| Equipement |
2 photons FCS
|
| Measure |
Measure diffusion, concentration, brightness or oligomerization state of fluorescently labeled molecules in vitro or in vivo.
|
|
FCS, FCCS, Number and Brightness, RICS…
Simultaneous acquisition in 2 emission channels
|
| |
2 photons FCS @CBS29 |
|
| Specifications |
- Mai Tai Laser 700-1020nm. 2 photons excitation
- ISS fast laser scanning module
- Detection on 2 low dark counts APDs with 5 different dichroic and filters sets to detect fluorescence from classical dyes (CFP, GFP, mCherry, Cy5…).
- Epi-illumination lamp
- Nikon statif + 60X NA 1.4 oil
60X NA 1.2 water (=> deeper in the sample + less chromatic aberrations)
- Z-piezo on the objective
- No auto-focus system.
- Different sample holders
- No heating system, no CO2 control.
|
| Room |
Z2-17@CBS29 |
