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Publications
2026
Recent advances on structure and dynamics of G protein-coupled receptors using single-molecule Foerster resonance energy transfer
Quast RB, Margeat E, Curr Opin Struct Biol, 2026
Challenging cases for AlphaFold: two multidomain proteins with zinc-binding-, phosphorylation- or dimerization-driven conformational changes
Yang Y, Demene H, Eur Biophys J, 2026
Distinct Spatial Organisation of Rho and RNA Polymerase in Salmonella Cells
Bossi L, Le Bars R, Black JC, Buggiani J, Clerte C, Do TD, Margeat E, Boudvillain M, Figueroa-Bossi N, Mol Microbiol, 2026
Unveiling the Impact of Drug-Sensitive Mutations on HIV-1 Protease Dynamics: A Molecular Dynamics Simulation Study of the T12A, L63Q, and H69N Variants
Srihi H, Abid N, Fabeni L, Precone C, Demene H, Chillemi G, Int J Mol Sci, 2026
Statistical models to characterize colon tumor stiffness heterogeneity through representative atomic force microscopy maps
Gadouas G, Tosato G, Costa L, Thierry AR, Ravel P, Mazard T, Milhiet PE, Benistant C, Lopez-Crapez E, Colinge J, Sci Rep, 2026
2025
Ultra-Fast Isothermal Formation of DNA Nanostructures in Culture Media: Application to In Situ Assembly of DNA Origami With Living Cells
Bourdon L, Wilkens GD, Dehissi S, Hotte S, Rudiuk S, Morel M, Yamada A, Bellot G, Baigl D, Small, 2025
An innovative in situAFM system for a soft X-ray spectromicroscopy synchrotron beamline
Hafner A, Costa L, Kourousias G, Bonanni V, Zizic M, Stolfa A, Bazi B, Vincze L, Gianoncelli A, Analyst, 2025
Imaging Nuclear Envelopes Using Correlative AFM/Fluorescence Microscopy
Costes E, Vial A, Doucet C, Milhiet PE, Methods Mol Biol, 2025
Automated identification of honey bee pollen loads for field-applied palynological studies
Bares J, Poncelet P, Doucet CM, Legrand C, Cambon A, Carlier C, Chevin P, Dewaele LP, Jullien D, Thibaud JB, Charnet P, Rousset M, Cheptou PO, New Phytol, 2025
Picomolar bivalent inhibitors of protein kinase CK2 active against beta-coronavirus replication
Grenier D, Lopez Molina DS, Gelin M, Mularoni A, Guichou JF, Delcros JG, Martinasso C, Yang Y, Ahnou N, Pawlotsky JM, Ahmed-Belkacem A, Krimm I, Eur J Med Chem, 2025
Rational Design and Synthesis of Highly Stable Haloflavanone DNA Methyltransferase Inhibitors Inducing Tumor Suppressor Gene Re-expression in Cancer Cells
Calzaferri F, Daher H, Gilbert J, Yang Y, Tauziet M, Jallet C, Bessin Y, van der Lee A, Arimondo PB, Krimm I, Julien E, Lopez M, J Med Chem, 2025
Triple Labeling Resolves a GPCR Intermediate State by Using Three-Color Single Molecule FRET
Bonhomme L, Bilgen E, Clerte C, Pin JP, Rondard P, Margeat E, Lamb DC, Quast RB, J Am Chem Soc, 2025
Single-stranded DNA drives sigma subunit loading onto mycobacterial RNA polymerase to unlock initiation-competent conformations
Vishwakarma RK, Marechal N, Morichaud Z, Blaise M, Margeat E, Brodolin K, Nucleic Acids Res, 2025
Binding-Site Switch for Protein Kinase CK2 Inhibitors
Grenier D, Gelin M, Yang Y, Mularoni A, Guichou JF, Delcros JG, Krimm I, ChemMedChem, 2025
2024
Structural basis of orientated asymmetry in a mGlu heterodimer
Huang W, Jin N, Guo J, Shen C, Xu C, Xi K, Bonhomme L, Quast RB, Shen DD, Qin J, Liu YR, Song Y, Gao Y, Margeat E, Rondard P, Pin JP, Zhang Y, Liu J, Nat Commun, 2024
Biased activation of the vasopressin V2 receptor probed by molecular dynamics simulations, NMR and pharmacological studies
Fouillen A, Couvineau P, Gaibelet G, Riche S, Orcel H, Mendre C, Kanso A, Lanotte R, Nguyen J, Dimon J, Urbach S, Sounier R, Granier S, Bonnet D, Cong X, Mouillac B, Demene H, Comput Struct Biotechnol J, 2024
Split Reporters Facilitate Monitoring of Gene Expression and Peptide Production in Linear Cell-Free Transcription-Translation Systems
Levrier A, Capin J, Mayonove P, Karpathakis II, Voyvodic P, DeVisch A, Zuniga A, Cohen-Gonsaud M, Cabantous S, Noireaux V, Bonnet J, ACS Synth Biol, 2024
Quantifying surface tension and viscosity in biomolecular condensates by FRAP-ID
Santamaria A, Hutin S, Doucet CM, Zubieta C, Milhiet PE, Costa L, Biophys J, 2024
Free-Standing DNA Origami Superlattice to Facilitate Cryo-EM Visualization of Membrane Vesicles
Aissaoui N, Mills A, Lai-Kee-Him J, Triomphe N, Cece Q, Doucet C, Bonhoure A, Vidal M, Ke Y, Bellot G, J Am Chem Soc, 2024
Critical micellar concentration determination of pure phospholipids and lipid-raft and their mixtures with cholesterol
Serravalle S, Pisano M, Sciacca MFM, Salamone N, Sicali L, Mazzara G, Costa L, La Rosa C, Proteins, 2024
2023
Isothermal self-assembly of multicomponent and evolutive DNA nanostructures
Rossi-Gendron C, El Fakih F, Bourdon L, Nakazawa K, Finkel J, Triomphe N, Chocron L, Endo M, Sugiyama H, Bellot G, Morel M, Rudiuk S, Baigl D, Nat Nanotechnol, 2023
Phase separation and molecular ordering of the prion-like domain of the Arabidopsis thermosensory protein EARLY FLOWERING 3
Hutin S, Kumita JR, Strotmann VI, Dolata A, Ling WL, Louafi N, Popov A, Milhiet PE, Blackledge M, Nanao MH, Wigge PA, Stahl Y, Costa L, Tully MD, Zubieta C, Proc Natl Acad Sci U S A, 2023
Concerted conformational changes control metabotropic glutamate receptor activity
Lecat-Guillet N, Quast RB, Liu H, Bourrier E, Moller TC, Rovira X, Soldevila S, Lamarque L, Trinquet E, Liu J, Pin JP, Rondard P, Margeat E, Sci Adv, 2023
Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae
Hertzog M, Perry TN, Dupaigne P, Serres S, Morales V, Soulet AL, Bell JC, Margeat E, Kowalczykowski SC, Le Cam E, Fronzes R, Polard P, Nucleic Acids Res, 2023
A Tripartite Complex HIV-1 Tat-Cyclophilin A-Capsid Protein Enables Tat Encapsidation That Is Required for HIV-1 Infectivity
Schatz M, Marty L, Ounadjela C, Tong PBV, Cardace I, Mettling C, Milhiet PE, Costa L, Godefroy C, Pugniere M, Guichou JF, Mesnard JM, Blaise M, Beaumelle B, J Virol, 2023
Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins
