Plateforme Intégrée de Biophysique et de Biologie Structurale


Cryo Electron Microscopy

Technical manager Joséphine Lai Kee Him
Scientific manager Pierre-Emmanuel Milhiet

During the last years, a revolution occurred in the EM field (stability of electron microscopes, advances in detector hardware and image-processing software) leading to determination of complex molecular structures at high resolution. Therefore, near-atomic-resolution cryoEM structures can now be obtained for even the most challenging targets in structural biology.

Examples of CryoEM samples observed. A : Purified CaMV (Cauliflower Mosaic Virus). B : Mixture of bacteriophages and TMV (Tobacco Mosaic Virus). C : Magnetic core silicium nanoparticles covered with a lipid bilayer. D : Treated polymers.

Structural analysis of frozen hydrated single particles


(Cryo) Electron tomography


Micro electron diffraction


Analysis or screening of negative stained sample

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  Negative stain
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