Tools

SelenoCYS

A protocol for the incorporation of selenomethionine and selenocysteine into recombinant proteins overexpressed in Escherichia coli based on the use of a suitable cysteine auxotrophic strain.

Strub MP., Hoh F., Sanchez JF., Strub JM., Böck A., Aumelas A. & Dumas C. (2003) "Selenomethionine and Selenocysteine Double Labeling Strategy for Crystallographic Phasing", Structure , 11, 1359-1367.

SUPERFLIP

Adapting the Charge Flipping algorithm to biological macromolecule diffraction data :
* ab initio determination of protein crystal structures at atomic resolution
* heavy atom or anomalous scatterers substructure determination

Dumas C. & van der Lee A. (2008) "Macromolecular structure solution by charge flipping", Acta Cryst D64, 864-873.

Palatinus L. & Chapuis G. (2007) "SUPERFLIP, a computer program for the solution of crystal structures by charge flipping in arbitrary dimensions", J. Appl. Cryst.40,786-790.

CINDY

A graphical NMR assignment software.
André PADILLA (CBS)

CRAACK

Consensus Rules for Amino-Acid Characterisation using a Kernel method (SVM).
The spin system typing consensus is based on the following softwares : Rescue, RescueN, Rescue2, Platon, and one other using SVM algorithm.
Benod C., Delsuc MA., Pons JL (2006) J. Chem. Inf. Model. 46 (3) 1517-1522

Additional Sponsors : ACI-IMPBio

EDMON v3

Endocrine Disruptor MONitoring - A computational tool to study interactions between nuclear hormone receptors and endocrine-disrupting chemicals
Delfosse et al, PNAS, 2012 - Doi : 10.1073/pnas.1203574109

Additional Sponsors : ANR BISCOT

Admin.Resp.	Florence Lepage
	(33) 04 67 41 79 04

Admin.Resp.	Didier Debain
	(33) 04 67 41 79 01

Manager.Assist.	Marion Boutin
	(33) 04 67 41 79 04

Centre de Biologie Structurale

Latest publications

Redefining enhancer action: Insights from structural, genomic, and single-molecule perspectives

Triple Labeling Resolves a GPCR Intermediate State by Using Three-Color Single Molecule FRET

Editorial overview: Emerging perspectives in genome architecture and gene regulation

Molecular Assemblies of Amphiphilic Oligothiophenes at the Air-Water Interface

Structural and Functional Characterization of the 28kDa Structured Core of BmSA1, the Major Surface Antigen of Babesia Microti

Single-stranded DNA drives sigma subunit loading onto mycobacterial RNA polymerase to unlock initiation-competent conformations

Exploring RNA destabilization mechanisms in biomolecular condensates through atomistic simulations

Incoherent feedback from coupled amino acids and ribosome pools generates damped oscillations in growing E. coli

Structure of the turnip yellows virus particles

KDSAXS: A Tool for Analyzing Binding Equilibria With SAXS Data Using Explicit Models

Structure-guided insights into the biology of fungal effectors

Understanding the Relationship between Pressure and Temperature Unfolding of Proteins

GEF14 acts as a specific activator of the plant osmotic signaling pathway by controlling ROP6 nanodomain formation

A mechanism for ligand-dependent activation of AHR

AFflecto: A web server to generate conformational ensembles of flexible proteins from AlphaFold models

Binding-Site Switch for Protein Kinase CK2 Inhibitors

A two-in-one expression construct for biophysical and structural studies of the human pregnane X receptor ligand-binding domain, a pharmaceutical and environmental target

An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice

APH Inhibitors that Reverse Aminoglycoside Resistance in Enterococcus casseliflavus

Cryo-EM structure and regulation of human NAD kinase

Binding-site switch of Protein Kinase CK2 inhibitors

Efficient Biochemical Method for Characterizing and Classifying Related Amyloidogenic Peptides

Microbes Saving Lives and Reducing Suffering

Site-Specific Incorporation of Fluorinated Prolines into Proteins and their Impact on Neighbouring Residues

Measuring Bacterial Flagellar Motor Dynamics via a Bead Assay

Determining the Relative Affinity of ORPs for Lipid Ligands Using Fluorescence and Thermal Shift Assays

What Structural Biology Tells Us About the Mode of Action and Detection of Toxicants

Self-Assembled Peptide-Gold Nanoclusters with SiRNA Targeting Telomeric Response to Enhance Radiosensitivity in Lung Cancer Cells

Structural Insights Into Thyroid Hormone Receptors

Structural basis of orientated asymmetry in a mGlu heterodimer

SelenoCYS

SUPERFLIP

CINDY

CRAACK

EDMON v3

More Articles ...

CBS Teams

Structure, Dynamics and Function of biomolecules by NMR

Structure and function of highly flexible proteins

Multi-scale structural biology

ABCIS

Nuclear Receptors as Integrators of Endogenous and Environmental Signals

Mechanisms of DNA Segregation and Remodelling

Physics and mechanics of biological systems

Integrative Biophysics of Membranes

Multiscale Biomolecular Modeling

Synthetic Biology

Chromosome structure and homologous recombination in meiosis

Functional and synthetic genomics

Structure and function of the aryl hydrocarbon receptor

Postdoctoral position – Team Integrative Biophysics of Membranes

Postdoctoral position – Team Integrative Biophysics of Membranes

Connexion

K_DSAXS: A Tool for Analyzing Binding Equilibria With SAXS Data Using Explicit Models