Plateforme Intégrée de Biophysique et de Biologie Structurale


HP NMR and screening


Folding pathway of proteins at the residue level. Ligand screening at low throughput.
MW. Below 15 kDa for HP NMR

Series of HSQC spectra are recorded at increasing hydrostatic pressure.
Thermodynamic analysis allows characterizing the unfolding/folding pathway.
Output : Series of thermodynamic parameters and intermediate structures.

Top 3 panels : HSQC spectra, Top-right : Intensity vs. Pressure, Bottom Panels: Structure Contact Maps upon unfolding


700 MHz NMR spectrometer


600 MHz NMR spectrometer


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