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Publications
2026
Correction: The structural landscape and diversity of Pyricularia oryzae MAX effectors revisited
Lahfa M, Barthe P, deGuillen K, Cesari S, Raji M, Kroj T, Le Naour-Vernet M, Hoh F, Gladieux P, Roumestand C, Gracy J, Declerck N, Padilla A, PLoS Pathog, 2026
Two folds, many faces: The Magnaporthe oryzae MAX effector AVR-Pia targets novel rice HMA domain-containing proteins
Maidment JHR, Saile SC, Bocquet A, Thivolle C, Bourcet L, Planel LF, Gelin M, Kroj T, Padilla A, deGuillen K, Cesari S, PLoS Pathog, 2026
Pilot metaproteomic profiling reveals bacterial diversity and potential medical and veterinary relevance of tick microbiomes in northern Algeria
Kernif T, Lozano C, Khardine FA, Medrouh B, Hachid A, Fernandez B, Eddaikra N, Delbecq S, Armengaud J, Sereno D, Holzmuller P, Sci Rep, 2026
Differential Properties of NS1 Glycoproteins in West Nile and Usutu Viruses
Revel J, Leroy J, Delbecq S, Constant O, Marty F, Naili C, Barthes A, Nagy A, Schmidt-Chanasit J, Cadar D, Abd Rahaman NY, Lajoix AD, Desmetz C, Simonin Y, Emerg Microbes Infect, 2026
Corrigendum to Adaptation of Folding and Function of a Nuclease from the Cold Deep Sea [J. Mol. Biol. 438(4) (2026) 169602]
Koduru T, Barthe P, Hantman N, Guillen K, McCallum SA, Morgan JE, Yee EF, Leonardi P, Foland J, Roumestand C, Royer CA, J Mol Biol, 2026
Revealing protein dynamics with high-pressure NMR
Roche J, Roumestand C, Royer CA, Q Rev Biophys, 2026
2025
Adaptation of Folding and Function of a Nuclease from the Cold Deep Sea
Koduru T, Barthe P, Hantman N, deGuillen K, McCallum SA, Morgan JE, Yee EF, Leonardi P, Foland J, Roumestand C, Royer CA, J Mol Biol, 2025
Zymoseptoria tritici Effectors Structurally Related to Killer Proteins UmV-KP4 and UmV-KP6 Inhibit Fungal Growth, and Define Extended Protein Families in Fungi
deGuillen K, Mammri L, Gracy J, Padilla A, Barthe P, Hoh F, Lahfa M, Rouffet J, Petit-Houdenot Y, Kroj T, Lebrun MH, Mol Plant Pathol, 2025
Structural and Functional Characterization of the 28kDa Structured Core of BmSA1, the Major Surface Antigen of Babesia Microti
Mouhand A, Pissarra J, Barthe P, Roumestand C, Delbecq S, Proteins, 2025
Structure-guided insights into the biology of fungal effectors
Le Naour-Vernet M, Lahfa M, Maidment JHR, Padilla A, Roumestand C, deGuillen K, Kroj T, Cesari S, New Phytol, 2025
Understanding the Relationship between Pressure and Temperature Unfolding of Proteins
Roumestand C, Dudas E, Puglisi R, Calio A, Barthe P, Temussi PA, Pastore A, JACS Au, 2025
2024
A molten globule ensemble primes Arf1-GDP for the nucleotide switch
Koduru T, Hantman N, Peters EV, Jaworek MW, Wang J, Zhang S, McCallum SA, Gillilan RE, Fossat MJ, Roumestand C, Sagar A, Winter R, Bernado P, Cherfils J, Royer CA, Proc Natl Acad Sci U S A, 2024
A fully sequenced collection of homozygous EMS mutants for forward and reverse genetic screens in Arabidopsis thaliana
Carrere S, Routaboul JM, Savourat P, Bellenot C, Lopez H, Sahoo A, Quiroz Monnens T, Ricou A, Camilleri C, Declerck N, Laufs P, Mercier R, Noel LD, Plant J, 2024
The structural landscape and diversity of Pyricularia oryzae MAX effectors revisited
Lahfa M, Barthe P, deGuillen K, Cesari S, Raji M, Kroj T, Le Naour-Vernet M, Hoh F, Gladieux P, Roumestand C, Gracy J, Declerck N, Padilla A, PLoS Pathog, 2024
1H, 13C and 15N backbone and side-chain resonance assignments of the human oncogenic protein NCYM
Mouhand A, Nakatani K, Kono F, Hippo Y, Matsuo T, Barthe P, Peters J, Suenaga Y, Tamada T, Roumestand C, Biomol NMR Assign, 2024
2023
Does a Similar 3D Structure Mean a Similar Folding Pathway? The Presence of a C-Terminal alpha-Helical Extension in the 3D Structure of MAX60 Drastically Changes the Folding Pathway Described for Other MAX-Effectors from Magnaporthe oryzae
Lahfa M, Mouhand A, deGuillen K, Barthe P, Kroj T, Padilla A, Roumestand C, Molecules, 2023
1H, 13C and 15N backbone and side-chain resonance assignments of BmSA1, the surface antigen of Babesia microti
Mouhand A, Pissarra J, Delbecq S, Roumestand C, Barthe P, Biomol NMR Assign, 2023
Bioaccumulated provitamin A in black soldier fly larvae is bioavailable and capable of improving vitamin A status of gerbils
Morand-Laffargue L, Delbecq S, Creton B, Sabatier D, Papin M, Dhuique-Mayer C, Borel P, Food Res Int, 2023
A Mycobacterium tuberculosis Effector Targets Mitochondrion, Controls Energy Metabolism, and Limits Cytochrome cExit
Martin M, deVisch A, Boudehen YM, Barthe P, Gutierrez C, Turapov O, Aydogan T, Heriaud L, Gracy J, Neyrolles O, Mukamolova GV, Letourneur F, Cohen-Gonsaud M, Microbiol Spectr, 2023
