| Equipement |
SmFRET TCSPC
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| Measure |
Measure conformational changes, interactions, changes in the environment of single molecules. |
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Confocal-based smFRET, FCS, FCCS, PIE, TCSPC, MFD |
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 A portion of the TCSPC smFRET light path |
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| Specifications | Pulsed white supercontinuum source (100ps / 50ns) split in two optical paths, one of them is delayed (7m => 21ns delay) Band-pass filters to select excitation wavelengths (488, 532, 590, 633, 740nm). Prompt and delayed beams are recombined in a single mode optical fiber (to clean the beam + ensure proper overlay of all wavelengths) Pulse synchronizer on the prompt path APM-400, B&H Nikon statif with perfect focus system (cannot be used at 740nm excitation). Objectives: 100X NA 1 .4 oil Sample motorized stage (MS-2000, ASI) Dual polarisation + dual color emission splitter => 2+2 APD (MFD detection) TCSPC counting board (SPC-150, Becker&Hickl) + 4 channel router |
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| Comment | For full description, please refer to: Olofsson et al, Opt Expr (2013) https://www.osapublishing.org/oe/abstract.cfm?uri=oe-21-3-3370 |
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| Room | Z2-13@CBS29 | |