Agam G, Gebhardt C, Popara M, Machtel R, Folz J, Ambrose B, Chamachi N, Chung SY, Craggs TD, de Boer M, Grohmann D, Ha T, Hartmann A, Hendrix J, Hirschfeld V, Hubner CG, Hugel T, Kammerer D, Kang HS, Kapanidis AN, Krainer G, Kramm K, Lemke EA, Lerner E, Margeat E, Martens K, Michaelis J, Mitra J, Moya Munoz GG, Quast RB, Robb NC, Sattler M, Schlierf M, Schneider J, Schroeder T, Sefer A, Tan PS, Thurn J, Tinnefeld P, van Noort J, Weiss S, Wendler N, Zijlstra N, Barth A, Seidel CAM, Lamb DC, Cordes T, Nat Methods, 2023
Structure and mechanics of the human nuclear pore complex basket using correlative AFM-fluorescence superresolution microscopy
Vial A, Costa L, Dosset P, Rosso P, Boutieres G, Faklaris O, Haschke H, Milhiet PE, Doucet CM, Nanoscale, 2023
The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity
Elena-Real CA, Sagar A, Urbanek A, Popovic M, Morato A, Estana A, Fournet A, Doucet C, Lund XL, Shi ZD, Costa L, Thureau A, Allemand F, Swenson RE, Milhiet PE, Crehuet R, Barducci A, Cortes J, Sinnaeve D, Sibille N, Bernado P, Nat Struct Mol Biol, 2023
2022
Mechanical DNA Origami to Investigate Biological Systems
Mills A, Aissaoui N, Finkel J, Elezgaray J, Bellot G, Adv Biol (Weinh), 2022
Magneto-Induced Hyperthermia and Temperature Detection in Single Iron Oxide Core-Silica/Tb3+/Eu3+(Acac) Shell Nano-Objects
Nigoghossian K, Bouvet B, Felix G, Sene S, Costa L, Milhet PE, Carneiro Neto AN, Carlos LD, Oliviero E, Guari Y, Larionova J, Nanomaterials (Basel), 2022
Viro-fluidics: Real-time analysis of virus production kinetics at the single-cell level
Eid J, Socol M, Naillon A, Feuillard J, Ciandrini L, Margeat E, Charlot B, Mougel M, Biophys Rep (N Y), 2022
A modular spring-loaded actuator for mechanical activation of membrane proteins
Mills A, Aissaoui N, Maurel D, Elezgaray J, Morvan F, Vasseur JJ, Margeat E, Quast RB, Lai-Kee-Him J, Saint N, Benistant C, Nord A, Pedaci F, Bellot G, Nat Commun, 2022
Mechanical signatures of human colon cancers
Lopez-Crapez E, Costa L, Tosato G, Ramos J, Mazard T, Guiramand J, Thierry A, Colinge J, Milhiet PE, Benistant C, Sci Rep, 2022
A flexible and original architecture of two unrelated zinc fingers underlies the role of the multitask P1 in RYMV spread
Poignavent V, Hoh F, Terral G, Yang Y, Gillet FX, Kim JH, Allemand F, Lacombe E, Brugidou C, Cianferani S, Demene H, Vignols F, J Mol Biol, 2022
Agonists and allosteric modulators promote signaling from different metabotropic glutamate receptor 5 conformations
Nasrallah C, Cannone G, Briot J, Rottier K, Berizzi AE, Huang CY, Quast RB, Hoh F, Baneres JL, Malhaire F, Berto L, Dumazer A, Font-Ingles J, Gomez-Santacana X, Catena J, Kniazeff J, Goudet C, Llebaria A, Pin JP, Vinothkumar KR, Lebon G, Cell Rep, 2022
Cryo-EM structure of transcription termination factor Rho from Mycobacterium tuberculosis reveals bicyclomycin resistance mechanism
Saridakis E, Vishwakarma R, Lai-Kee-Him J, Martin K, Simon I, Cohen-Gonsaud M, Coste F, Bron P, Margeat E, Boudvillain M, Commun Biol, 2022
Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts
Batista AC, Levrier A, Soudier P, Voyvodic PL, Achmedov T, Reif-Trauttmansdorff T, DeVisch A, Cohen-Gonsaud M, Faulon JL, Beisel CL, Bonnet J, Kushwaha M, ACS Synth Biol, 2022
2021
Melanotransferrin is efficiently sorted on the surface of exosomes secreted by melanoma cells
Bonhoure A, Henry L, Morille M, Aissaoui N, Bellot G, Stoebner PE, Vidal M, Melanoma Res, 2021
Allosteric modulators enhance agonist efficacy by increasing the residence time of a GPCR in the active state
Cao AM, Quast RB, Fatemi F, Rondard P, Pin JP, Margeat E, Nat Commun, 2021
Molecular insights into the biased signaling mechanism of the mu-opioid receptor
Cong X, Maurel D, Demene H, Vasiliauskaite-Brooks I, Hagelberger J, Peysson F, Saint-Paul J, Golebiowski J, Granier S, Sounier R, Mol Cell, 2021
CD82 and Gangliosides Tune CD81 Membrane Behavior
Fernandez L, Malrieu M, Benistant C, Dosset P, Rubinstein E, Odintsova E, Berditchevski F, Milhiet PE, Int J Mol Sci, 2021
Comparative Assessment of NMR Probes for the Experimental Description of Protein Folding Pathways with High-Pressure NMR
Van Deuren V, Yang YS, deGuillen K, Dubois C, Royer CA, Roumestand C, Barthe P, Biology (Basel), 2021
Correlative AFM and fluorescence imaging demonstrate nanoscale membrane remodeling and ring-like and tubular structure formation by septins
Vial A, Taveneau C, Costa L, Chauvin B, Nasrallah H, Godefroy C, Dosset P, Isambert H, Ngo KX, Mangenot S, Levy D, Bertin A, Milhiet PE, Nanoscale, 2021
A Novel Approach to the Facile Growth and Organization of Photothermal Prussian Blue Nanocrystals on Different Surfaces
Ngo G, Felix G, Dorandeu C, Devoisselle JM, Costa L, Milhiet PE, Guari Y, Larionova J, Chopineau J, Nanomaterials (Basel), 2021
Mechanical Control of Cell Migration by the Metastasis Suppressor Tetraspanin CD82/KAI1
Ordas L, Costa L, Lozano A, Chevillard C, Calovoulos A, Kantar D, Fernandez L, Chauvin L, Dosset P, Doucet C, Heron-Milhavet L, Odintsova E, Berditchevski F, Milhiet PE, Benistant C, Cells, 2021
Single-molecule FRET on its way to structural biology in live cells
Quast RB, Margeat E, Nat Methods, 2021
Cryo-electron microscopy structure of the antidiuretic hormone arginine-vasopressin V2 receptor signaling complex
Bous J, Orcel H, Floquet N, Leyrat C, Lai-Kee-Him J, Gaibelet G, Ancelin A, Saint-Paul J, Trapani S, Louet M, Sounier R, Demene H, Granier S, Bron P, Mouillac B, Sci Adv, 2021
Folding of phosphodiester-linked donor-acceptor oligomers into supramolecular nanotubes in water
Perez de Carvasal K, Aissaoui N, Vergoten G, Bellot G, Vasseur JJ, Smietana M, Morvan F, Chem Commun (Camb), 2021
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Lerner E, Barth A, Hendrix J, Ambrose B, Birkedal V, Blanchard SC, Borner R, Sung Chung H, Cordes T, Craggs TD, Deniz AA, Diao J, Fei J, Gonzalez RL, Gopich IV, Ha T, Hanke CA, Haran G, Hatzakis NS, Hohng S, Hong SC, Hugel T, Ingargiola A, Margeat E, Joo C, Kapanidis AN, Kim HD, Laurence T, Lee NK, Lee TH, Lemke EA, Michaelis J, Michalet X, Myong S, Nettels D, Peulen TO, Ploetz E, Razvag Y, Robb NC, Schuler B, Soleimaninejad H, Tang C, Vafabakhsh R, Lamb DC, Seidel CA, Weiss S, Elife, 2021
