| Equipement |
Thermal Shift Assay |
|
| Measure |
Tm measurement |
|
|
96-well QPCR device using Sypro Orange fluorescent dye. Reaction volume 20 ul/well with a final protein concentration of 1 to 5 uM/well. Duration 80 min. |
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QPCR Agilent TechnologyTM Stratagene MX3005P |
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| Specifications | https://www.agilent.com/cs/library/usermanuals/public/70225.pdf | |
| Room | Z2-16@CBS29 | |
| Equipement |
Stopped-flow |
|
| Measure |
Absorbance, fluorescence, fluorescence anisotropy |
|
|
Double mixing stopped-flow allowing measurements of absorbance, intrinsic (for example tryptophan) or extrinsic (for example fluorescent probe) fluorescence or fluorescence anisotropy changes on the millisecond time scale. Temperature range : 4 to 40°C. |
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|
QPCR Agilent TechnologyTM Stratagene MX3005P |
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| Specifications | https://www.hi-techsci.com/instruments/kinetasyst-stopped-flow-systems/ | |
| Room | Z1-3@CBS29 | |
| Equipement |
Cryo Quench-flow |
|||
| Measure |
Enzyme kinetics |
|||
|
Cryo Quench-flow are used for cryoenzymic studies . Temperature range : -40 to 40°C. |
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Home-made Cryo Quench-flow |
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| Specifications | Please contact us | |||
| Room | Z1-3@CBS29 | |||
| Equipement |
Quench-flow |
|
| Measure |
Enzyme kinetics |
|
|
Quench-flow is a kinetic technique where a reaction is initiated by mixing two samples. The reaction ages in a delay line before being stopped after a user-defined ageing time, by mixing with a solution called the quencher. The aged solution is then collected at the exit of the quench-flow and is analyzed by external analytical methods, usually HPLC. Temperature range : 4 to 40°C. |
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|
QFM-400 BioLogic |
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| Specifications | https://www.biologic.net/products/qfm4000/ | |
| Room | Z1-3@CBS29 | |
