Centre de Biologie Structurale

News

Seminars

06 May

Conseil de Direction 06 May 2025 02:00 PM to 04:00 PM
12 May

Biophysics Seminar: Manu/Pem team 12 May 2025 11:00 AM to 12:00 PM
13 May

Introductory course on fluid mechanics & microfluidics -VI-Manouk Abkarian 13 May 2025 12:30 PM to 01:45 PM

06 May - 13 May
13 May - 15 May
16 May - 22 May
23 May - 02 Jun
03 Jun - 06 Jun
10 Jun - 16 Jun
19 Jun - 20 Jun

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Latest publications

GEF14 acts as a specific activator of the plant osmotic signaling pathway by controlling ROP6 nanodomain formation
Gorgues L, Smokvarska M, Mercier C, Igisch CP, Crabos A, Dongois A, Bayle V, Fiche JB, Nacry P, Nollmann M, Jaillais Y, Martiniere A, EMBO Rep, 2025
A mechanism for ligand-dependent activation of AHR
Guesdon A, Bourguet W, Trends Pharmacol Sci, 2025
A two-in-one expression construct for biophysical and structural studies of the human pregnane X receptor ligand-binding domain, a pharmaceutical and environmental target
Carivenc C, Laconde G, Blanc P, Amblard M, Bourguet W, Delfosse V, Acta Crystallogr F Struct Biol Commun, 2025
An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice
Wang X, Zhang G, Bian Z, Chow V, Grimaldi M, Carivenc C, Sirounian S, Li H, Sladekova L, Mott S, Luperi Y, Gong Y, Costello C, Li L, Jachimowicz M, Guo M, Hu S, Wilson D, Balaguer P, Bourguet W, Mani S, Bonati L, Peng H, March J, Wang H, Wang S, Krause HM, Liu J, Nat Commun, 2025
Cryo-EM structure and regulation of human NAD kinase
Praharaj PP, Li Y, Mary C, Soflaee MH, Ryu K, Kim D, Tran DH, Dey T, Tom HJ, Rion H, Gelin M, Lemoff A, Zacharias LG, Patricio JS, Mathews TP, Chen Z, Lionne C, Hoxhaj G, Labesse G, Sci Adv, 2025
Binding-Site Switch for Protein Kinase CK2 Inhibitors
Grenier D, Gelin M, Yang Y, Mularoni A, Guichou JF, Delcros JG, Krimm I, ChemMedChem, 2025
Binding-site switch of Protein Kinase CK2 inhibitors
Krimm I, Grenier D, Gelin M, Yang Cbs Cnrs Fr Y, Mularoni A, Guichou JF, Delcros JG, ChemMedChem, 2025
APH Inhibitors that Reverse Aminoglycoside Resistance in Enterococcus casseliflavus
Kaplan E, Chaloin L, Guichou JF, Berrou K, Rahimova R, Labesse G, Lionne C, ChemMedChem, 2025
Efficient Biochemical Method for Characterizing and Classifying Related Amyloidogenic Peptides
Pradeau-Phelut L, Alves S, Le Tareau L, Larralde C, Bernard E, Schirmer C, Lai-Kee-Him J, Lepvrier E, Bron P, Delamarche C, Garnier C, Anal Chem, 2025
Microbes Saving Lives and Reducing Suffering
Timmis K, Karahan ZC, Ramos JL, Koren O, Perez-Cobas AE, Steward K, de Lorenzo V, Caselli E, Douglas M, Schwab C, Rivero V, Giraldo R, Garmendia J, Turner RJ, Perlmutter J, Borrero de Acuna JM, Nikel PI, Bonnet J, Sessitsch A, Timmis JK, Pruzzo C, Prieto MA, Isazadeh S, Huang WE, Clarke G, Ercolini D, Haggblom M, Microb Biotechnol, 2025
Site-Specific Incorporation of Fluorinated Prolines into Proteins and their Impact on Neighbouring Residues
Elena-Real CA, Urbanek A, Sagar A, Mohanty P, Levy G, Morato A, Fournet A, Allemand F, Sibille N, Mittal J, Sinnaeve D, Bernado P, Chemistry, 2024
Measuring Bacterial Flagellar Motor Dynamics via a Bead Assay
Hoffmann WH, Biquet-Bisquert A, Pedaci F, Nord AL, Methods Mol Biol, 2024
Determining the Relative Affinity of ORPs for Lipid Ligands Using Fluorescence and Thermal Shift Assays
Delfosse V, Drin G, Methods Mol Biol, 2024
What Structural Biology Tells Us About the Mode of Action and Detection of Toxicants
le Maire A, Bourguet W, Annu Rev Pharmacol Toxicol, 2024
Structural Insights Into Thyroid Hormone Receptors
Tambones I, le Maire A, Endocrinology, 2024
