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Station de pompage des porte-objets cryo Gatan à double entrée

Fluorescence

Equipement / Equipment

1- Fluorescence Spectroscopy :

Safire II micro plate reader Fluorimeter
Principal application : Absorption, Fluorescence scans, Anisotropy, Kinetics

Berthold micro plate reader (kinetics) Fluorimeter with injectors
Principal application : Absorption, Fluorescence scans, Anisotropy, Kinetics

Steady-State & Time Resolved Fluorimeter
Principal application : Steady state Excitation & Fluorescence scans, Anisotropy, Kinetics and Time Resolved Fluorescence & Anisotropy

High Pressure SpectroFluorimeter
High pressure fluorimeter ; Principal Application : effects of high pressure on protein folding.

2- Single Molecule Fluorescence Microscopy :

2 photon excitation FCS Microscope : Zeiss Axiovert 200 motorized microscope, Dual Channel ISS Alba Fluorescence correlation detector, Piezo electric microscope stage, Two photon excitation source (Spectra Physics Mai Tai HP) femtosecond tunable IR laser (from 700-1020 nm), Custom thermoregulated chamber, Galvo mirrors for scanning FCS.
Principal application : FCS, FCCS, Scanning Imaging, Circular scanning FCS & FCCS, N&B, RICS.

Confocal Microscope : Zeiss Axiovert 200 microscope, Multiline (476nm, 532nm, 633nm) alternating laser excitation (µsALEX) via Acousto-optic tunable filter, 3 channels APD-based multicoulour detection.
Principal Application : Single molecule FRET, 1 Photon FCS on molecules in solution.

TIRF Microscope : Zeiss Axiovert 200 microscope, Multiline (405nm, 457nm, 476nm, 488nm, 514nm, 532nm, 633nm) alternating laser excitation (msALEX) via Acousto-optic tunable filter, Prism based multicoulour Total Internal Reflection Microscopy (TIRF), Objective-type multicoulour Total Internal Reflection Microscopy (TIRF), Dual channel EMCCD-based detection with single molecule sensitivity.
Principal Application : Single molecule FRET on immobilized molecules, Epi Fluorescence Imaging, Single Particle Tracking.

3- Atomic Force Microscopy :

High-speed Atomic Force microscopy
High resolution imaging and topography of molecules and materials & manipulation of atoms and structures on a variety of surfaces. Observation in real time.

Multi mode AFM : Nanoscope® Va controller
L’AFM ; Principal application : High resolution imaging and topography of molecules and materials & manipulation of atoms and structures on a variety of surfaces.

AFM contact : Nanoscope® E controller

AFM Bioscope : Nanoscope® IIIa controller

AFM PicoII : PicoSPM II from Molecular Imaging

4- Others Biophysics instruments :

Circular Dichroism spectrophotometer Applied Photophysics Chirascan
le Circular dichroism ; principal application : investigate the secondary structure of proteins.

Microcalorimeter : 1- MICROCAL VP ITC
la microcalorimetry : Principal application : determine the thermodynamic parameters of molecule interactions in solution.

Microcalorimeter : 2- MICROCAL VP DSC

DLS Malvern Instrument Nano S
la DLS Principal Application : Determination of the distribution of diffusion coefficients of the particles, which are converted into a size distribution using established theories.

Admin.Resp.	Florence Lepage
	(33) 04 67 41 79 04

Admin.Resp.	Didier Debain
	(33) 04 67 41 79 01

Manager.Assist.	Marion Boutin
	(33) 04 67 41 79 04

Centre de Biologie Structurale

Latest publications

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A two-in-one expression construct for biophysical and structural studies of the human pregnane X receptor ligand-binding domain, a pharmaceutical and environmental target

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Cryo-EM structure and regulation of human NAD kinase

Binding-Site Switch for Protein Kinase CK2 Inhibitors

Binding-site switch of Protein Kinase CK2 inhibitors

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Microbes Saving Lives and Reducing Suffering

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Determining the Relative Affinity of ORPs for Lipid Ligands Using Fluorescence and Thermal Shift Assays

What Structural Biology Tells Us About the Mode of Action and Detection of Toxicants

Structural Insights Into Thyroid Hormone Receptors

Structural basis of orientated asymmetry in a mGlu heterodimer

Structure of the aminoterminal domain of the birnaviral multifunctional VP3 protein and its unexplored critical role

Microbial biosensors for diagnostics, surveillance and epidemiology: Today's achievements and tomorrow's prospects

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Weighted families of contact maps to characterize conformational ensembles of (highly-)flexible proteins

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The flavonoid resokaempferol improves insulin secretion from healthy and dysfunctional pancreatic beta-cells

Split Reporters Facilitate Monitoring of Gene Expression and Peptide Production in Linear Cell-Free Transcription-Translation Systems

A molten globule ensemble primes Arf1-GDP for the nucleotide switch

The TOPOVIBL meiotic DSB formation protein: new insights from its biochemical and structural characterization

A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer-promoter communication

Quantifying surface tension and viscosity in biomolecular condensates by FRAP-ID

A fully sequenced collection of homozygous EMS mutants for forward and reverse genetic screens in Arabidopsis thaliana

@TOME 3.0: Interfacing Protein Structure Modeling and Ligand Docking

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Station de pompage des porte-objets cryo Gatan à double entrée

Fluorescence

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