Resolving the activation mechanism of the D99N antiterminator LicT protein
Yang Y, Gracy J, Declerck N, Demene H, J Struct Biol, 2021
MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis
Kantar D, Mur EB, Mancini M, Slaninova V, Salah YB, Costa L, Forest E, Lassus P, Geminard C, Boissiere-Michot F, Orsetti B, Theillet C, Colinge J, Benistant C, Maraver A, Heron-Milhavet L, Djiane A, Sci Rep, 2021
Modular Imaging Scaffold for Single-Particle Electron Microscopy
Aissaoui N, Lai-Kee-Him J, Mills A, Declerck N, Morichaud Z, Brodolin K, Baconnais S, Le Cam E, Charbonnier JB, Sounier R, Granier S, Ropars V, Bron P, Bellot G, ACS Nano, 2021
Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization
Bada Juarez JF, Judge PJ, Adam S, Axford D, Vinals J, Birch J, Kwan TOC, Hoi KK, Yen HY, Vial A, Milhiet PE, Robinson CV, Schapiro I, Moraes I, Watts A, Nat Commun, 2021
Laminin gamma2 knockout mice rescued with the human protein exhibit enamel maturation defects
Wazen RM, Viegas-Costa LC, Fouillen A, Moffatt P, Adair-Kirk TL, Senior RM, Nanci A, Matrix Biol, 2021
2020
TAT-RasGAP317-326 kills cells by targeting inner-leaflet-enriched phospholipids
Serulla M, Ichim G, Stojceski F, Grasso G, Afonin S, Heulot M, Schober T, Roth R, Godefroy C, Milhiet PE, Das K, Garcia-Saez AJ, Danani A, Widmann C, Proc Natl Acad Sci U S A, 2020
Author Correction: STEEP mediates STING ER exit and activation of signaling
Zhang BC, Nandakumar R, Reinert LS, Huang J, Laustsen A, Gao ZL, Sun CL, Jensen SB, Troldborg A, Assil S, Berthelsen MF, Scavenius C, Zhang Y, Windross SJ, Olagnier D, Prabakaran T, Bodda C, Narita R, Cai Y, Zhang CG, Stenmark H, Doucet CM, Noda T, Guo Z, Goldbach-Mansky R, Hartmann R, Chen ZJ, Enghild JJ, Bak RO, Thomsen MK, Paludan SR, Nat Immunol, 2020
Author Correction: Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies
Fernandes TFD, Saavedra-Villanueva O, Margeat E, Milhiet PE, Costa L, Sci Rep, 2020
STEEP mediates STING ER exit and activation of signaling
Zhang BC, Nandakumar R, Reinert LS, Huang J, Laustsen A, Gao ZL, Sun CL, Jensen SB, Troldborg A, Assil S, Berthelsen MF, Scavenius C, Zhang Y, Windross SJ, Olagnier D, Prabakaran T, Bodda C, Narita R, Cai Y, Zhang CG, Stenmark H, Doucet CM, Noda T, Guo Z, Goldbach-Mansky R, Hartmann R, Chen ZJ, Enghild JJ, Bak RO, Thomsen MK, Paludan SR, Nat Immunol, 2020
Compression, Rupture, and Puncture of Model Membranes at the Molecular Scale
Saavedra V O, Fernandes TFD, Milhiet PE, Costa L, Langmuir, 2020
Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies
Fernandes TFD, Saavedra O, Margeat E, Milhiet PE, Costa L, Sci Rep, 2020
NMR chemical shift assignment of a constitutively active fragment of the antitermination protein LicT
Yang Y, Declerck N, Demene H, Biomol NMR Assign, 2020
Structural Insights into of the Allosteric Activation of the LicT Antiterminator by PTS-Mediated Phosphorylation
Yang Y, Padilla A, deGuillen K, Mammri L, Gracy J, Declerck N, Demene H, Structure, 2020
2019
NMR chemical shift backbone assignment of the viral protein P1 encoded by the African Rice Yellow Mottle Virus
Yang Y, Poignavent V, Gillet FX, Vignols F, Demene H, Biomol NMR Assign, 2019
Studying GPCR conformational dynamics by single molecule fluorescence
Quast RB, Margeat E, Mol Cell Endocrinol, 2019
Folding of the Ig-Like Domain of the Dengue Virus Envelope Protein Analyzed by High-Hydrostatic-Pressure NMR at a Residue-Level Resolution
Saotome T, Doret M, Kulkarni M, Yang YS, Barthe P, Kuroda Y, Roumestand C, Biomolecules, 2019
Nanoscale organization of tetraspanins during HIV-1 budding by correlative dSTORM/AFM
Dahmane S, Doucet C, Le Gall A, Chamontin C, Dosset P, Murcy F, Fernandez L, Salas D, Rubinstein E, Mougel M, Nollmann M, Milhiet PE, Nanoscale, 2019
Complex wireframe DNA nanostructures from simple building blocks
Wang W, Chen S, An B, Huang K, Bai T, Xu M, Bellot G, Ke Y, Xiang Y, Wei B, Nat Commun, 2019
Accurate Determination of Human CPR Conformational Equilibrium by smFRET using Dual Orthogonal Non-Canonical Amino Acid Labeling
Quast RB, Fatemi F, Kranendonk M, Margeat E, Truan G, Chembiochem, 2019
A fluorogenic assay to monitor Rho-dependent termination of transcription
Nadiras C, Delaleau M, Schwartz A, Margeat E, Boudvillain M, Biochemistry, 2019
Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy
Nasrallah H, Vial A, Pocholle N, Soulier J, Costa L, Godefroy C, Bourillot E, Lesniewska E, Milhiet PE, Methods Mol Biol, 2019
2018
Pathological interactions between mutant thyroid hormone receptors and corepressors and their modulation by a thyroid hormone analogue with therapeutic potential
Harrus D, Demene H, Vasquez EF, Boulahtouf A, Germain P, Figueira AC, Privalsky ML, Bourguet W, le Maire A, Thyroid, 2018
Chloride ions stabilize the glutamate-induced active state of the metabotropic glutamate receptor 3
Tora AS, Rovira X, Cao AM, Cabaye A, Olofsson L, Malhaire F, Scholler P, Baik H, Van Eeckhaut A, Smolders I, Rondard P, Margeat E, Acher F, Pin JP, Goudet C, Neuropharmacology, 2018
Pulling lipid tubes from supported bilayers unveils the underlying substrate contribution to the membrane mechanics
Gumi-Audenis B, Costa L, Ferrer-Tasies L, Ratera I, Ventosa N, Sanz F, Giannotti MI, Nanoscale, 2018
Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis
Guca E, Nagy GN, Hajdu F, Marton L, Izrael R, Hoh F, Yang Y, Vial H, Vertessy BG, Guichou JF, Cerdan R, Sci Rep, 2018
Oligomerization of a G protein-coupled receptor in neurons controlled by its structural dynamics
Mller TC, Hottin J, Clerte C, Zwier JM, Durroux T, Rondard P, Prezeau L, Royer CA, Pin JP, Margeat E, Kniazeff J, Sci Rep, 2018
Single-molecule analysis reveals the mechanism of transcription activation in
Vishwakarma RK, Cao AM, Morichaud Z, Perumal AS, Margeat E, Brodolin K, Sci Adv, 2018
Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation
