MARS Biophotonics - R&D division

The main objective of MARS is to provide the scientific community with access to a selection of advanced microscopy technologies, generally not commercially available. In order to keep up with the latest developments in the microscopy community, we are continuously implementing state-of-the-art optical microscopy methods developed by the R&D teams of the FBI node hosted at CBS (Nollmann, Margeat, Milhiet teams). At the moment, a 3D STED setup, a Multi-Focus Microscope and a light-sheet microscope are under construction on our R&D facility. Stay tuned!

As our instruments are homemade, we also have the flexibility to adapt our current setups and analysis softwares to the specific needs of users (see examples below where instruments and analyses have been adapted to biological systems).

User requests are evaluated by the MARS Executive Committee according to project feasibility, and availability of engineers and instruments.

Examples of success stories:

Martiniere A, Fiche JB, Smokvarska M, Mari S, Alcon C, Dumont X, Hematy K, Jaillais Y, Nollmann M, Maurel C Osmotic stress activates two reactive oxygen species pathways with distinct effects on protein nanodomains and diffusion Plant Physiol 2019, 179 ( 4): 1581-1593 pubmed Id 30718348

Platre MP, Bayle V, Armengot L, Bareille J, Marques-Bueno MDM, Creff A, Maneta-Peyret L, Fiche JB, Nollmann M, Miege C, Moreau P, Martiniere A, Jaillais Y Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine Science 2019,364(6435):57-62 pubmed Id 30948546

Møller T.C., Hottin J., Clerté C., Zwier J.M., Durroux T., Rondard P., Prezeau L., Royer C.A., Pin J.P., Margeat E., Kniazeff J. Oligomerization of a metabotropic glutamate receptor in neurons controlled by its structural dynamics, Scientifics Reports, 2018

Faure LM, Fiche JB, Espinosa L, Ducret A, Anantharaman V, Luciano J, Lhospice S, Islam ST, Treguier J, Sotes M, Kuru E, Van Nieuwenhze MS, Brun YV, Theodoly O, L A, Nollmann M, Mignot T The mechanism of force transmission at bacterial focal adhesion complexes Nature 2016, 539(7630):530-535 pubmed Id 27749817

Ferrer M, Clerte C, Chamontin C, Basyuk E, Laine S, Hottin J, Bertrand E, Margeat E, Mougel M. Imaging HIV-1 RNA dimerization in cells by multicolor super-resolution and fluctuation microscopies, Nucleic Acids Res, 2016