Freeze-drying of few microliters of antibody formulations to implement 384-wells homogeneous instant assays
Coussot G, Le Postollec G, Delbecq S, Dobrijevic M, Analytica Chimica Acta, 2023
2022
PYK2 senses calcium through a disordered dimerization and calmodulin-binding element
Momin AA, Mendes T, Barthe P, Faure C, Hong S, Yu P, Kadare G, Jaremko M, Girault JA, Jaremko , Arold ST, Commun Biol, 2022
Unravelling the Adaptation Mechanisms to High Pressure in Proteins
Calio A, Dubois C, Fontanay S, Koza MM, Hoh F, Roumestand C, Oger P, Peters J, Int J Mol Sci, 2022
1H, 13C, 15N backbone and side-chain NMR assignments for three MAX effectors from Magnaporthe oryzae
Lahfa M, Padilla A, deGuillen K, Pissarra J, Raji M, Cesari S, Kroj T, Gladieux P, Roumestand C, Barthe P, Biomol NMR Assign, 2022
Combining High-Pressure NMR and Geometrical Sampling to Obtain a Full Topological Description of Protein Folding Landscapes: Application to the Folding of Two MAX Effectors from Magnaporthe oryzae
Dubois C, Lahfa M, Pissarra J, deGuillen K, Barthe P, Kroj T, Roumestand C, Padilla A, Int J Mol Sci, 2022
New recognition specificity in a plant immune receptor by molecular engineering of its integrated domain
Cesari S, Xi Y, Declerck N, Chalvon V, Mammri L, Pugniere M, Henriquet C, deGuillen K, Chochois V, Padilla A, Kroj T, Nat Commun, 2022
Major Surface Antigens in Zoonotic Babesia
Delbecq S, Pathogens, 2022
2021
Paracrine Behaviors Arbitrate Parasite-Like Interactions Between Tumor Subclones
Noble RJ, Walther V, Roumestand C, Hochberg ME, Hibner U, Lassus P, Front Ecol Evol, 2021
Comparative Assessment of NMR Probes for the Experimental Description of Protein Folding Pathways with High-Pressure NMR
Van Deuren V, Yang YS, deGuillen K, Dubois C, Royer CA, Roumestand C, Barthe P, Biology (Basel), 2021
The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop
Labbe JC, Vigneron S, Mechali F, Robert P, Roque S, Genoud C, Goguet-Rubio P, Barthe P, Labesse G, Cohen-Gonsaud M, Castro A, Lorca T, Nat Commun, 2021
Protein-Ligand Binding Volume Determined from a Single 2D NMR Spectrum with Increasing Pressure
Skvarnavicius G, Toleikis Z, Michailoviene V, Roumestand C, Matulis D, Petrauskas V, J Phys Chem B, 2021
Pressure and Chemical Unfolding of an alpha-Helical Bundle Protein: The GH2 Domain of the Protein Adaptor GIPC1
Dubois C, Planelles-Herrero VJ, Tillatte-Tripodi C, Delbecq S, Mammri L, Sirkia EM, Ropars V, Roumestand C, Barthe P, Int J Mol Sci, 2021
Resolving the activation mechanism of the D99N antiterminator LicT protein
Yang Y, Gracy J, Declerck N, Demene H, J Struct Biol, 2021
Modular Imaging Scaffold for Single-Particle Electron Microscopy
Aissaoui N, Lai-Kee-Him J, Mills A, Declerck N, Morichaud Z, Brodolin K, Baconnais S, Le Cam E, Charbonnier JB, Sounier R, Granier S, Ropars V, Bron P, Bellot G, ACS Nano, 2021
2020
Combining High-Pressure Perturbation with NMR Spectroscopy for a Structural and Dynamical Characterization of Protein Folding Pathways
Dubois C, Herrada I, Barthe P, Roumestand C, Molecules, 2020
An oligomeric switch controls the Mrr-induced SOS response in E. coli
Bourges AC, Torres Montaguth OE, Tadesse W, Labesse G, Aertsen A, Royer CA, Declerck N, DNA Repair (Amst), 2020
Quantitative High-Resolution Imaging of Live Microbial Cells at High Hydrostatic Pressure
Bourges AC, Lazarev A, Declerck N, Rogers KL, Royer CA, Biophys J, 2020
Flanking Regions Determine the Structure of the Poly-Glutamine in Huntingtin through Mechanisms Common among Glutamine-Rich Human Proteins
Urbanek A, Popovic M, Morato A, Estana A, Elena-Real CA, Mier P, Fournet A, Allemand F, Delbecq S, Andrade-Navarro MA, Cortes J, Sibille N, Bernado P, Structure, 2020
NMR chemical shift assignment of a constitutively active fragment of the antitermination protein LicT
Yang Y, Declerck N, Demene H, Biomol NMR Assign, 2020
Structural Insights into of the Allosteric Activation of the LicT Antiterminator by PTS-Mediated Phosphorylation
Yang Y, Padilla A, deGuillen K, Mammri L, Gracy J, Declerck N, Demene H, Structure, 2020
2019
Site-Specific Isotopic Labeling (SSIL) - Access to High-Resolution Structural and Dynamic Information in Low Complexity Proteins
Urbanek A, Elena-Real CA, Popovic M, Morato A, Fournet A, Allemand F, Delbecq S, Sibille N, Bernado P, Chembiochem, 2019
Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds
deGuillen K, Lorrain C, Tsan P, Barthe P, Petre B, Saveleva N, Rouhier N, Duplessis S, Padilla A, Hecker A, Sci Rep, 2019
Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112