Chara O, Borges A, Milhiet PE, Nollmann M, Cattoni DI, Sci Rep, 2018
2017
In-plane molecular organization of hydrated single lipid bilayers: DPPC:cholesterol
Gumi-Audenis B, Costa L, Redondo-Morata L, Milhiet PE, Sanz F, Felici R, Giannotti MI, Carla F, Nanoscale, 2017
Programmable self-assembly of three-dimensional nanostructures from 10,000 unique components
Ong LL, Hanikel N, Yaghi OK, Grun C, Strauss MT, Bron P, Lai-Kee-Him J, Schueder F, Wang B, Wang P, Kishi JY, Myhrvold C, Zhu A, Jungmann R, Bellot G, Ke Y, Yin P, Nature, 2017
Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation
Haining EJ, Matthews AL, Noy PJ, Romanska HM, Harris HJ, Pike J, Morowski M, Gavin RL, Yang J, Milhiet PE, Berditchevski F, Nieswandt B, Poulter NS, Watson SP, Tomlinson MG, Platelets, 2017
Angular reconstitution-based 3D reconstructions of nanomolecular structures from superresolution light-microscopy images
Salas D, Le Gall A, Fiche JB, Valeri A, Ke Y, Bron P, Bellot G, Nollmann M, Proc Natl Acad Sci USA, 2017
PEGylation rate influences peptide-based nanoparticles mediated siRNA delivery in vitro and in vivo
Aldrian G, Vaissiere A, Konate K, Seisel Q, Vives E, Fernandez F, Viguier V, Genevois C, Couillaud F, Demene H, Aggad D, Covinhes A, Barrere-Lemaire S, Deshayes S, Boisguerin P, J Control Release, 2017
Competitive folding of RNA structures at a termination / antitermination site
Ait-Bara S, Clerte C, Declerck N, Margeat E, RNA, 2017
Structural insights into adiponectin receptors suggest ceramidase activity
Vasiliauskaite-Brooks I, Sounier R, Rochaix P, Bellot G, Fortier M, Hoh F, De Colibus L, Bechara C, Saied EM, Arenz C, Leyrat C, Granier S, Nature, 2017
(1)H, (13)C and (15)N backbone chemical shift assignments of camelid single-domain antibodies against active state -opioid receptor
Sounier R, Yang Y, Hagelberger J, Granier S, Demene H, Biomol NMR Assign, 2017
2016
High-Resolution Mapping of a Repeat Protein Folding Free Energy Landscape
Fossat MJ, Dao TP, Jenkins K, Dellarole M, Yang Y, McCallum SA, Garcia AE, Barrick D, Roumestand C, Royer CA, Biophys J, 2016
The Human Mixed Lineage Leukemia 5 (MLL5), a Sequentially and Structurally Divergent SET Domain-Containing Protein with No Intrinsic Catalytic Activity
Mas-Y-Mas S, Barbon M, Teyssier C, Demene H, Carvalho JE, Bird LE, Lebedev A, Fattori J, Schubert M, Dumas C, Bourguet W, Le Maire A, PLoS One, 2016
Plasmonic Nanoantennas Enable Forbidden Forster Dipole-Dipole Energy Transfer and Enhance the FRET Efficiency
de Torres J, Mivelle M, Moparthi SB, Rigneault H, van Hulst NF, Garcia-Parajo MF, Margeat E, Wenger J, Nano Lett, 2016
A part toolbox to tune genetic expression in Bacillus subtilis
Guiziou S, Sauveplane V, Chang HJ, Clerte C, Declerck N, Jules M, Bonnet J, Nucleic Acids Res, 2016
Imaging HIV-1 RNA dimerization in cells by multicolor super-resolution and fluctuation microscopies
Ferrer M, Clerte C, Chamontin C, Basyuk E, Laine S, Hottin J, Bertrand E, Margeat E, Mougel M, Nucleic Acids Res, 2016
Epithelial Splicing Regulatory Protein 1 (ESRP1) is a new regulator of stomach smooth muscle development and plasticity
Sagnol S, Marchal S, Yang Y, Allemand F, de Stanta Barba P, Dev Biol, 2016
Optimisation of vectorisation property: a comparative study for a secondary amphipathic peptide
Konate K, Lindberg M, Vaissiere A, Jourdan C, Aldrian G, Margeat E, Deshayes S, Boisguerin P, Int J Pharm, 2016
Automatic detection of diffusion modes within biological membranes using back-propagation neural network
Dosset P, Rassam P, Fernandez L, Espenel C, Rubinstein E, Margeat E, Milhiet PE, BMC Bioinformatics, 2016
TOM1L1 drives membrane delivery of MT1-MMP to promote ERBB2-induced breast cancer cell invasion
Chevalier C, Collin G, Descamps S, Touaitahuata H, Simon V, Reymond N, Fernandez L, Milhiet PE, Georget V, Urbach S, Lasorsa L, Orsetti B, Boissiere-Michot F, Lopez-Crapez E, Theillet C, Roche S, Benistant C, Nat Commun, 2016
Structure and Nanomechanics of Model Membranes by Atomic Force Microscopy and Spectroscopy: Insights into the Role of Cholesterol and Sphingolipids
Gumi-Audenis B, Costa L, Carla F, Comin F, Sanz F, Giannotti MI, Membranes, 2016
2015
TspanC8 tetraspanins differentially regulate the cleavage of ADAM10 substrates, Notch activation and ADAM10 membrane compartmentalization
Jouannet S, Saint-Pol J, Fernandez L, Nguyen V, Charrin S, Boucheix C, Brou C, Milhiet PE, Rubinstein E, Cell Mol Life Sci, 2015
Membrane Curvature Sensing by Amphipathic Helices Is Modulated by the Surrounding Protein Backbone
Doucet CM, Esmery N, de Saint-Jean M, Antonny B, PLoS ONE, 2015
Propagation of conformational changes during mu-opioid receptor activation
Sounier R, Mas C, Steyaert J, Laeremans T, Manglik A, Huang W, Kobilka BK, Demene H, Granier S, Nature, 2015
Direct observation of the translocation mechanism of transcription termination factor Rho
Gocheva V, Le Gall A, Boudvillain M, Margeat E, Nollmann M, Nucleic Acids Res, 2015
HIV-1 nucleocapsid and ESCRT-component Tsg101 interplay prevents HIV from turning into a DNA-containing virus
Chamontin C, Rassam P, Ferrer M, Racine PJ, Neyret A, Laine S, Milhiet PE, Mougel M, Nucleic Acids Res, 2015
Single-Molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein
Ait-Bara S, Clerte C, Margeat E, Methods Mol Biol, 2015
2014
Chromatin Insulator Factors Involved in Long-Range DNA Interactions and Their Role in the Folding of the Drosophila Genome
Vogelmann J, Le Gall A, Dejardin S, Allemand F, Gamot A, Labesse G, Cuvier O, Negre N, Cohen-Gonsaud M, Margeat E, Nollmann M, PLoS Genet, 2014
Prioritizing targets for structural biology through the lens of proteomics: The archaeal protein TGAM_1934 from Thermococcus gammatolerans
Yang YS, Fernandez B, Lagorce A, Aloin V, deGuillen KM, Boyer JB, Dedieu A, Confalonieri F, Armengaud J, Roumestand C, Proteomics, 2014
(1)H, (15)N and (13)C Backbone resonance assignments of murine met-neuroglobin, free and in complex with cyanide
