| Equipement |
VP ITC Microcalorimetry |
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| Measure |
Determination of affinity constants in protein/protein or protein/ligand or protein/DNA interactions. Determination of the stoichiometry of the biochemical interaction. Determination of thermodynamic constants such the enthalpy and the entropy. |
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Sample volume 2 ml at around 10-20 uM for the protein, 500 ul at 100-200 uM for the ligand. Duration 3 hours. |
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 Isothermal titration calorimeter VP-ITC MicroCal (Malvern InstrumentsTM) |
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| Specifications | https://www.malvernpanalytical.com/fr/support/product-support/microcal-range/microcal-itc-range/microcal-vp-itc | |
| Room | Z2-16@CBS29 | |