Plateforme Intégrée de Biophysique et de Biologie Structurale


2 photons FCS


Measure diffusion, concentration, brightness or oligomerization state of fluorescently labeled molecules in vitro or in vivo.

FCS, FCCS, Number and Brightness, RICS…
Simultaneous acquisition in 2 emission channels

2 photons FCS @CBS29
  • Mai Tai Laser 700-1020nm. 2 photons excitation
  • ISS fast laser scanning module
  • Detection on 2 low dark counts APDs with 5 different dichroic and filters sets to detect fluorescence from classical dyes (CFP, GFP, mCherry, Cy5…).
  • Epi-illumination lamp
  • Nikon statif + 60X NA 1.4 oil
60X NA 1.2 water (=> deeper in the sample + less chromatic aberrations)
  • Z-piezo on the objective
  • No auto-focus system.
  • Different sample holders
  • No heating system, no CO2 control.
Room Z2-17@CBS29