Micro electron diffraction
Structural analysis of tiny crystals by electron diffraction
Micro electron diffraction is a recently developed method in cryo electron microscopy that allow the collection of high resolution electron diffraction data from extremely small three-dimensional crystals that are in the range of 0,1 -0,4 mm thick using a transmission electron microscope. We implemented this approach on the JEOL 2200FS electron microscope and investigated its application to membrane protein crystals.
|Need to prepare crystals (see X-ray crystals preparation) then cryo EM sample freezing.