Structure et fonction de protéines hautement flexibles

Chefs d'équipe : Pau Bernadó & Nathalie Sibille 

Members of the Team:

Researchers

• Pau Bernadó, INSERM
• Nathalie Sibille, CNRS

Engineers and technicians

• Frédéric Allemand, CNRS
• Aurélie Fournet, INSERM

Visiting scientist

• Aurélien Thureau (Eng SOLEIL)

Non-permanent

• Ons Dakhlaoui, Post-Doc ANR
• Xamuel Lund, PhD ILL
• Ilinka Clerc, PhD LAAS Toulouse
• Sylvain Kumanski, AI ANR
• Maud Magne--Repossi, M1 IBIS Montpellier

Aims of the group: The underlying concept in the structure-function paradigm is that a rigid 3D structure is necessary to perform a biological task. Recent findings have demonstrated that flexibility is crucial for the functioning of biomolecules. Biophysical characterization of highly flexible biomolecules such as IDPs is a challenge for traditional structural biology methods. We propose to address these challenges by integrative approaches where structural and dynamic information derived from different structural biology methods will be integrated into computational tools to achieve more complete models that can provide direct functional insights. The two principal techniques we use are NMR and SAXS. High magnetic fields (>700 MHz) are mandatory to address the challenges posed by IDPs. This line of research will be led by PB and NS that will address two biological systems.

Areas of Expertise: Nuclear Magnetic Resonance, Small-Angle X-ray Scattering, Intrinsically Disordered Proteins, Residual Dipolar Couplings, Structural Modelling.

Funding:

ANR « CORNFLEX » (2022-2026) ANR « NanoGold » (2022-2026) LabMUSE EpiGenMed Proof of Concept «  Ki67 project » (2022-2023) ANR « DropINOut » (2021-2024) MUSE « Ondine » (2021-2023) Institut Laue-Langevin (ILL-Grenoble) PhD grant (2020-2023) MSCA-RISE: "REFRACT (2019-2023) ANR GPCteR (2018-2022) ERC-CoG (2015-2020) Labex EpiGenMed FRM Postdoc grant (2016-2017) SUDOE-NEUROMED (2014-2015) ATIP-AVENIR (2012-2015) SPIN-HD: ANR-CHEX-2011. (2012-2015)

Former members:

• Carlos Elena-Real (Post-Doc ANR 2018-2023)
• Natan-David Levy (M1 BIOTIN 2023)
• Assia Mouhand (Post-Doc ANR GPCteR 2018-2023)
• Amin Sagar (Post-Doc Labex EpiGenMed 2018-2022)
• Flora Dechosal (M1 Muse Ki67 2022)
• Santigo Rodriguez (PhD student Universidad Nacional de la Plata – MSCA-Rise REFRACT - visit 4 months in 2022)
• Myriam Guillien (PhD Labex/ANR GPCteR 2018-2021)
• Elodie Roux (BTS ANR GPCteR 2021-2022)
• Annika Urbanek (Pdoc ERC 2015-2020)
• Anna Morató (Eng ERC 2015-2020)
• Alejandro Estaña (PhD ERC 2015-2020)
• Matija Popovic (Pdoc ERC 2017-2019)
• Lucile Sénicourt (Pdoc Labex NR 2017-2019)
• Grayson Gerlich (Bsc RPI 2019)
• Vincent Meynier (M1 ANR GPCteR 2018)
• Belen Chaves (PhD student at CSIC Madrid with José Perez-Cañadillas – visit 4 months in 2018)
• Karen Zhang (Bsc Princeton 2017)
• Elise Delaforge (Pdoc ERC 2016-2017)
• Fatima Herranz (PhD ERC 2013-2017)
• Amandine Gontier (M1&M2 GPCteR 2015/2016)
• Tiago Cordeiro (Pdoc Atip 2012-2016)
• Susana Barrera-Vilarmau (PhD student at CSIC Barcelona with Ramon Crehuet – visit 6 months in 2015)
• Aleix Martí (Erasmus GPCteR 2014)