Structural basis of orientated asymmetry in a mGlu heterodimer
Huang W, Jin N, Guo J, Shen C, Xu C, Xi K, Bonhomme L, Quast RB, Shen DD, Qin J, Liu YR, Song Y, Gao Y, Margeat E, Rondard P, Pin JP, Zhang Y, Liu J, Nat Commun, 2024
Structure of the aminoterminal domain of the birnaviral multifunctional VP3 protein and its unexplored critical role
Ferrero DS, Gimenez MC, Sagar A, Rodriguez JM, Caston JR, Terebiznik MR, Bernado P, Verdaguer N, PNAS Nexus, 2024
Microbial biosensors for diagnostics, surveillance and epidemiology: Today's achievements and tomorrow's prospects
Capin J, Chabert E, Zuniga A, Bonnet J, Microb Biotechnol, 2024
Towards the design of ligands of the internal pocket TEADs C-terminal domain
Toulotte F, Coevoet M, Liberelle M, Bailly F, Zagiel B, Gelin M, Allemand F, Fourquet P, Melnyk P, Guichou JF, Cotelle P, Eur J Med Chem, 2024
Weighted families of contact maps to characterize conformational ensembles of (highly-)flexible proteins
Gonzalez-Delgado J, Bernado P, Neuvial P, Cortes J, Bioinformatics, 2024
Biased activation of the vasopressin V2 receptor probed by molecular dynamics simulations, NMR and pharmacological studies
Fouillen A, Couvineau P, Gaibelet G, Riche S, Orcel H, Mendre C, Kanso A, Lanotte R, Nguyen J, Dimon J, Urbach S, Sounier R, Granier S, Bonnet D, Cong X, Mouillac B, Demene H, Comput Struct Biotechnol J, 2024
Molecular model of a bacterial flagellar motor in situreveals a parts-list of protein adaptations to increase torque
Drobnic T, Cohen EJ, Calcraft T, Alzheimer M, Froschauer K, Svensson S, Hoffmann WH, Singh N, Garg SG, Henderson L, Umrekar TR, Nans A, Ribardo D, Pedaci F, Nord AL, Hochberg GKA, Hendrixson DR, Sharma CM, Rosenthal PB, Beeby M, bioRxiv, 2024
The flavonoid resokaempferol improves insulin secretion from healthy and dysfunctional pancreatic beta-cells
Gautheron G, Peraldi-Roux S, Vaille J, Belhadj S, Patyra A, Bayle M, Youl E, Omhmmed S, Guyot M, Cros G, Guichou JF, Uzan B, Movassat J, Quignard JF, Neasta J, Oiry C, Br J Pharmacol, 2024
Split Reporters Facilitate Monitoring of Gene Expression and Peptide Production in Linear Cell-Free Transcription-Translation Systems
Levrier A, Capin J, Mayonove P, Karpathakis II, Voyvodic P, DeVisch A, Zuniga A, Cohen-Gonsaud M, Cabantous S, Noireaux V, Bonnet J, ACS Synth Biol, 2024
A molten globule ensemble primes Arf1-GDP for the nucleotide switch
Koduru T, Hantman N, Peters EV, Jaworek MW, Wang J, Zhang S, McCallum SA, Gillilan RE, Fossat MJ, Roumestand C, Sagar A, Winter R, Bernado P, Cherfils J, Royer CA, Proc Natl Acad Sci U S A, 2024
The TOPOVIBL meiotic DSB formation protein: new insights from its biochemical and structural characterization
Diagouraga B, Tambones I, Carivenc C, Bechara C, Nadal M, de Massy B, le Maire A, Robert T, Nucleic Acids Res, 2024
A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer-promoter communication
Denaud S, Bardou M, Papadopoulos GL, Grob S, Di Stefano M, Sabaris G, Nollmann M, Schuettengruber B, Cavalli G, Nat Struct Mol Biol, 2024
Quantifying surface tension and viscosity in biomolecular condensates by FRAP-ID
Santamaria A, Hutin S, Doucet CM, Zubieta C, Milhiet PE, Costa L, Biophys J, 2024
A fully sequenced collection of homozygous EMS mutants for forward and reverse genetic screens in Arabidopsis thaliana
Carrere S, Routaboul JM, Savourat P, Bellenot C, Lopez H, Sahoo A, Quiroz Monnens T, Ricou A, Camilleri C, Declerck N, Laufs P, Mercier R, Noel LD, Plant J, 2024
@TOME 3.0: Interfacing Protein Structure Modeling and Ligand Docking
Pons JL, Reys V, Grand F, Moreau V, Gracy J, Exner TE, Labesse G, J Mol Biol, 2024