Yang Y, Allemand F, Guca E, Vallone B, Delbecq S, Roumestand C, Biomol NMR Assign, 2014
The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved Nucleotide Analog Interference Probing (trNAIP)
Soares E, Schwartz A, Nollmann M, Margeat E, Boudvillain M, Nucleic Acids Res, 2014
Homodimerization of RBPMS2 through a new RRM-interaction motif is necessary to control smooth muscle plasticity
Sagnol S, Yang Y, Bessin Y, Allemand F, Hapkova I, Notarnicola C, Guichou JF, Faure S, Labesse G, de Santa Barbara P, Nucleic Acids Res, 2014
Fine tuning of sub-millisecond conformational dynamics controls metabotropic glutamate receptors agonist efficacy
Olofsson L, Felekyan S, Doumazane E, Scholler P, Fabre L, Zwier JM, Rondard P, Seidel CA, Pin JP, Margeat E, Nat Commun, 2014
Structural and Biochemical Characterization of the Cop9 Signalosome CSN5/CSN6 Heterodimer
Birol M, Enchev RI, Padilla A, Stengel F, Aebersold R, Betzi S, Yang Y, Hoh F, Peter M, Dumas C, Echalier A, PLoS ONE, 2014
Viruses and tetraspanins: lessons from single molecule approaches
Dahmane S, Rubinstein E, Milhiet PE, Viruses, 2014
Mimicking influenza virus fusion using supported lipid bilayers
Godefroy C, Dahmane S, Dosset P, Adam O, Nicolai MC, Ronzon F, Milhiet PE, Langmuir, 2014
Characterization of the structural modifications accompanying the loss of HBsAg particle immunogenicity
Greiner VJ, Manin C, Larquet E, Ikhelef N, Greco F, Naville S, Milhiet PE, Ronzon F, Klymchenko A, Mely Y, Vaccine, 2014
Milk sphingomyelin domains in biomimetic membranes and the role of cholesterol: morphology and nanomechanical properties investigated using AFM and force spectroscopy
Guyomarc'h F, Zou S, Chen M, Milhiet PE, Godefroy C, Vie V, Lopez C, Langmuir, 2014
Stoichiometry of scaffold complexes in living neurons - DLC2 as a dimerization engine for GKAP
Moutin E, Compan V, Raynaud F, Clerte C, Bouquier N, Labesse G, Ferguson ML, Fagni L, Royer CA, Perroy J, J Cell Sci, 2014
2013
EWI-2wint promotes CD81 clustering that abrogates Hepatitis C Virus entry
Potel J, Rassam P, Montpellier C, Kaestner L, Werkmeister E, Tews BA, Couturier C, Popescu CI, Baumert TF, Rubinstein E, Dubuisson J, Milhiet PE, Cocquerel L, Cell Microbiol, 2013
Pulsed interleaved excitation fluorescence spectroscopy with a supercontinuum source
Olofsson L, Margeat E, Opt Express, 2013
Imaging of transmembrane proteins directly incorporated within supported lipid bilayers using atomic force microscopy
Levy D, Milhiet PE, Methods Mol Biol, 2013
Hepatoma polarization limits CD81 and hepatitis C virus dynamics
Harris HJ, Clerte C, Farquhar MJ, Goodall M, Hu K, Rassam P, Dosset P, Wilson GK, Balfe P, Ijzendoorn SC, Milhiet PE, McKeating JA, Cell Microbiol, 2013
Interaction of antibiotics with lipid vesicles on thin film porous silicon using reflectance interferometric Fourier transform spectroscopy
Guinan T, Godefroy C, Lautredou N, Pace S, Milhiet PE, Voelcker N, Cunin F, Langmuir, 2013
Recruitment, assembly, and molecular architecture of the SpoIIIE DNA pump revealed by superresolution microscopy
Fiche JB, Cattoni DI, Diekmann N, Langerak JM, Clerte C, Royer CA, Margeat E, Doan T, Nollmann M, PLoS Biol, 2013
Equivalence between Euler angle conventions for the description of tensorial interactions in liquid NMR: application to different software programs
Dosset P, Barthe P, Cohen-Gonsaud M, Roumestand C, Demene H, J Biomol NMR, 2013
Negative regulation of estrogen signaling by ERbeta and RIP140 in ovarian cancer cells
Docquier A, Garcia A, Savatier J, Boulahtouf A, Bonnet S, Bellet V, Busson M, Margeat E, Jalaguier S, Royer C, Balaguer P, Cavailles V, Mol Endocrinol, 2013
Competitive folding of anti-terminator/terminator hairpins monitored by single molecule FRET
Clerte C, Declerck N, Margeat E, Nucleic Acids Res, 2013
Structure and DNA-binding properties of the Bacillus subtilis SpoIIIE DNA translocase revealed by single-molecule and electron microscopies
Cattoni DI, Thakur S, Godefroy C, Le Gall A, Lai-Kee-Him J, Milhiet PE, Bron P, Nollmann M, Nucleic Acids Res, 2013
SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
Cattoni DI, Chara O, Godefroy C, Margeat E, Trigueros S, Milhiet PE, Nollmann M, EMBO Rep, 2013
2012
Low concentration of a Gd-chelate increases the signal-to-noise ratio in fast pulsing BEST experiments.
Sibille N, Bellot G, Wang J, Demene H, J. Magn. Reson., 2012
Structural and functional characterization of Bc28.1, major erythrocyte-binding protein from Babesia canis merozoite surface
Yang YS, Murciano B, Moubri K, Cibrelus P, Schetters T, Gorenflot A, Delbecq S, Roumestand C, J Biol Chem, 2012
The C-terminal domain of the virulence factor MgtC is a divergent ACT domain.
Yang Y, Labesse G, Carrere-Kremer S, Esteves K, Kremer L, Cohen-Gonsaud M, Blanc-Potard AB, J. Bacteriol., 2012
Structural characterization by nuclear magnetic resonance of the impact of phosphorylation in the proline-rich region of the disordered Tau protein
Sibille N, Huvent I, Fauquant C, Verdegem D, Amniai L, Leroy A, Wieruszeski JM, Lippens G, Landrieu I, Proteins, 2012
Analysis of helicase-RNA interactions using nucleotide analog interference mapping
Schwartz A, Rabhi M, Margeat E, Boudvillain M, Methods Enzymol, 2012
Remodeling of the folding free energy landscape of staphylococcal nuclease by cavity-creating mutations.
Roche J, Dellarole M, Caro JA, Guca E, Norberto DR, Yang Y, Garcia AE, Roumestand C, Garcia-Moreno B, Royer CA, Biochemistry, 2012
Atomic force microscopy: a versatile tool to probe the physical and chemical properties of supported membranes at the nanoscale.
Picas L, Milhiet PE, Hernandez-Borrell J, Chem. Phys. Lipids, 2012
Characterization of phospholipid bilayer formation on a thin film of porous SiO2 by reflective interferometric Fourier transform spectroscopy (RIFTS).
Pace S, Seantier B, Belamie E, Lautredou N, Sailor MJ, Milhiet PE, Cunin F, Langmuir, 2012
Self-assembling peptide-based nanoparticles for siRNA delivery in primary cell lines.