Alqaseer K, Turapov O, Barthe P, Jagatia H, De Visch A, Roumestand C, Wegrzyn M, Bartek IL, Voskuil MI, O'Hare HM, Ajuh P, Bottrill AR, Witney AA, Cohen-Gonsaud M, Waddell SJ, Mukamolova GV, Mol Microbiol, 2019
Babesia divergens glycosylphosphatidylinositols modulate blood coagulation and induce Th2-biased cytokine profiles in antigen presenting cells
Debierre-Grockiego F, Smith TK, Delbecq S, Ducournau C, Lantier L, Schmidt J, Bres V, Dimier-Poisson I, Schwarz RT, Cornillot E, Biochimie, 2019
Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression
Cordeiro TN, Sibille N, Germain P, Barthe P, Boulahtouf A, Allemand F, Bailly R, Vivat V, Ebel C, Barducci A, Bourguet W, le Maire A, Bernado P, Structure, 2019
Folding of the Ig-Like Domain of the Dengue Virus Envelope Protein Analyzed by High-Hydrostatic-Pressure NMR at a Residue-Level Resolution
Saotome T, Doret M, Kulkarni M, Yang YS, Barthe P, Kuroda Y, Roumestand C, Biomolecules, 2019
Structural basis for chemically-induced homodimerization of a single domain antibody
Lesne J, Chang HJ, De Visch A, Paloni M, Barthe P, Guichou JF, Mayonove P, Barducci A, Labesse G, Bonnet J, Cohen-Gonsaud M, Sci Rep, 2019
Vaccination against babesiosis using recombinant GPI-anchored proteins
Nathaly Wieser S, Schnittger L, Florin-Christensen M, Delbecq S, Schetters T, Int J Parasitol, 2019
Quantitative high-resolution imaging of live microbial cells at high hydrostatic pressure
Bourges AC, Lazarev A, Declerck N, Rogers KL, Royer CA, bioRxiv, 2019
Exploring Protein Conformational Landscapes Using High-Pressure NMR
Roche J, Royer CA, Roumestand C, Meth Enzymol, 2019
2018
Towards the total synthesis of trichormamide A, a cyclic undecapeptide
Gaillard S, Das S, Djibo M, Raviglione D, Roumestand C, Legrand B, Inguimbert N, Tetrahedron Letters, 2018
Specific recognition of two MAX effectors by integrated HMA domains in plant immune receptors involves distinct binding surfaces
Guo L, Cesari S, deGuillen K, Chalvon V, Mammri L, Ma M, Meusnier I, Bonnot F, Padilla A, Peng YL, Liu J, Kroj T, Proc Natl Acad Sci USA, 2018
Two Faces of CwlM, an Essential PknB Substrate, in Mycobacterium tuberculosis
Turapov O, Forti F, Kadhim B, Ghisotti D, Sassine J, Straatman-Iwanowska A, Bottrill AR, Moynihan PJ, Wallis R, Barthe P, Cohen-Gonsaud M, Ajuh P, Vollmer W, Mukamolova GV, Cell Rep, 2018
A Tumor-Imaging Method Targeting Cancer-Associated Fibroblasts
Loktev A, Lindner T, Mier W, Debus J, Altmann A, Jager D, Giesel F, Kratochwil C, Barthe P, Roumestand C, Haberkorn U, J Nucl Med, 2018
Monitoring Unfolding of Titin I27 Single and Bi Domain with High-Pressure NMR Spectroscopy
Herrada I, Barthe P, Vanheusden M, DeGuillen K, Mammri L, Delbecq S, Rico F, Roumestand C, Biophys J, 2018
A General Strategy to Access Structural Information at Atomic Resolution in Polyglutamine Homorepeats
Urbanek A, Morato A, Allemand F, Delaforge E, Fournet A, Popovic M, Delbecq S, Sibille N, Bernado P, Angew Chem Int Ed Engl, 2018
A General Strategy to Access Atomic Resolution Structural Information in Poly-Glutamine Homo-Repeats
Bernado P, Urbanek A, Morato A, Allemand F, Delaforge E, Fournet A, Popovic M, Delbecq S, Sibille N, Angew Chem Int Ed Engl, 2018
2017
Monitoring protein folding through high pressure NMR spectroscopy
Roche J, Royer CA, Roumestand C, Prog Nucl Magn Reson Spectrosc, 2017
High pressure activation of the Mrr restriction endonuclease in Escherichia coli involves tetramer dissociation
Bourges AC, Torres Montaguth OE, Ghosh A, Tadesse WM, Declerck N, Aertsen A, Royer CA, Nucleic Acids Res, 2017
Competitive folding of RNA structures at a termination / antitermination site
Ait-Bara S, Clerte C, Declerck N, Margeat E, RNA, 2017
Recognition of the Magnaporthe oryzae effector AVR-Pia by the decoy domain of the rice NLR immune receptor RGA5
Ortiz D, deGuillen K, Cesari S, Chalvon V, Gracy J, Padilla A, Kroj T, Plant Cell, 2017
2016
High-Resolution Mapping of a Repeat Protein Folding Free Energy Landscape
Fossat MJ, Dao TP, Jenkins K, Dellarole M, Yang Y, McCallum SA, Garcia AE, Barrick D, Roumestand C, Royer CA, Biophys J, 2016
Mycobacterium tuberculosis LppM Displays an Original Structure and Domain Composition Linked to a Dual Localization
Barthe P, Veyron-Churlet R, de Visch A, Gilleron M, Saliou JM, Tomavo S, Nigou J, Brodin P, Cohen-Gonsaud M, Structure, 2016
Volume of Hsp90 Protein-Ligand Binding Determined by Fluorescent Pressure Shift Assay, Densitometry, and NMR
Toleikis Z, Sirotkin VA, Skvarnavicius G, Smirnoviene J, Roumestand C, Matulis D, Petrauskas V, J Phys Chem B, 2016
A part toolbox to tune genetic expression in Bacillus subtilis
