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Micro electron diffraction
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| Goal |
Structural analysis of tiny crystals by electron diffraction |
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Micro electron diffraction is a recently developed method in cryo electron microscopy that allow the collection of high resolution electron diffraction data from extremely small three-dimensional crystals that are in the range of 0,1 -0,4 mm thick using a transmission electron microscope. We implemented this approach on the JEOL 2200FS electron microscope and investigated its application to membrane protein crystals. |
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| Comment | Need to prepare crystals (see X-ray crystals preparation) then cryo EM sample freezing. | |
| Equipement |
Cryo Electron Microscope
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Vitrobot (ThermoFischer)
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Pumping Station (Gatan) for cryo holders
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