Deshayes S, Konate K, Rydstrom A, Crombez L, Godefroy C, Milhiet PE, Thomas A, Brasseur R, Aldrian G, Heitz F, Munoz-Morris MA, Devoisselle JM, Divita G, Small, 2012
2011
Expression, purification and NMR characterization of the cyclic recombinant form of the third intracellular loop of the vasopressin type 2 receptor
Bellot G, Pascal R, Mendre C, Urbach S, Mouillac B, Demene H, Protein Expr Purif, 2011
Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase
Roumestand C, Leiba J, Galophe N, Margeat E, Padilla A, Bessin Y, Barthe P, Molle V, Cohen-Gonsaud M, Structure, 2011
Structure of the Mycobacterium tuberculosis OmpATb protein: a model of an oligomeric channel in the mycobacterial cell wall
Yang Y, Auguin D, Delbecq S, Dumas E, Molle G, Molle V, Roumestand C, Saint N, Proteins, 2011
Transfer on hydrophobic substrates and AFM imaging of membrane proteins reconstituted in planar lipid bilayers
Seantier B, Dezi M, Gubellini F, Berquand A, Godefroy C, Dosset P, Levy D, Milhiet PE, J Mol Recognit, 2011
Mutagenesis-based evidence for an asymmetric configuration of the ring-shaped transcription termination factor Rho
Rabhi M, Gocheva V, Jacquinot F, Lee A, Margeat E, Boudvillain M, J Mol Biol, 2011
Nanoscale topography of hepatitis B antigen particles by atomic force microscopy
Milhiet PE, Dosset P, Godefroy C, Le Grimellec C, Guigner JM, Larquet E, Ronzon F, Manin C, Biochimie, 2011
2010
Preferential insertion of lactose permease in phospholipid domains: AFM observations
Picas L, Carretero-Genevrier A, Montero MT, Vazquez-Ibar JL, Seantier B, Milhiet PE, Hernandez-Borrell J, Biochim Biophys Acta, 2010
A Grafting of monoglyceride molecules for the design of hydrophilic and stable porous silicon surfaces
Pace S, Gonzalez P, Devoisselle J, Milhiet P, Brunel D, Cunin F, N.J. Chem, 2010
Deciphering the structure, growth and assembly of amyloid-like fibrils using high-speed atomic force microscopy
Milhiet PE, Yamamoto D, Berthoumieu O, Dosset P, Le Grimellec C, Verdier JM, Marchal S, Ando T, PLoS One, 2010
Transfert d'energie de Forster sur molecules uniques : applications en biologie
Margeat E, L'Actualite Chimique, 2010
HIV-1 assembly differentially alters dynamics and partitioning of tetraspanins and raft components
Krementsov DN, Rassam P, Margeat E, Roy NH, Schneider-Schaulies J, Milhiet PE, Thali M, Traffic, 2010
Surface topography of membrane domains
Giocondi MC, Yamamoto D, Lesniewska E, Milhiet PE, Ando T, Le Grimellec C, Biochim Biophys Acta, 2010
Organization of influenza A virus envelope at neutral and low pH
Giocondi MC, Ronzon F, Nicolai MC, Dosset P, Milhiet PE, Chevalier M, Le Grimellec C, J Gen Virol, 2010
The gelsolin:calponin complex nucleates actin filaments with distinct morphologies
Ferjani I, Fattoum A, Bettache N, Seantier B, Milhiet PE, Manai M, Benyamin Y, Roustan C, Maciver SK, Biochem Biophys Res Commun, 2010
Keeping up to speed with the transcription termination factor Rho motor
Boudvillain M, Nollmann M, Margeat E, Transcription, 2010
2009
Structure of the third intracellular loop of the vasopressin V2 receptor and conformational changes upon binding to gC1qR
Bellot G, Granier S, Bourguet W, Seyer R, Rahmeh R, Mouillac B, Pascal R, Mendre C, Demene H, J Mol Biol, 2009
Lateral organization of membrane proteins: tetraspanins spin their web
Charrin S, le Naour F, Silvie O, Milhiet PE, Boucheix C, Rubinstein E, Biochem J, 2009
Calcium-induced formation of subdomains in phosphatidylethanolamine-phosphatidylglycerol bilayers: a combined DSC, 31P NMR, and AFM study
Picas L, Montero MT, Morros A, Cabanas ME, Seantier B, Milhiet PE, Hernandez-Borrell J, J Phys Chem B, 2009
Dynamic Partitioning of a Glycosyl-Phosphatidylinositol-Anchored Protein in Glycosphingolipid-Rich Microdomains Imaged by Single-Quantum Dot Tracking
Pinaud F, Michalet X, Iyer G, Margeat E, Moore HP, Weiss S, Traffic, 2009
A stepwise 2'-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase
Schwartz A, Rabhi M, Jacquinot F, Margeat E, Rahmouni AR, Boudvillain M, Nat Struct Mol Biol, 2009
NMR structure of rALF-Pm3, an anti-lipopolysaccharide factor from shrimp: model of the possible lipid A-binding site
Yang Y, Boze H, Chemardin P, Padilla A, Moulin G, Tassanakajon A, Pugniere M, Roquet F, Destoumieux-Garzon D, Gueguen Y, Bachere E, Aumelas A, Biopolymers, 2009
Structural characterization of the mammalian deoxynucleotide N-hydrolase Rcl and its stabilizing interactions with two inhibitors
Yang Y, Padilla A, Zhang C, Labesse G, Kaminski PA, J Mol Biol, 2009
2008
Inhibition of the mitogenic, angiogenic and tumorigenic activities of pleiotrophin by a synthetic peptide corresponding to its C-thrombospondin repeat-I domain
Hamma-Kourbali Y, Bernard-Pierrot I, Heroult M, Dalle S, Caruelle D, Milhiet PE, Fernig DG, Delbe J, Courty J, J Cell Physiol, 2008
Conformational change upon ligand binding and dynamics of the PDZ domain from leukemia-associated Rho guanine nucleotide exchange factor
Liu J, Zhang J, Yang Y, Huang H, Shen W, Hu Q, Wang X, Wu J, Shi Y, Protein Sci, 2008
NMR structure of mussel mytilin, and antiviral-antibacterial activities of derived synthetic peptides
Roch P, Yang YS, Toubiana M, Aumelas A, Dev Comp Immunol, 2008
Probing supporting model and native membranes using afm
Seantier B, Giocondi MC, Le Grimellec C, Milhiet PE, Current Opinion in Colloid and Interface Science, 2008
Investigating transcriptional regulation by fluorescence spectroscopy, from traditional methods to state-of-the-art single-molecule approaches
Zorrilla S, Lillo MP, Chaix D, Margeat E, Royer CA, Declerck N, Ann N Y Acad Sci, 2008
Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM
Giocondi MC, Seantier B, Dosset P, Milhiet PE, Le Grimellec C, Pflugers Arch, 2008
Single-molecule analysis of CD9 dynamics and partitioning reveals multiple modes of interaction in the tetraspanin web
Espenel C, Margeat E, Dosset P, Arduise C, Le Grimellec C, Royer CA, Boucheix C, Rubinstein E, Milhiet PE, J Cell Biol, 2008
Temperature-dependent imaging of living cells by AFM
Espenel C, Giocondi MC, Seantier B, Dosset P, Milhiet PE, Le Grimellec C, Ultramicroscopy, 2008
Structural mechanism of signal transduction between the RNA-binding domain and the phosphotransferase system regulation domain of the LicT antiterminator
Demene H, Ducat T, DeGuillen K, Birck C, Aymerich S, Kochoyan M, Declerck N, J Biol Chem, 2008
The solution structure of the adhesion protein Bd37 from Babesia divergens reveals structural homology with eukaryotic proteins involved in membrane trafficking
Delbecq S, Auguin D, Yang YS, Lohr F, Arold ST, Schetters T, Precigout E, Gorenflot A, Roumestand C, J Mol Biol, 2008
Quantitative analysis of the binding of ezrin to large unilamellar vesicles containing phosphatidylinositol 4,5 bisphosphate
