Vitrobot (ThermoFischer)
| Equipement |
Vitrobot (ThermoFischer) |
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Frozen hydrated sample preparation |
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| Room | Z3-9@CBS29 | |
Pumping Station for cryo holders
| Equipement |
Pumping Station (Gatan) for cryo holders |
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| Measure |
Structural analysis of frozen hydrated single particles, Cryo electron tomography, micro electron diffraction |
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For cryo holders |
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| Room | Z3-9@CBS29 | |
Jeol 1400
| Equipement |
Cryo Electron Microscope |
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| Measure |
Sample analysis in negative stain, electron tomography or frozen hydrated sample |
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EM to analyse singles particles samples in negative stain,1400 frozen hydrated sample, or cells by electron tomography |
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JEOL 1400 Flash transmission electron microscope |
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| Specifications |
It is a LaB6 microscope working at 120kV, equipped with a GATAN One View camera to record images. This microscope is open to every user after training, to screen and to check their sample quality, then, have a 2D classes after acquisition on negative stained samples. This microscope is also equipped with cryo pole piece to allow frozen hydrated samples observation. The implemented Shot Meister software allows low dose acquisition, suitable for biological samples that are highly sensitive to electron damages. For cryo electron tomography, Serial EM software is used to collect tilted series from -60° to +60°. https://www.jeolusa.com/PRODUCTS/Transmission-Electron-Microscopes-TEM |
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| Room | Z3-4@CBS29 | |
Jeol 2200FS Cryo Electron Microscope
| Equipement |
Cryo Electron Microscope |
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| Measure |
Structural analysis of frozen hydrated single particles, Cryo electron tomography, micro electron diffraction |
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CryoEM to analyse frozen hydrated single particles samples, or by electron tomography or electron micro diffraction |
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JEOL 2200FS transmission electron microscope |
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| Specifications |
It is a FEG microscope working at 200kV, equipped with a GATAN direct detector K3 camera (installed in 2021) to record images. This microscope have a cryo pole piece to allow frozen hydrated samples observation, it is also have a in-column energy filter (Omega filter). The implemented iMDS software allows low dose acquisition, suitable for biological samples that are highly sensitive to electron damages. For cryo electron tomography, Serial EM software is used to collect tilted series from -60° to +60°. For electron micro diffraction, the ASI Cheetah camera (1M Pixels) will be installed in the near future. https://www.jeolusa.com/PRODUCTS/Transmission-Electron-Microscopes-TEM |
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| Room | Z3-9@CBS29 | |