Guiziou S, Sauveplane V, Chang HJ, Clerte C, Declerck N, Jules M, Bonnet J, Nucleic Acids Res, 2016
2015
Structure Analysis Uncovers a Highly Diverse but Structurally Conserved Effector Family in Phytopathogenic Fungi
deGuillen K, Ortiz-Vallejo D, Gracy J, Fournier E, Kroj T, Padilla A, PLoS Pathog, 2015
Evolutionarily Conserved Pattern of Interactions in a Protein Revealed by Local Thermal Expansion Properties
Dellarole M, Caro JA, Roche J, Fossat M, Barthe P, Garcia-Moreno E B, Royer CA, Roumestand C, J Am Chem Soc, 2015
Exploring the Protein Folding Pathway with High-Pressure NMR: Steady-State and Kinetics Studies
Roche J, Dellarole M, Royer CA, Roumestand C, Subcell Biochem, 2015
The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth
Turapov O, Loraine J, Jenkins CH, Barthe P, McFeely D, Forti F, Ghisotti D, Hesek D, Lee M, Bottrill AR, Vollmer W, Mobashery S, Cohen-Gonsaud M, Mukamolova GV, Open Biol, 2015
2014
(1)H, (15)N and (13)C Backbone resonance assignments of murine met-neuroglobin, free and in complex with cyanide
Yang Y, Allemand F, Guca E, Vallone B, Delbecq S, Roumestand C, Biomol NMR Assign, 2014
Prioritizing targets for structural biology through the lens of proteomics: The archaeal protein TGAM_1934 from Thermococcus gammatolerans
Yang YS, Fernandez B, Lagorce A, Aloin V, deGuillen KM, Boyer JB, Dedieu A, Confalonieri F, Armengaud J, Roumestand C, Proteomics, 2014
2013
A disulfide-constrained miniprotein with striking tumor-binding specificity developed by ribosome display
Zoller F, Markert A, Barthe P, Hebling U, Altmann A, Lindner T, Mier W, Haberkorn U, Angew Chem Int Ed Engl, 2013
Measuring residual dipolar couplings at high hydrostatic pressure: robustness of alignment media to high pressure
Sibille N, Dellarole M, Royer C, Roumestand C, J Biomol NMR, 2013
Kinetics of interaction between ADP-ribosylation factor-1 (Arf1) and the Sec7 domain of Arno guanine nucleotide exchange factor, modulation by allosteric factors, and the uncompetitive inhibitor brefeldin A
Rouhana J, Padilla A, Estaran S, Bakari S, Delbecq S, Boublik Y, Chopineau J, Pugniere M, Chavanieu A, J Biol Chem, 2013
Effect of internal cavities on folding rates and routes revealed by real-time pressure-jump NMR spectroscopy
Roche J, Dellarole M, Caro JA, Norberto DR, Garcia AE, Garcia-Moreno B, Roumestand C, Royer CA, J Am Chem Soc, 2013
Structural, energetic, and dynamic responses of the native state ensemble of staphylococcal nuclease to cavity-creating mutations
Roche J, Caro JA, Dellarole M, Guca E, Royer CA, Garcia-Moreno BE, Garcia AE, Roumestand C, Proteins, 2013
Low-cost equilibrium unfolding of heme proteins using 2 mul samples
Guca E, Roumestand C, Vallone B, Royer CA, Dellarole M, Anal Biochem, 2013
Equivalence between Euler angle conventions for the description of tensorial interactions in liquid NMR: application to different software programs
Dosset P, Barthe P, Cohen-Gonsaud M, Roumestand C, Demene H, J Biomol NMR, 2013
Volumetric properties underlying ligand binding in a monomeric hemoglobin: a high-pressure NMR study
Dellarole M, Roumestand C, Royer C, Lecomte JT, Biochim Biophys Acta, 2013
Interactions in gene expression networks studied by two-photon fluorescence fluctuation spectroscopy
Declerck N, Royer CA, Meth Enzymol, 2013
Competitive folding of anti-terminator/terminator hairpins monitored by single molecule FRET
Clerte C, Declerck N, Margeat E, Nucleic Acids Res, 2013
1H, 1aN and 13C Backbone resonance assignments of a conformational mutant of the adhesion protein a-Bd37 from Babesia divergens
Barthe P, Murciano B, Schetters T, Gorenflot A, Delbecq S, Roumestand C, Biomol NMR Assign, 2013
The RYMV-encoded viral suppressor of RNA silencing P1 is a zinc-binding protein with redox-dependent flexibility
Gillet FX, Cattoni DI, Petiot-Becard S, Delalande F, Poignavent V, Brizard JP, Bessin Y, Dorsselaer AV, Declerck N, Sanglier-Cianferani S, Brugidou C, Vignols F, J Mol Biol, 2013
2012
Absolute quantification of gene expression in individual bacterial cells using two-photon fluctuation microscopy
Ferguson ML, Le Coq D, Jules M, Aymerich S, Declerck N, Royer CA, Anal Biochem, 2012
Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states
Ferguson ML, Le Coq D, Jules M, Aymerich S, Radulescu O, Declerck N, Royer CA, Proc Natl Acad Sci U S A, 2012
Cavities determine the pressure unfolding of proteins
Roche J, Caro JA, Norberto DR, Barthe P, Roumestand C, Schlessman JL, Garcia AE, Garcia-Moreno BE, Royer CA, Proc Natl Acad Sci U S A, 2012
Remodeling of the folding free energy landscape of staphylococcal nuclease by cavity-creating mutations.
