Centre de Biologie Structurale

 
logo teamc3

 

News

Seminars

  • 13 May
    LLPS Meeting 13 May 2024 11:00 AM to 12:00 PM

  • 16 May
    "Ask the ancestors: resurrecting and re-evolving the bacterial flagellar motor" Matthew Baker, University New South Wales, Sydney 16 May 2024 11:00 AM to 12:00 PM

    Matthew Baker,Scientia Associate Professor, UNSW Sydney (BABS), Faculty of Science.Visiting Professor, Institute of Molecular Biology and Biotechnology

  • 17 May
    Young CBS Researchers Meeting 17 May 2024 04:30 PM to 05:30 PM

  • 13 May - 17 May
  • 23 May - 27 May
  • 31 May - 06 Jun
  • 10 Jun - 14 Jun
  • 17 Jun - 24 Jun
  • 27 Jun - 01 Jul
  • 02 Jul - 05 Jul
‹ ›

Latest publications

View all publications

VP ITC Microcalorimetry

Equipement

VP ITC Microcalorimetry
Measure

Determination of affinity constants in protein/protein or protein/ligand or protein/DNA interactions. Determination of the stoichiometry of the biochemical interaction. Determination of thermodynamic constants such the enthalpy and the entropy.

Sample volume 2 ml at around 10-20 uM for the protein, 500 ul at 100-200 uM for the ligand. Duration 3 hours.
 

Isothermal titration calorimeter VP-ITC MicroCal (Malvern InstrumentsTM)

  
Specifications https://www.malvernpanalytical.com/fr/support/product-support/microcal-range/microcal-itc-range/microcal-vp-itc
Room Z2-16@CBS29

SEC-MALS

Equipement

SEC- MALS
Measure

Determination of molecular weight of proteins, protein/protein complexes, proteins/nucleic acids, nanodisks…Determination of protein oligomerization state and stoichiometry of complexes.

Sample volume 20 ul at 2 mg/ml, duration 1 hours. Different size exclusion columns available.
 

SEC –MALS : HPLC (AgilentTM Infinity II), Detector LS (miniDAWN TREOS – WayttTM), refractometry (Optilab T-rEX – WayttTM).

  
Specifications https://www.wyatt.com/products/instruments/minidawn-multi-angle-light-scattering-detector-sec-mals.html
https://www.wyatt.com/products/instruments/optilab-refractive-index-detector.html
Room Z1-5@CBS29

Nano DSF

Equipement

Nano DSF
Measure

Tm measurement

Simultaneous measurements of 6 different proteins or conditions. Sample volume 10 ul. Duration 3 min.
 

Nanotemper™ Tycho NT.6

  
Specifications https://nanotempertech.com/tycho/
Room Z2-16@CBS29

Circular Dichroism

Equipement

Circular Dichroism
Measure

Respective proportion estimation of the different secondary structure elements present in a protein.

Precision cells; light path 0,1 - 0,2 - 0,5 - 1 - 2 - 5 - 10 mm. Final protein concentration around 50-100 uM. Duration 10 min.
 

CD Chirascan (Applied Biophysics TM)

  
Specifications https://www.photophysics.com/systems/chirascan-systems/chirascan/system-information/
Room Z2-16@CBS29

More Articles ...

  1. Thermal Shift Assay
  2. Stopped-flow
  3. Quench-flow
  4. Cryo Quench-flow

CBS Teams

  • 1
 

Connexion