View all publications

VP ITC Microcalorimetry

*Equipement*	VP ITC Microcalorimetry
*Measure*	Determination of affinity constants in protein/protein or protein/ligand or protein/DNA interactions. Determination of the stoichiometry of the biochemical interaction. Determination of thermodynamic constants such the enthalpy and the entropy.
Sample volume 2 ml at around 10-20 uM for the protein, 500 ul at 100-200 uM for the ligand. Duration 3 hours.
	Isothermal titration calorimeter VP-ITC MicroCal (Malvern InstrumentsTM)
*Specifications*	https://www.malvernpanalytical.com/fr/support/product-support/microcal-range/microcal-itc-range/microcal-vp-itc
*Room*	Z2-16@CBS29

SEC-MALS

*Equipement*	SEC- MALS
*Measure*	Determination of molecular weight of proteins, protein/protein complexes, proteins/nucleic acids, nanodisks…Determination of protein oligomerization state and stoichiometry of complexes.
Sample volume 20 ul at 2 mg/ml, duration 1 hours. Different size exclusion columns available.
	SEC –MALS : HPLC (AgilentTM Infinity II), Detector LS (miniDAWN TREOS – WayttTM), refractometry (Optilab T-rEX – WayttTM).
*Specifications*	https://www.wyatt.com/products/instruments/minidawn-multi-angle-light-scattering-detector-sec-mals.html https://www.wyatt.com/products/instruments/optilab-refractive-index-detector.html
*Room*	Z1-5@CBS29

Dynamic Light Scattering (DLS)

*Equipement*	Dynamic Light Scattering (DLS)
*Measure*	Molecule hydrodynamic radius measurement.
Measurement in microcuvettes. Reaction volume 40 ul with a final protein concentration around 100 uM. Duration 15 min.
	DLS Zetasizer Nano-S (Malvern Instrument TM)
*Specifications*	https://www.malvernpanalytical.com/fr/products/product-range/zetasizer-range/zetasizer-nano-range/zetasizer-nano-s
*Room*	Z2-16@CBS29

Nano DSF

*Equipement*	Nano DSF
*Measure*	Tm measurement
Simultaneous measurements of 6 different proteins or conditions. Sample volume 10 ul. Duration 3 min.
	Nanotemper™ Tycho NT.6
*Specifications*	https://nanotempertech.com/tycho/
*Room*	Z2-16@CBS29

Circular Dichroism

*Equipement*	Circular Dichroism
*Measure*	Respective proportion estimation of the different secondary structure elements present in a protein.
Precision cells; light path 0,1 - 0,2 - 0,5 - 1 - 2 - 5 - 10 mm. Final protein concentration around 50-100 uM. Duration 10 min.
	CD Chirascan (Applied Biophysics TM)
*Specifications*	https://www.photophysics.com/systems/chirascan-systems/chirascan/system-information/
*Room*	Z2-16@CBS29

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CBS Teams

Structure, Dynamics and Function of biomolecules by NMR

Christian Roumestand and Nathalie Declerck
Structure and function of highly flexible proteins

Pau Bernadó and Nathalie Sibille
Multi-scale structural biology

Patrick Bron
ABCIS

Gilles Labesse
Nuclear Receptors as Integrators of Endogenous and Environmental Signals

William Bourguet and Albane Le Maire
Multiscale Biomolecular Modeling

Alessandro Barducci
Mechanisms of DNA Segregation and Remodelling

Marcelo Nollmann
Physics and mechanics of biological systems

Manouk Abkarian and Francesco Pedaci
Integrative Biophysics of Membranes

Pierre-Emmanuel Milhiet and Emmanuel Margeat
Synthetic Biology

Jérôme Bonnet and Martin Cohen-Gonsaud
Multiscale Biomolecular Modeling

Alessandro Barducci
Chromosome structure and homologous recombination in meiosis

Thomas Robert
Functional and synthetic genomics

Guillaume Cambray
Structure and function of the aryl hydrocarbon receptor

Jakub Gruszczyk

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