Roche J, Dellarole M, Caro JA, Guca E, Norberto DR, Yang Y, Garcia AE, Roumestand C, Garcia-Moreno B, Royer CA, Biochemistry, 2012
Structural and functional characterization of Bc28.1, major erythrocyte-binding protein from Babesia canis merozoite surface
Yang YS, Murciano B, Moubri K, Cibrelus P, Schetters T, Gorenflot A, Delbecq S, Roumestand C, J Biol Chem, 2012
Combination of phage display and molecular grafting generates highly specific tumor-targeting miniproteins.
Zoller F, Markert A, Barthe P, Zhao W, Weichert W, Askoxylakis V, Altmann A, Mier W, Haberkorn U, Angew. Chem. Int. Ed. Engl., 2012
2011
Structural plasticity of staphylococcal nuclease probed by perturbation with pressure and pH
Kitahara R, Hata K, Maeno A, Akasaka K, Chimenti MS, Garcia-Moreno EB, Schroer MA, Jeworrek C, Tolan M, Winter R, Roche J, Roumestand C, Montet deGuillen K, Royer CA, Proteins, 2011
Structure of the Mycobacterium tuberculosis OmpATb protein: a model of an oligomeric channel in the mycobacterial cell wall
Yang Y, Auguin D, Delbecq S, Dumas E, Molle G, Molle V, Roumestand C, Saint N, Proteins, 2011
Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase
Roumestand C, Leiba J, Galophe N, Margeat E, Padilla A, Bessin Y, Barthe P, Molle V, Cohen-Gonsaud M, Structure, 2011
Prevention of cross-talk in conserved regulatory systems: identification of specificity determinants in RNA-binding anti-termination proteins of the BglG family
Hubner S, Declerck N, Diethmaier C, Le Coq D, Aymerich S, Stulke J, Nucleic Acids Res., 2011
2010
Combination of noncovalent mass spectrometry and traveling wave ion mobility spectrometry reveals sugar-induced conformational changes of central glycolytic genes repressor/DNA complex
Atmanene C, Chaix D, Bessin Y, Declerck N, Van Dorsselaer A, Sanglier-Cianferani S, Anal Chem, 2010
The structure of PknB extracellular PASTA domain from mycobacterium tuberculosis suggests a ligand-dependent kinase activation
Barthe P, Mukamolova GV, Roumestand C, Cohen-Gonsaud M, Structure, 2010
Physical basis of the inducer-dependent cooperativity of the Central glycolytic genes Repressor/DNA complex
Chaix D, Ferguson ML, Atmanene C, Van Dorsselaer A, Sanglier-Cianferani S, Royer CA, Declerck N, Nucleic Acids Res, 2010
2009
Dynamic and structural characterization of a bacterial FHA protein reveals a new autoinhibition mechanism
Barthe P, Roumestand C, Canova MJ, Kremer L, Hurard C, Molle V, Cohen-Gonsaud M, Structure, 2009
The Mycobacterium tuberculosis Ser/Thr kinase substrate Rv2175c is a DNA-binding protein regulated by phosphorylation
Cohen-Gonsaud M, Barthe P, Canova MJ, Stagier-Simon C, Kremer L, Roumestand C, Molle V, J Biol Chem, 2009
Intrinsic disorder in Viral Proteins Genome-Linked: experimental and predictive analyses
Hebrard E, Bessin Y, Michon T, Longhi S, Uversky VN, Delalande F, Van Dorsselaer A, Romero P, Walter J, Declerck N, Fargette D, Virol J, 2009
Structural studies of the complex between Akt-in and the Akt2-PH domain suggest that the peptide acts as an allosteric inhibitor of the Akt kinase
Ropars V, Barthe P, Wang CS, Chen W, Tzou DL, Descours A, Martin L, Noguchi M, Roumestand C, Open Spect J, 2009
The TCL1A oncoprotein interacts directly with the NF-kappaB inhibitor IkappaB
Ropars V, Despouy G, Stern MH, Benichou S, Roumestand C, Arold ST, PLoS One, 2009
2008
Characterization of the control catabolite protein of gluconeogenic genes repressor by fluorescence cross-correlation spectroscopy and other biophysical approaches
Zorrilla S, Ortega A, Chaix D, Alfonso C, Rivas G, Aymerich S, Lillo MP, Declerck N, Royer CA, Biophys J, 2008
Investigating transcriptional regulation by fluorescence spectroscopy, from traditional methods to state-of-the-art single-molecule approaches
Zorrilla S, Lillo MP, Chaix D, Margeat E, Royer CA, Declerck N, Ann N Y Acad Sci, 2008
Analysis of the C12A-p8MTCP1 protein internal motions using Fast Density Mapping at multiple magnetic fields
Ropars V, Roche J, Barthe P, Roumestand C, C.R. Chimie, 2008
Structural mechanism of signal transduction between the RNA-binding domain and the phosphotransferase system regulation domain of the LicT antiterminator
Demene H, Ducat T, DeGuillen K, Birck C, Aymerich S, Kochoyan M, Declerck N, J Biol Chem, 2008
The solution structure of the adhesion protein Bd37 from Babesia divergens reveals structural homology with eukaryotic proteins involved in membrane trafficking
Delbecq S, Auguin D, Yang YS, Lohr F, Arold ST, Schetters T, Precigout E, Gorenflot A, Roumestand C, J Mol Biol, 2008
Molecular basis for group-specific activation of the virulence regulator PlcR by PapR heptapeptides
Bouillaut L, Perchat S, Arold ST, Zorrilla S, Slamti L, Henry C, Gohar M, Declerck N, Lereclus D, Nucleic Acids Res, 2008
A phospho-sugar binding domain homologous to NagB enzymes regulates the activity of the central glycolytic genes repressor
Doan T, Martin L, Zorrilla S, Chaix D, Aymerich S, Labesse G, Declerck N, Proteins, 2008
