Plateforme Intégrée de Biophysique et de Biologie Structurale


Micro electron diffraction


Structural analysis of tiny crystals by electron diffraction

Micro electron diffraction is a recently developed method in cryo electron microscopy that allow the collection of high resolution electron diffraction data from extremely small three-dimensional crystals that are in the range of 0,1 -0,4 mm thick using a transmission electron microscope. We implemented this approach on the JEOL 2200FS electron microscope and investigated its application to membrane protein crystals.


Electron diffraction pattern of Lysozyme micro-crystal and its computed structure

Comment Need to prepare crystals (see X-ray crystals preparation) then cryo EM sample freezing.

Cryo Electron Microscope


Vitrobot (ThermoFischer)


Pumping Station (Gatan) for cryo holders


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