2 photons FCS

Measure diffusion, concentration, brightness or oligomerization state of fluorescently labeled molecules in vitro or in vivo.

FCS, FCCS, Number and Brightness, RICS…
Simultaneous acquisition in 2 emission channels

• Mai Tai Laser 700-1020nm. 2 photons excitation
• ISS fast laser scanning module
• Detection on 2 low dark counts APDs with 5 different dichroic and filters sets to detect fluorescence from classical dyes (CFP, GFP, mCherry, Cy5…).
• Epi-illumination lamp
• Nikon statif + 60X NA 1.4 oil

• Z-piezo on the objective
• No auto-focus system.
• Different sample holders
• No heating system, no CO2 control.