2007
Structural analysis of cassiicolin, a host-selective protein toxin from Corynespora cassiicola
Barthe P, Pujade-Renaud V, Breton F, Gargani D, Thai R, Roumestand C, de Lamotte F, J Mol Biol, 2007
Structure of PlcR: Insights into virulence regulation and evolution of quorum sensing in Gram-positive bacteria
Declerck N, Bouillaut L, Chaix D, Rugani N, Slamti L, Hoh F, Lereclus D, Arold ST, Proc Natl Acad Sci U S A, 2007
Proto-oncogene TCL1: more than just a coactivator for Akt
Noguchi M, Ropars V, Roumestand C, Suizu F, Faseb J, 2007
Unraveling protein dynamics through fast spectral density mapping
Ropars V, Bouguet-Bonnet S, Auguin D, Barthe P, Canet D, Roumestand C, J Biomol NMR, 2007
Fructose-1,6-bisphosphate acts both as an inducer and as a structural cofactor of the central glycolytic genes repressor (CggR)
Zorrilla S, Chaix D, Ortega A, Alfonso C, Doan T, Margeat E, Rivas G, Aymerich S, Declerck N, Royer CA, Biochemistry, 2007
Inducer-modulated cooperative binding of the tetrameric CggR repressor to operator DNA
Zorrilla S, Doan T, Alfonso C, Margeat E, Ortega A, Rivas G, Aymerich S, Royer CA, Declerck N, Biophys J, 2007
2006
Structural basis for the Akt (PKB) kinase inhibition by the peptide Akt-in: an NMR study.
Ropars V, Guichou J-F, Auguin D, Barthe P, Noguchi M, Roumestand C, C. R. Chimie, 2006
Rapid determination of protein solubility and stability conditions for NMR studies using incomplete factorial design
Ducat T, Declerck N, Gostan T, Kochoyan M, Demene H, J Biomol NMR, 2006
DYNAMOF: a program for the dynamics analysis of relaxation data obtained at multiple magnetic fields.
Barthe P, Ropars V, Roumestand C, C. R. Chimie, 2006
2005
The concept of effective correlation times for describing backbone motions in proteins. Part I. A residue-per-residue self-consistent analysis of multifield N-15 relaxation parameters
Bouguet-Bonnet S, Mutzenhardt P, Roumestand C, Canet D, Concept in Magnetic Resonance Part A, 2005
The concept of effective correlation times for describing backbone motions in proteins. Part II. Tentative interpretation of the residue-specific correlation time in terms of overall rotation-diffusion
Bouguet-Bonnet S, Mutzenhardt P, Roumestand C, Canet D, Concept in Magnetic Resonance Part A, 2005
The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes
Cohen-Gonsaud M, Barthe P, Bagneris C, Henderson B, Ward J, Roumestand C, Keep NH, Nat Struct Mol Biol, 2005
Activation of the LicT transcriptional antiterminator involves a domain swing/lock mechanism provoking massive structural changes
Graille M, Zhou CZ, Receveur-Brechot V, Collinet B, Declerck N, van Tilbeurgh H, J Biol Chem, 2005
Scorpion-toxin mimics of CD4 in complex with human immunodeficiency virus gp120 crystal structures, molecular mimicry, and neutralization breadth
Huang CC, Stricher F, Martin L, Decker JM, Majeed S, Barthe P, Hendrickson WA, Robinson J, Roumestand C, Sodroski J, Wyatt R, Shaw GM, Vita C, Kwong PD, Structure, 2005
A natural variant of Bacillus licheniformis alpha-amylase isolated from flour mill wastewaters sheds light on the origin of high thermostability
Shahhoseini M, Ziaee AA, Pourbabai AA, Ghaemi N, Declerck N, J Appl Microbiol, 2005
A high-throughput fluorescence polarization assay specific to the CD4 binding site of HIV-1 glycoproteins based on a fluorescein-labelled CD4 mimic
Stricher F, Martin L, Barthe P, Pogenberg V, Mechulam A, Menez A, Roumestand C, Veas F, Royer C, Vita C, Biochem J, 2005
1H, 15N and 13C Backbone resonance assignments of the 37 kDa surface antigen protein Bd37 from Babesia divergens
Yang YS, Delbecq S, Strub MP, Lohr F, Schetters T, Gorenflot A, Precigout E, Roumestand C, J Biomol NMR, 2005
2004
Design of an amphipatic a-helical hairpin peptide
Barthe P, Cohen-Gonsaud M, Aldrian-Herrada G, Chavanieu A, Labesse G, Roumestand C, Comptes Rendus Chimie, 2004
Inhibition of Akt kinase activity by a peptide spanning the betaA strand of the proto-oncogene TCL1
Hiromura M, Okada F, Obata T, Auguin D, Shibata T, Roumestand C, Noguchi M, J Biol Chem, 2004
(1)H, (15)N, and (13)C chemical shift assignments of the resuscitation promoting factor domain of Rv1009 from Mycobacterium tuberculosis
Cohen-Gonsaud M, Barthe P, Pommier F, Harris R, Driscoll PC, Keep NH, Roumestand C, J Biomol NMR, 2004
Determination de l'etat d'oligomerisation du domaine homologue a la pleckstrine de la proteine AKT2 par des techniques RMN
Auguin D, Gostan T, MA D, Roumestand C, C.R.A.S. Chimie, 2004
Solution structure and backbone dynamics of the pleckstrin homology domain of the human protein kinase B (PKB/Akt). Interaction with inositol phosphates
Auguin D, Barthe P, Auge-Senegas MT, Stern MH, Noguchi M, Roumestand C, J Biomol NMR, 2004
Structural basis for the co-activation of protein kinase B by T-cell leukemia-1 (TCL1) family proto-oncoproteins
Auguin D, Barthe P, Royer C, Stern MH, Noguchi M, Arold ST, Roumestand C, J Biol Chem, 2004