Blin G, Margeat E, Carvalho K, Royer CA, Roy C, Picart C, Biophys J, 2008
The multifunctional protein GC1q-R interacts specifically with the i3 loop arginine cluster of the vasopressin V2 receptor
Granier S, Jean-Alphonse F, Bacqueville D, Monteil A, Pascal R, Poncet J, Guillon G, Boudier L, Arcemisbehere L, Mouillac B, Bellot G, Demene H, Mendre C, Regul Pept, 2008
2007
Nanoforce et Imagerie AFM
Le Grimellec C, Milhiet PE, Perez E, Pincet F, Aime JP, Emiliani V, Thoumine O, Lionnet T, Croquette V, Allemand JF, Bensimon D, Les Nanosciences: Nanobiotechnologies et nanobiologie, 2007
Alternating laser excitation of single molecules
Kapanidis AN, Heilemann M, Margeat E, Kong X, Nir E, Weiss S, Single Molecule Techniques : A Laboratory Manual , 2007
Inducer-modulated cooperative binding of the tetrameric CggR repressor to operator DNA
Zorrilla S, Doan T, Alfonso C, Margeat E, Ortega A, Rivas G, Aymerich S, Royer CA, Declerck N, Biophys J, 2007
Fructose-1,6-bisphosphate acts both as an inducer and as a structural cofactor of the central glycolytic genes repressor (CggR)
Zorrilla S, Chaix D, Ortega A, Alfonso C, Doan T, Margeat E, Rivas G, Aymerich S, Declerck N, Royer CA, Biochemistry, 2007
Domain-swapped dimerization of the second PDZ domain of ZO2 may provide a structural basis for the polymerization of claudins
Wu J, Yang Y, Zhang J, Ji P, Du W, Jiang P, Xie D, Huang H, Wu M, Zhang G, Wu J, Shi Y, J Biol Chem, 2007
Transcription termination factor Rho can displace streptavidin from biotinylated RNA
Schwartz A, Margeat E, Rahmouni AR, Boudvillain M, J Biol Chem, 2007
The Characterization of Bio-molecular Interactions Using Fluorescence Fluctuation Techniques
Margeat E, Boukari, H., Royer, C.A., Protein Reviews, 2007
Remodeling of ordered membrane domains by GPI-anchored intestinal alkaline phosphatase
Giocondi MC, Besson F, Dosset P, Milhiet PE, Le Grimellec C, Langmuir, 2007
Temperature-dependent localization of GPI-anchored intestinal alkaline phosphatase in model rafts
Giocondi MC, Besson F, Dosset P, Milhiet PE, Le Grimellec C, J Mol Recognit, 2007
Continuous planar phospholipid bilayer supported on porous silicon thin film reflector
Cunin F, Milhiet PE, Anglin E, Sailor MJ, Espenel C, Le Grimellec C, Brunel D, Devoisselle JM, Ultramicroscopy, 2007
Influence of calcium on direct incorporation of membrane proteins into in-plane lipid bilayer
Berquand A, Levy D, Gubellini F, Le Grimellec C, Milhiet PE, Ultramicroscopy, 2007
Glycosylation status of the membrane protein CD9P-1
Andre M, Morelle W, Planchon S, Milhiet PE, Rubinstein E, Mollicone R, Chamot-Rooke J, Le Naour F, Proteomics, 2007
2006
The Characterization of Bio-molecular Interactions Using Fluorescence Fluctuation Techniques
Margeat E, Boukari H, Royer CA, Protein Interactions, 2006
High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer
Milhiet PE, Gubellini F, Berquand A, Dosset P, Rigaud JL, Le Grimellec C, Levy D, Biophys J, 2006
Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes
Margeat E, Kapanidis AN, Tinnefeld P, Wang Y, Mukhopadhyay J, Ebright RH, Weiss S, Biophys J, 2006
Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism
Kapanidis AN, Margeat E, Ho SO, Kortkhonjia E, Weiss S, Ebright RH, Science, 2006
Rapid determination of protein solubility and stability conditions for NMR studies using incomplete factorial design
Ducat T, Declerck N, Gostan T, Kochoyan M, Demene H, J Biomol NMR, 2006
Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs
Granier S, Jean-Alphonse F, Demene H, Guillon G, Pascal R, Mendre C, Bioorg Med Chem Lett, 2006
PenBase, the shrimp antimicrobial peptide penaeidin database: sequence-based classification and recommended nomenclature
Gueguen Y, Garnier J, Robert L, Lefranc MP, Mougenot I, de Lorgeril J, Janech M, Gross PS, Warr GW, Cuthbertson B, Barracco MA, Bulet P, Aumelas A, Yang Y, Bo D, Xiang J, Tassanakajon A, Piquemal D, Bachere E, Dev Comp Immunol, 2006
2005
1H, 15N and 13C Backbone resonance assignments of the 37 kDa surface antigen protein Bd37 from Babesia divergens
Yang YS, Delbecq S, Strub MP, Lohr F, Schetters T, Gorenflot A, Precigout E, Roumestand C, J Biomol NMR, 2005
Coexistence of a two-states organization for a cell-penetrating peptide in lipid bilayer
Plenat T, Boichot S, Dosset P, Milhiet PE, Le Grimellec C, Biophys J, 2005
Alternating-laser excitation of single molecules
Kapanidis AN, Laurence TA, Lee NK, Margeat E, Kong X, Weiss S, Acc Chem Res, 2005
Solution structure of synthetic penaeidin-4 with structural and functional comparisons with penaeidin-3
Cuthbertson BJ, Yang Y, Bachere E, Bullesbach EE, Gross PS, Aumelas A, J Biol Chem, 2005
Carbocyanine dyes as efficient reversible single-molecule optical switch
Heilemann M, Margeat E, Kasper R, Sauer M, Tinnefeld P, J Am Chem Soc, 2005
Retention of transcription initiation factor sigma70 in transcription elongation: single-molecule analysis
Kapanidis AN, Margeat E, Laurence TA, Doose S, Ho SO, Mukhopadhyay J, Kortkhonjia E, Mekler V, Ebright RH, Weiss S, Mol Cell, 2005
2004
Use of cyclodextrin for AFM monitoring of model raft formation
Giocondi MC, Milhiet PE, Dosset P, Le Grimellec C, Biophys J, 2004
Heparin affin regulatory peptide in milk: its involvement in mammary gland homeostasis
Bernard-Pierrot I, Delbe J, Heroult M, Rosty C, Soulie P, Barritault D, Milhiet PE, Courty J, Biochem Biophys Res Commun, 2004
Interaction of primary amphipathic cell-penetrating peptides with phospholipid-supported monolayers
Plenat T, Deshayes S, Boichot S, Milhiet PE, Cole RB, Heitz F, Le Grimellec C, Langmuir, 2004
Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules
Kapanidis AN, Lee NK, Laurence TA, Doose S, Margeat E, Weiss S, Proc Natl Acad Sci U S A, 2004
Calcitonin-derived carrier peptide plays a major role in the membrane localization of a peptide-cargo complex
Boichot S, Krauss U, Plenat T, Rennert R, Milhiet PE, Beck-Sickinger A, Le Grimellec C, FEBS Lett, 2004
A cyclic peptide mimicking the third intracellular loop of the V2 vasopressin receptor inhibits signaling through its interaction with receptor dimer and G protein
Granier S, Terrillon S, Pascal R, Demene H, Bouvier M, Guillon G, Mendre C, J Biol Chem, 2004
2003
The H-NS dimerization domain defines a new fold contributing to DNA recognition
Bloch V, Yang Y, Margeat E, Chavanieu A, Auge MT, Robert B, Arold S, Rimsky S, Kochoyan M, Nat Struct Biol, 2003
NMR structure of the cathelin-like domain of the protegrin-3 precursor
Yang Y, Sanchez JF, Strub MP, Brutscher B, Aumelas A, Biochemistry, 2003
Solution structure of the recombinant penaeidin-3, a shrimp antimicrobial peptide
Yang Y, Poncet J, Garnier J, Zatylny C, Bachere E, Aumelas A, J Biol Chem, 2003
RAR Antagonists Diminish the Level of DNA Binding by the RAR/RXR Heterodimer
Poujol N, Margeat E, Baud S, Royer CA, Biochemistry, 2003
