorescence scans, Anisotropy, Kinetics and Time Resolved Fluorescence & Anisotropy

High Pressure SpectroFluorimeter
High pressure fluorimeter ; Principal Application : effects of high pressure on protein folding.

2- Single Molecule Fluorescence Microscopy :

2 photon excitation FCS Microscope : Zeiss Axiovert 200 motorized microscope, Dual Channel ISS Alba Fluorescence correlation detector, Piezo electric microscope stage, Two photon excitation source (Spectra Physics Mai Tai HP) femtosecond tunable IR laser (from 700-1020 nm), Custom thermoregulated chamber, Galvo mirrors for scanning FCS.
Principal application : FCS, FCCS, Scanning Imaging, Circular scanning FCS & FCCS, N&B, RICS.

Confocal Microscope : Zeiss Axiovert 200 microscope, Multiline (476nm, 532nm, 633nm) alternating laser excitation (µsALEX) via Acousto-optic tunable filter, 3 channels APD-based multicoulour detection.
Principal Application : Single molecule FRET, 1 Photon FCS on molecules in solution.

TIRF Microscope : Zeiss Axiovert 200 microscope, Multiline (405nm, 457nm, 476nm, 488nm, 514nm, 532nm, 633nm) alternating laser excitation (msALEX) via Acousto-optic tunable filter, Prism based multicoulour Total Internal Reflection Microscopy (TIRF), Objective-type multicoulour Total Internal Reflection Microscopy (TIRF), Dual channel EMCCD-based detection with single molecule sensitivity.
Principal Application : Single molecule FRET on immobilized molecules, Epi Fluorescence Imaging, Single Particle Tracking.

3- Atomic Force Microscopy :

High-speed Atomic Force microscopy
High resolution imaging and topography of molecules and materials & manipulation of atoms and structures on a variety of surfaces. Observation in real time.

Multi mode AFM : Nanoscope® Va controller
L’AFM ; Principal application : High resolution imaging and topography of molecules and materials & manipulation of atoms and structures on a variety of surfaces.

AFM contact : Nanoscope® E controller

AFM Bioscope : Nanoscope® IIIa controller

AFM PicoII : PicoSPM II from Molecular Imaging

4- Others Biophysics instruments :

Circular Dichroism spectrophotometer Applied Photophysics Chirascan
le Circular dichroism ; principal application : investigate the secondary structure of proteins.

Microcalorimeter : 1- MICROCAL VP ITC
la microcalorimetry : Principal application : determine the thermodynamic parameters of molecule interactions in solution.

Microcalorimeter : 2- MICROCAL VP DSC

DLS Malvern Instrument Nano S
la DLS Principal Application : Determination of the distribution of diffusion coefficients of the particles, which are converted into a size distribution using established theories.

Admin.Resp.	Florence Lepage
	(33) 04 67 41 79 04

Admin.Resp.	Didier Debain
	(33) 04 67 41 79 01

Manager.Assist.	Marion Boutin
	(33) 04 67 41 79 04

Centre de Biologie Structurale

Latest publications

The structural landscape and diversity of Pyricularia oryzae MAX effectors revisited

Free-Standing DNA Origami Superlattice to Facilitate Cryo-EM Visualization of Membrane Vesicles

Multiplexed chromatin imaging reveals predominantly pairwise long-range coordination between Drosophila Polycomb genes

Phosphorylation of the F-BAR protein Hof1 drives septin ring splitting in budding yeast

1H, 13C and 15N backbone and side-chain resonance assignments of the human oncogenic protein NCYM

Development of new approach methods for the identification and characterization of endocrine metabolic disruptors-a PARC project

1H, 13C and 15N backbone and side-chain resonance assignments of the human oncogenic protein NCYM

Hi-M: A Multiplex Oligopaint FISH Method to Capture Chromatin Conformations In Situ and Accompanying Open-Source Acquisition Software

Emergence of slip-ideal-slip behavior in tip-links serve as force filters of sound in hearing

Increases in cyclin A/Cdk activity and in PP2A-B55 inhibition by FAM122A are key mitosis-inducing events

pyHiM: a new open-source, multi-platform software package for spatial genomics based on multiplexed DNA-FISH imaging

LactoSpanks: A Collection of IPTG Inducible Promoters for the Commensal Lactic Acid Bacteria Lactobacillus gasseri

PED in 2024: improving the community deposition of structural ensembles for intrinsically disordered proteins

An innovative in situAFM system for a soft X-ray spectromicroscopy synchrotron beamline

Critical micellar concentration determination of pure phospholipids and lipid-raft and their mixtures with cholesterol

Cancer selective cell death induction by a bivalent CK2 inhibitor targeting the ATP site and the allosteric alphaD pocket

Structural Insights into the Activation of Human Aryl Hydrocarbon Receptor by the Environmental Contaminant Benzo[a]pyrene and Structurally Related Compounds

Development of HC-258, a Covalent Acrylamide TEAD Inhibitor That Reduces Gene Expression and Cell Migration

The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population

A single-domain intrabody targeting the follicle-stimulating hormone receptor impacts FSH-induced G protein-dependent signalling

Structure-function relationships in protein homorepeats

3D chromatin interactions involving Drosophila insulators are infrequent but preferential and arise before TADs and transcription

A cyclin-dependent kinase-mediated phosphorylation switch of disordered protein condensation

wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments

Development of LM-41 and AF-2112, two flufenamic acid-derived TEAD inhibitors obtained through the replacement of the trifluoromethyl group by aryl rings

Adaptive evolution in virulence effectors of the rice blast fungus Pyricularia oryzae

Multi-scale dynamic imaging reveals that cooperative motility behaviors promote efficient predation in bacteria

Phosphorylation motif dictates GPCR C-terminal domain conformation and arrestin interaction

The novel cyclophilin inhibitor C105SR reduces hepatic ischaemia-reperfusion injury via mitoprotection

Site-Specific Introduction of Alanines for the Nuclear Magnetic Resonance Investigation of Low-Complexity Regions and Large Biomolecular Assemblies

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Station de pompage des porte-objets cryo Gatan à double entrée

Fluorescence

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