| Service |
Correlative AFM with Super-Resolution, TIRF or Confocal/FLIM
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| Goal |
Correlate biomolecules activity observed by Fluorescence (Epi and TIRF), Super Resolution Microscopy (PALM/STORM) or Confocal and Fluorescence Spectroscopy (FLIM, FRET, FCS) with the associated sample topology and mechanics observed by AFM. |
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Our two fluorescence-AFM correlative setups are mounted on top of Zeiss inverted microscopes. Fast AFM imaging (1 frame in few seconds) can be performed simultaneously with the fluorescence microscopy operation. |
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Amphiphatic Lipid Packing Sensors (ALPS, left), and DOPE (Rhodamin marked, center) TIRF image correlated with the AFM image of the model membrane + biomolecules on the right. Scan size = 10 µm. |
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| Equipement |
Correlative AFM with Super-Resolution Fluorescence, TIRF and EPI
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Correlative AFM with Fluorescence Spectroscopy
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