NMR determination of the Pleckstrin Homology domain oligomerisation state of the protein Akt2
Auguin D, Gostan T, Delsuc M-A, Roumestand C, C.R. Chimie, 2004
2003
1H, (15)N and (13)C chemical shift assignments of the Pleckstrin Homology domain of the Human Protein Kinase B (Pkb/Akt)
Auguin D, Barthe P, Auge-Senegas MT, Hoh F, Noguchi M, Roumestand C, J. Biomol. NMR, 2003
Hyperthermostabilization of Bacillus licheniformis alpha-amylase and modulation of its stability over a 50 degrees C temperature range
Declerck N, Machius M, Joyet P, Wiegand G, Huber R, Gaillardin C, Protein Eng, 2003
Kinetic stabilization of Bacillus licheniformis alpha-amylase through introduction of hydrophobic residues at the surface
Machius M, Declerck N, Huber R, Wiegand G, J Biol Chem, 2003
Rational design of a CD4 mimic that inhibits HIV-1 entry and exposes cryptic neutralization epitopes
Martin L, Stricher F, Misse D, Sironi F, Pugniere M, Barthe P, Prado-Gotor R, Freulon I, Magne X, Roumestand C, Menez A, Lusso P, Veas F, Vita C, Nat Biotechnol, 2003
2002
Equilibrium and pressure-jump relaxation studies of the conformational transitions of P13MTCP1
Kitahara R, Royer C, Yamada H, Boyer M, Saldana JL, Akasaka K, Roumestand C, J Mol Biol, 2002
Solution structure of the LicT-RNA antitermination complex: CAT clamping RAT
Yang Y, Declerck N, Manival X, Aymerich S, Kochoyan M, Embo J, 2002
Identification of Akt association and oligomerization domains of the Akt kinase coactivator TCL1.
Kunstle G, Laine J, Pierron G, Kagami SS, Nakajima H, Hoh F, Roumestand C, Stern MH, M. N, Mol Cell Biol, 2002
1H, 15N and 13C backbone resonance assignments of the 40 kDa LicT-CAT-PRD1 protein
Ducat T, Declerck N, Kochoyan M, Demene H, J Biomol NMR, 2002
Structure refinement of flexible proteins using dipolar couplings: application to the protein p8MTCP1
Demene H, Ducat T, Barthe P, Delsuc MA, Roumestand C, J. Biomol. NMR., 2002
RNA recognition by transcriptional antiterminators of the BglG/SacY family: mapping of SacY RNA binding site
Declerck N, Minh NL, Yang Y, Bloch V, Kochoyan M, Aymerich S, J Mol Biol, 2002
Helix motion in protein C12A-p8(MTCP1): comparison of molecular dynamics simulations and multifield NMR relaxation data
Barthe P, Roumestand C, Demene H, Chiche L, J. Comput. Chem., 2002
2001
A comprehensive analysis of multifield 15N relaxation parameters in proteins: determination of 15N chemical shift anisotropies
Canet D, Barthe P, Mutzenhardt P, Roumestand C, J. Am. Chem. Soc., 2001
Dimer stabilization upon activation of the transcriptional antiterminator LicT
Declerck N, Dutartre H, Receveur V, Dubois V, Royer C, Aymerich S, van Tilbeurgh H, J Mol Biol, 2001
The use of sample rotation for minimizing convection effects in self- diffusion NMR measurements
Esturau N, Sanchez-Ferrando F, Gavin JA, Roumestand C, Delsuc MA, Parella T, J Magn Reson, 2001
FIRE: predicting the spatial proximity of protein residues from 3D NOESY-HSQC
Malliavin T, Barthe P, Delsuc MA, Theor. Chem. Acc., 2001
Characterization of the folding and unfolding reactions of a small beta-barrel protein of novel topology, the MTCP1 oncogene product P13
Roumestand C, Boyer M, Guignard L, Barthe P, Royer CA, J. Mol. Biol., 2001
Sites of positive and negative regulation in the Bacillus subtilis antiterminators LicT and SacY
Tortosa P, Declerck N, Dutartre H, Lindner C, Deutscher J, Le Coq D, Mol Microbiol, 2001
Structural insights into the regulation of bacterial signalling proteins containing PRDs
van Tilbeurgh H, Declerck N, Curr Opin Struct Biol, 2001
Crystal structure of an activated form of the PTS regulation domain from the LicT transcriptional antiterminator
van Tilbeurgh H, Le Coq D, Declerck N, Embo J, 2001
2000
Engineering novel bioactive mini-proteins on natural scaffolds
Martin L, Barthe P, Combes O, Roumestand C, Vita C, Tetrahedron, 2000
Extending the excitation sculpting concept for selective excitation
Roumestand C, Canet D, J Magn Reson, 2000
Synthesis and NMR solution structure of an alpha-helical hairpin stapled with two disulfide bridges
Barthe P, Rochette S, Vita C, Roumestand C, Protein Sci., 2000
Backbone dynamics and solution structure refinement of the 15N-labeled human oncogenic protein p13MTCP1: comparison with X-ray data
Guignard L, Padilla A, Mispelter J, Yang YS, Stern MH, Lhoste JM, Roumestand C, J Biomol NMR, 2000
1999
Backbone dynamics study of C12A-P8MTCP1 using the linear correlation approach at 2 magnetic field strength. Effect of protein aggregation
Bartes P, N.Declerck, Delsuc M-A, Lefevre J-F, Roumestand C, J.Chim.Phys., 1999
Refined solution structure and backbone dynamics of 15N-labeled C12A-p8MTCP1 studied by NMR relaxation
Barthe P, Chiche L, Declerck N, Delsuc MA, Lefevre JF, Malliavin T, Mispelter J, Stern MH, Lhoste JM, Roumestand C, J. Biomol. NMR., 1999
Backbone dynamics study of C12A-p8MTCP1 using the linear correlation approach at two magnetic field strengths. Effect of protein aggregation.
