| Equipement |
PALM
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| Measure |
Single Molecule Localization Microscope |
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PALM, dSTORM, SPT-PALM, TIRF |
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| Specifications | Custom-made inverted microscope (Zeiss stage) objective: Plan-Apocromat, 100x, 1.4NA oil DIC (Zeiss) z-axis piezoelectric stage (P-721.CDQ, PICOF, PI). 1.5x telescope (pixel size ≈105 nm) Lasers:
Emission filters: ET525/50 m, ET600/50 m and ET700/75m (Chroma Technology) EMCCD camera (iXon X3 DU-897, Andor Technologies) Sample motorized stage Home-made auto- focus Camera, lasers and filter wheel are controlled with software written in Labview. |
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| Comment | For full description, please refer to: Georgieva et al, Methods (2016) https://www.sciencedirect.com/science/article/pii/S1046202316300639 |
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| Room | @MARS | |