Ligands differentially modulate the protein interactions of the human estrogen receptors alpha and beta
Margeat E, Bourdoncle A, Margueron R, Poujol N, Cavailles V, Royer C, J Mol Biol, 2003
Crystal structure of the N-terminal dimerisation domain of VicH, the H-NS-like protein of Vibrio cholerae
Cerdan R, Bloch V, Yang Y, Bertin P, Dumas C, Rimsky S, Kochoyan M, Arold ST, J Mol Biol, 2003
Active peptidic mimics of the second intracellular loop of the V(1A) vasopressin receptor are structurally related to the second intracellular rhodopsin loop: a combined 1H NMR and biochemical study
Demene H, Granier S, Muller D, Guillon G, Dufour MN, Delsuc MA, Hibert M, Pascal R, Mendre C, Biochemistry, 2003
AFM imaging of lipid domains in model membranes
Milhiet PE, Giocondi MC, Le Grimellec C, ScientificWorldJournal, 2003
From cell imaging to molecular manipulation: biological applications of atomic force microscopy
Giocondi MC, Milhiet PE, Lesniewska E, Le Grimellec C, M S Medecine Sciences, 2003
2002
Equilibrium binding assays reveal the elevated stoichiometry and salt dependence of the interaction between full-length human sex-determining region on the Y chromosome (SRY) and DNA
Baud S, Margeat E, Lumbroso S, Paris F, Sultan C, Royer C, Poujol N, J Biol Chem, 2002
Solution structure of the LicT-RNA antitermination complex: CAT clamping RAT
Yang Y, Declerck N, Manival X, Aymerich S, Kochoyan M, Embo J, 2002
Overexpression and structural study of the cathelicidin motif of the protegrin-3 precursor
Sanchez JF, Wojcik F, Yang YS, Strub MP, Strub JM, Van Dorsselaer A, Martin M, Lehrer R, Ganz T, Chavanieu A, Calas B, Aumelas A, Biochemistry, 2002
Cholesterol is not crucial for the existence of microdomains in kidney brush-border membrane models
Milhiet PE, Giocondi MC, Le Grimellec C, J Biol Chem, 2002
Spontaneous insertion and partitioning of alkaline phosphatase into model lipid rafts
Milhiet PE, Giocondi MC, Baghdadi O, Ronzon F, Roux B, Le Grimellec C, EMBO Rep, 2002
AFM detection of GPI protein insertion into DOPC/DPPC model membranes
Milhiet PE, Giocondi MC, Baghdadi O, Ronzon F, Le Grimellec C, Roux B, Single Mol., 2002
Atomic force microscope imaging of cells and membranes
Lesniewska E, Milhiet PE, Giocondi MC, Le Grimellec C, Methods Cell Biol, 2002
Helix motion in protein C12A-p8(MTCP1): comparison of molecular dynamics simulations and multifield NMR relaxation data
Barthe P, Roumestand C, Demene H, Chiche L, J. Comput. Chem., 2002
Structure refinement of flexible proteins using dipolar couplings: application to the protein p8MTCP1
Demene H, Ducat T, Barthe P, Delsuc MA, Roumestand C, J. Biomol. NMR., 2002
MabA (FabG1), a Mycobacterium tuberculosis protein involved in the long-chain fatty acid elongation system FAS-II
Marrakchi H, Ducasse S, Labesse G, Montrozier H, Margeat E, Emorine L, Charpentier X, Daffe M, Quemard A, Microbiology, 2002
1H, 15N and 13C backbone resonance assignments of the 40 kDa LicT-CAT-PRD1 protein
Ducat T, Declerck N, Kochoyan M, Demene H, J Biomol NMR, 2002
RNA recognition by transcriptional antiterminators of the BglG/SacY family: mapping of SacY RNA binding site
Declerck N, Minh NL, Yang Y, Bloch V, Kochoyan M, Aymerich S, J Mol Biol, 2002
2001
Translocation of the pAntp peptide and its amphipathic analogue AP-2AL
Drin G, Demene H, Temsamani J, Brasseur R, Biochemistry, 2001
AFM characterization of model rafts in supported bilayers
Milhiet PE, Vie V, Giocondi MC, Le Grimellec C, Single Mol., 2001
Domain formation in models of the renal brush border membrane outer leaflet
Milhiet PE, Domec C, Giocondi MC, Van Mau N, Heitz F, Le Grimellec C, Biophys J, 2001
Phase topology and growth of single domains in lipid bilayers
Giocondi MC, Vie V, Lesniewska E, Milhiet PE, Zinke-Allmang M, Le Grimellec C, Langmuir, 2001
Temperature dependence of the topology of supported dimirystoyl- distearoyl phosphatidylcholine bilayers
Giocondi MC, Pacheco L, Milhiet PE, Le Grimellec C, Ultramicroscopy, 2001
The human estrogen receptor alpha dimer binds a single SRC-1 coactivator molecule with an affinity dictated by agonist structure
Margeat E, Poujol N, Boulahtouf A, Chen Y, Muller JD, Gratton E, Cavailles V, Royer CA, J Mol Biol, 2001
2000
Quantitative characterization of the interaction between purified human estrogen receptor alpha and DNA using fluorescence anisotropy
Boyer M, Poujol N, Margeat E, Royer CA, Nucleic Acids Res, 2000
Solution structure and activity of the synthetic four-disulfide bond Mediterranean mussel defensin (MGD-1)
Yang YS, Mitta G, Chavanieu A, Calas B, Sanchez JF, Roch P, Aumelas A, Biochemistry, 2000
Backbone dynamics and solution structure refinement of the 15N-labeled human oncogenic protein p13MTCP1: comparison with X-ray data
Guignard L, Padilla A, Mispelter J, Yang YS, Stern MH, Lhoste JM, Roumestand C, J Biomol NMR, 2000
1999
Rational engineering of a miniprotein that reproduces the core of the CD4 site interacting with HIV-1 envelope glycoprotein
Vita C, Drakopoulou E, Vizzavona J, Rochette S, Martin L, Menez A, Roumestand C, Yang YS, Ylisastigui L, Benjouad A, Gluckman JC, Proc Natl Acad Sci U S A, 1999
Effect of ligand and DNA binding on the interaction between human transcription intermediary factor 1alpha and estrogen receptors
Thenot S, Bonnet S, Boulahtouf A, Margeat E, Royer CA, Borgna JL, Cavailles V, Mol Endocrinol, 1999
1998
Solution structure of the recombinant human oncoprotein p13MTCP1
Yang YS, Guignard L, Padilla A, Hoh F, Strub MP, Stern MH, Lhoste JM, Roumestand C, J Biomol NMR, 1998
The preliminary solution structure of human p8MTCP1, a protein encoded by the putative MTCP1 oncogene
Barthe P, Guignard L, Yang YS, Chiche L, Strub MP, Hoh F, Stern MH, Roumestand C, J. Chim. Phys., 1998
Visualization of trp repressor and its complexes with DNA by atomic force microscopy
Margeat E, Le Grimellec C, Royer CA, Biophys J, 1998
Crystal structure of p14TCL1, an oncogene product involved in T-cell prolymphocytic leukemia, reveals a novel beta-barrel topology
Hoh F, Yang YS, Guignard L, Padilla A, Stern MH, Lhoste JM, van Tilbeurgh H, Structure, 1998
1997
Solution structure of human p8MTCP1, a cysteine-rich protein encoded by the MTCP1 oncogene, reveals a new alpha-helical assembly motif
Barthe P, Yang YS, Chiche L, Hoh F, Strub MP, Guignard L, Soulier J, Stern MH, van Tilbeurgh H, Lhoste JM, Roumestand C, J. Mol. Biol., 1997
From genetic to structural characterization of a new class of RNA-binding domain within the SacY/BglG family of antiterminator proteins
Manival X, Yang Y, Strub MP, Kochoyan M, Steinmetz M, Aymerich S, Embo J, 1997
1996
| Director | Emmanuel Margeat |
| Deputy directors | Vanessa Delfosse Martin Cohen-Gonsaud |
| Admin.Resp. | Florence Lepage |
| (33) 04 67 41 79 01 |
| Manager.Assist. | Marion Boutin |
| (33) 04 67 41 79 04 |