Centre de Biologie Structurale

 
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Seminars

  • 13 May
    LLPS Meeting 13 May 2024 11:00 AM to 12:00 PM

  • 16 May
    "Ask the ancestors: resurrecting and re-evolving the bacterial flagellar motor" Matthew Baker, University New South Wales, Sydney 16 May 2024 11:00 AM to 12:00 PM

    Matthew Baker,Scientia Associate Professor, UNSW Sydney (BABS), Faculty of Science.Visiting Professor, Institute of Molecular Biology and Biotechnology

  • 17 May
    Young CBS Researchers Meeting 17 May 2024 04:30 PM to 05:30 PM

  • 13 May - 17 May
  • 23 May - 27 May
  • 31 May - 06 Jun
  • 10 Jun - 14 Jun
  • 17 Jun - 24 Jun
  • 27 Jun - 01 Jul
  • 02 Jul - 05 Jul
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SmFRET TCSPC

Equipement

SmFRET TCSPC

Measure

Measure conformational changes, interactions, changes in the environment of single molecules.

Confocal-based smFRET, FCS, FCCS, PIE, TCSPC, MFD
 
A portion of the TCSPC smFRET light path		  
Specifications Pulsed white supercontinuum source (100ps / 50ns) split in two optical paths, one of them is delayed (7m => 21ns delay)
Band-pass filters to select excitation wavelengths (488, 532, 590, 633, 740nm).
Prompt and delayed beams are recombined in a single mode optical fiber (to clean the beam + ensure proper overlay of all wavelengths)
Pulse synchronizer on the prompt path APM-400, B&H
Nikon statif with perfect focus system (cannot be used at 740nm excitation).
Objectives: 100X NA 1 .4 oil

Sample motorized stage (MS-2000, ASI)
Dual polarisation + dual color emission splitter => 2+2 APD (MFD detection)
TCSPC counting board (SPC-150, Becker&Hickl) + 4 channel router
Comment For full description, please refer to: Olofsson et al, Opt Expr (2013)
https://www.osapublishing.org/oe/abstract.cfm?uri=oe-21-3-3370
Room Z2-13@CBS29

Synergy H1

Equipement

Synergy H1

Measure

Fluorescence, polarization, luminescence measurements
  • Fluorescence reader for microplates
  • UV-Vis absorbance
  • Fluorescence intensity
  • Luminescence
  • Fluorescence polarization
  • Time-resolved fluorescence
 
Synergy H1		  
Specifications
  • Variable filters
  • Endpoint, or kinetic measurements
  • Spectral scanning, well area scanning
  • 6- to 384-well plates
Comment Essentially used to run long kinetics (O/N)
Get more information @ the manufacturer’s website:
https://www.biotek.com/products/detection-hybrid-technology-multi-mode-microplate-readers/synergy-h1-hybrid-multi-mode-reader/overview/
Room Z2-7@CBS29

TIRF / 3D MFM

Equipement

TIRF / 3D MFM

Measure

Measure conformational changes, interactions, changes in the environment of single molecules in milliseconds to seconds timescale. Measure fast (milliseconds timescale) two and three dimensional localization of molecules of interest.

TIRF with dual-view on one side => Single particle tracking, single molecule FRET with Alex ( Alternating-laser excitation)
3D-MFM on the other side => Multi-focal microscopy (3D imaging and tracking)
Specifications TIRF/HILO/Epifluorescence wide field configuration. Dual color smFRET/SPT and 3D imaging and tracking
Microscope:
  • Inverted microscope (Zeiss Axiovert 200)
  • Zeiss alpha Plan-Apochromat, 100X, NA1.46
  • Band-pass filters to select excitation wavelengths (450, 488, 514, 532, 640 nm)
Lasers:
  • Laser diodes 450, 488, 525 nm, Thorlabs PL450B, L488P60 and PL520
  • Laser 532nm,
  • Laser 640 nm, spectra physics, EXLR-640C-100-CDRH
All laser lines are controlled through an AOTF (AOTFnC-400.650-TN AAoptics, France) and can be set to TIRF/HILO/Epifluorescence illumination mode
MFM setup:
  • First relay lense L1 (f = 150 mm(to form a secondary Fourier plane, conjugate of the objective pupil plane)
  • Custom-built multifocus grating (MFG)
  • Chromatic correction grating (CCG)
  • Multifacet prism, combined with a second relay lens L2 (f = 200mm)
  • emCCD camera (IXON DU-897, 512*512, 16µm, Andor, Ireland)
optional : cylindrical lens in front of the em-CCD (for 3D single molecule localization using astigmatism).
Room X.room

TECAN Safire 2

Equipement

TECAN Safire2

Measure

Polarization / anisotropy

Microplate reader for polarization / anisotropy to measure molecular interactions.
 
TECAN Safire2		  
Specifications
  • Fluorescence reader for microplates
  • UV-Vis absorbance
  • Fluorescence intensity
  • Luminescence
  • Fluorescence polarization
Temperature control (heating only)
Shaking
Endpoint or kinetic spectral measurements
Possibility to run excitation / emission spectra matrices
Absorption / Fluorescence:
Double monochromators in excitation and emission
  • bandwidth of 10nm in UV, 20nm in visible light
  • wavelength from 230nm to 850nm
Polarization:
  • 4 LEDs : 488, 532, 594, 633nm
  • double monochromators in detection
Comment The absorption / fluorescence lamp decays in intensity and cannot be replaced => it is better to use the CLARIOstar for these applications.
Polarization detection is highly sensitive.
Room Z2-7@CBS29

Typhoon FLA 9500

Equipement

Typhoon FLA 9500

Measure

Fluorescence scanner for gels

Laser scanner for fluorescence imaging => precise quantitation of proteins, nucleic acids, and other biomolecules. Fluorescence detection on gels, membranes, petri dishes, multi-well plates…
 
Typhoon FLA9500		  
Specifications
  • High resolution and quantitation: a pixel resolution of up to 10 µm and a linear signal response over five orders of magnitude provides precise quantitation in gels, blots, tissue sections, and arrays
  • High sample throughput: scanning area of 40 × 46 cm enables simultaneous imaging of up to 20 mini-gels or blots
  • 3 lasers : 473, 532, 635nm + 4 emission filters :
  • BPB1 (520-540nm)
  • BPG1 (560-580nm)
  • LPG (≥ 575nm)
  • LPR (≥ 665nm)
Comment Get more information @ the manufacturer website:
https://www.gelifesciences.com/en/us/shop/typhoon-fla-9500-p-00190
Room Z2-7@CBS29

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