 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
Matthew Baker,Scientia Associate Professor, UNSW Sydney (BABS), Faculty of Science.Visiting Professor, Institute of Molecular Biology and Biotechnology
Mouhand A, Nakatani K, Kono F, Hippo Y, Matsuo T, Barthe P, Peters J, Suenaga Y, Tamada T, Roumestand C, Biomol NMR Assign, 2024
Fiche JB, Schaeffer M, Houbron C, Elkhoury Youhanna C, Messina O, Barho F, Nollmann M, Methods Mol Biol, 2024
Arora N, Hazra JP, Roy S, Bhati GK, Gupta S, Yogendran KP, Chaudhuri A, Sagar A, Rakshit S, Nat Commun, 2024
Lacroix B, Vigneron S, Labbe JC, Pintard L, Lionne C, Labesse G, Castro A, Lorca T, EMBO J, 2024
Devos X, Fiche JB, Bardou M, Messina O, Houbron C, Gurgo J, Schaeffer M, Goetz M, Walter T, Mueller F, Nollmann M, Genome Biol, 2024
| Equipement |
Thermal Shift Assay |
|
| Measure |
Tm measurement |
|
|
96-well QPCR device using Sypro Orange fluorescent dye. Reaction volume 20 ul/well with a final protein concentration of 1 to 5 uM/well. Duration 80 min. |
||
QPCR Agilent TechnologyTM Stratagene MX3005P |
||
| Specifications | https://www.agilent.com/cs/library/usermanuals/public/70225.pdf | |
| Room | Z2-16@CBS29 | |
| Equipement |
Stopped-flow |
|
| Measure |
Absorbance, fluorescence, fluorescence anisotropy |
|
|
Double mixing stopped-flow allowing measurements of absorbance, intrinsic (for example tryptophan) or extrinsic (for example fluorescent probe) fluorescence or fluorescence anisotropy changes on the millisecond time scale. Temperature range : 4 to 40°C. |
||
|
QPCR Agilent TechnologyTM Stratagene MX3005P |
||
| Specifications | https://www.hi-techsci.com/instruments/kinetasyst-stopped-flow-systems/ | |
| Room | Z1-3@CBS29 | |
| Equipement |
Cryo Quench-flow |
|||
| Measure |
Enzyme kinetics |
|||
|
Cryo Quench-flow are used for cryoenzymic studies . Temperature range : -40 to 40°C. |
||||
Home-made Cryo Quench-flow |
||||
| Specifications | Please contact us | |||
| Room | Z1-3@CBS29 | |||
| Equipement |
Quench-flow |
|
| Measure |
Enzyme kinetics |
|
|
Quench-flow is a kinetic technique where a reaction is initiated by mixing two samples. The reaction ages in a delay line before being stopped after a user-defined ageing time, by mixing with a solution called the quencher. The aged solution is then collected at the exit of the quench-flow and is analyzed by external analytical methods, usually HPLC. Temperature range : 4 to 40°C. |
||
|
QFM-400 BioLogic |
||
| Specifications | https://www.biologic.net/products/qfm4000/ | |
| Room | Z1-3@CBS29 | |
Christian Roumestand and Nathalie Declerck
Pau Bernadó and Nathalie Sibille
Patrick Bron
Gilles Labesse
William Bourguet and Albane Le Maire
Alessandro Barducci
Marcelo Nollmann
Manouk Abkarian and Francesco Pedaci
Pierre-Emmanuel Milhiet and Emmanuel Margeat
Jérôme Bonnet and Martin Cohen-Gonsaud
Alessandro Barducci
Thomas Robert
Guillaume Cambray
Jakub Gruszczyk