Barthe P, Declerck N, Delsuc MA, Lefevre JF, Roumestand C, J. Chim. Phys., 1999
Characterization and molecular cloning of an adenosine kinase from Babesia canis rossi
Carret C, Delbecq S, Labesse G, Carcy B, Precigout E, Moubri K, Schetters TP, Gorenflot A, Eur J Biochem, 1999
RNA recognition by transcriptional antiterminators of the BglG/SacY family: functional and structural comparison of the CAT domain from SacY and LicT
Declerck N, Vincent F, Hoh F, Aymerich S, van Tilbeurgh H, J Mol Biol, 1999
Two well-defined motifs in the cAMP-dependent protein kinase inhibitor (PKIalpha) correlate with inhibitory and nuclear export function
Hauer JA, Barthe P, Taylor SS, Parello J, Padilla A, Protein Sci., 1999
A practical approach to the implementation of selectivity in homonuclear multidimensional NMR with frequency selective-filtering techniques. Application to the chemical structure elucidation of complex oligosaccharides.
Roumestand C, Delay C, Gavin JA, Canet D, Magn. Reson. Chem., 1999
Rational engineering of a miniprotein that reproduces the core of the CD4 site interacting with HIV-1 envelope glycoprotein
Vita C, Drakopoulou E, Vizzavona J, Rochette S, Martin L, Menez A, Roumestand C, Yang YS, Ylisastigui L, Benjouad A, Gluckman JC, Proc Natl Acad Sci U S A, 1999
1998
Solution structure of the recombinant human oncoprotein p13MTCP1
Yang YS, Guignard L, Padilla A, Hoh F, Strub MP, Stern MH, Lhoste JM, Roumestand C, J Biomol NMR, 1998
Oligomerization of protegrin-1 in the presence of DPC micelles. A proton high-resolution NMR study
Roumestand C, Louis V, Aumelas A, Grassy G, Calas B, Chavanieu A, FEBS Lett, 1998
The preliminary solution structure of human p8MTCP1, a protein encoded by the putative MTCP1 oncogene
Barthe P, Guignard L, Yang YS, Chiche L, Strub MP, Hoh F, Stern MH, Roumestand C, J. Chim. Phys., 1998
Formation of native disulfide bonds in endothelin-1. Structural evidence for the involvement of a highly specific salt bridge between the prosequence and the endothelin-1 sequence
Aumelas A, Kubo S, Chino N, Chiche L, Forest E, Roumestand C, Kobayashi Y, Biochemistry, 1998
1997
Solution structure of human p8MTCP1, a cysteine-rich protein encoded by the MTCP1 oncogene, reveals a new alpha-helical assembly motif
Barthe P, Yang YS, Chiche L, Hoh F, Strub MP, Guignard L, Soulier J, Stern MH, van Tilbeurgh H, Lhoste JM, Roumestand C, J. Mol. Biol., 1997
On the convergent evolution of animal toxins. Conservation of a diad of functional residues in potassium channel-blocking toxins with unrelated structures
Dauplais M, Lecoq A, Song J, Cotton J, Jamin N, Gilquin B, Roumestand C, Vita C, de Medeiros CL, Rowan EG, Harvey AL, Menez A, J Biol Chem, 1997
Isolation and structure elucidation of a highly haemolytic saponin from the Merck saponin extract using high-field gradient-enhanced NMR techniques
Delay C, Gavin JA, Aumelas A, Bonnet PA, Roumestand C, Carbohydr Res, 1997
1996
Synthesis and solution structure of the antimicrobial peptide protegrin-1
Aumelas A, Mangoni M, Roumestand C, Chiche L, Despaux E, Grassy G, Calas B, Chavanieu A, Eur J Biochem, 1996
Change in membrane permeability induced by protegrin 1: implication of disulphide bridges for pore formation
Mangoni ME, Aumelas A, Charnet P, Roumestand C, Chiche L, Despaux E, Grassy G, Calas B, Chavanieu A, FEBS Lett, 1996
Gradient-Enhanced Band-Filtering Experiments.
Roumestand C, Mutzenhardt P, Delay C, Canet D, Magn. Reson. Chem., 1996
| Director | Emmanuel Margeat |
| Deputy directors | Vanessa Delfosse Martin Cohen-Gonsaud |
| Admin.Resp. | Florence Lepage |
| (33) 04 67 41 79 01 |
| Manager.Assist. | Marion Boutin |
| (33) 04 67 41 79 04 |
