instrument

VP ITC Microcalorimetry

*Equipement*	VP ITC Microcalorimetry
*Measure*	Determination of affinity constants in protein/protein or protein/ligand or protein/DNA interactions. Determination of the stoichiometry of the biochemical interaction. Determination of thermodynamic constants such the enthalpy and the entropy.
Sample volume 2 ml at around 10-20 uM for the protein, 500 ul at 100-200 uM for the ligand. Duration 3 hours.
	Isothermal titration calorimeter VP-ITC MicroCal (Malvern InstrumentsTM)
*Specifications*	https://www.malvernpanalytical.com/fr/support/product-support/microcal-range/microcal-itc-range/microcal-vp-itc
*Room*	Z2-16@CBS29

SEC-MALS

*Equipement*	SEC- MALS
*Measure*	Determination of molecular weight of proteins, protein/protein complexes, proteins/nucleic acids, nanodisks…Determination of protein oligomerization state and stoichiometry of complexes.
Sample volume 20 ul at 2 mg/ml, duration 1 hours. Different size exclusion columns available.
	SEC –MALS : HPLC (AgilentTM Infinity II), Detector LS (miniDAWN TREOS – WayttTM), refractometry (Optilab T-rEX – WayttTM).
*Specifications*	https://www.wyatt.com/products/instruments/minidawn-multi-angle-light-scattering-detector-sec-mals.html https://www.wyatt.com/products/instruments/optilab-refractive-index-detector.html
*Room*	Z1-5@CBS29

Dynamic Light Scattering (DLS)

*Equipement*	Dynamic Light Scattering (DLS)
*Measure*	Molecule hydrodynamic radius measurement.
Measurement in microcuvettes. Reaction volume 40 ul with a final protein concentration around 100 uM. Duration 15 min.
	DLS Zetasizer Nano-S (Malvern Instrument TM)
*Specifications*	https://www.malvernpanalytical.com/fr/products/product-range/zetasizer-range/zetasizer-nano-range/zetasizer-nano-s
*Room*	Z2-16@CBS29

Nano DSF

*Equipement*	Nano DSF
*Measure*	Tm measurement
Simultaneous measurements of 6 different proteins or conditions. Sample volume 10 ul. Duration 3 min.
	Nanotemper™ Tycho NT.6
*Specifications*	https://nanotempertech.com/tycho/
*Room*	Z2-16@CBS29

Circular Dichroism

*Equipement*	Circular Dichroism
*Measure*	Respective proportion estimation of the different secondary structure elements present in a protein.
Precision cells; light path 0,1 - 0,2 - 0,5 - 1 - 2 - 5 - 10 mm. Final protein concentration around 50-100 uM. Duration 10 min.
	CD Chirascan (Applied Biophysics TM)
*Specifications*	https://www.photophysics.com/systems/chirascan-systems/chirascan/system-information/
*Room*	Z2-16@CBS29

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