Multimode equipped with Nanoscope VIII (BRUKER)

Characterize biological samples topography at nanoscale in dry or liquid environment. Image acquisition can be performed in static or dynamic modes.

The technique allows characterizing sample morphology at nanoscale. Images are acquired by scanning a nanometric tip in gentle contact or intermittent contact with the sample. The tip is positioned at the end of a micrometric force transducer, the AFM cantilever: it records the variation of sample topography due to changes of tip-sample interaction during scanning operation. In addition, by measuring the tip-sample interaction force as a function of the tip-sample distance, AFMs can evaluate sample elastic and viscous properties.