Pierre-Emmanuel Milhiet
Structure et Dynamique d’Assemblages Membranaires
04 67 41 79 17
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Liste des publications
2024
2023
Phase separation and molecular ordering of the prion-like domain of the Arabidopsis thermosensory protein EARLY FLOWERING 3
Hutin S, Kumita JR, Strotmann VI, Dolata A, Ling WL, Louafi N, Popov A, Milhiet PE, Blackledge M, Nanao MH, Wigge PA, Stahl Y, Costa L, Tully MD, Zubieta C, Proc Natl Acad Sci U S A, 2023
A Tripartite Complex HIV-1 Tat-Cyclophilin A-Capsid Protein Enables Tat Encapsidation That Is Required for HIV-1 Infectivity
Schatz M, Marty L, Ounadjela C, Tong PBV, Cardace I, Mettling C, Milhiet PE, Costa L, Godefroy C, Pugniere M, Guichou JF, Mesnard JM, Blaise M, Beaumelle B, J Virol, 2023
Structure and mechanics of the human nuclear pore complex basket using correlative AFM-fluorescence superresolution microscopy
Vial A, Costa L, Dosset P, Rosso P, Boutieres G, Faklaris O, Haschke H, Milhiet PE, Doucet CM, Nanoscale, 2023
The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity
Elena-Real CA, Sagar A, Urbanek A, Popovic M, Morato A, Estana A, Fournet A, Doucet C, Lund XL, Shi ZD, Costa L, Thureau A, Allemand F, Swenson RE, Milhiet PE, Crehuet R, Barducci A, Cortes J, Sinnaeve D, Sibille N, Bernado P, Nat Struct Mol Biol, 2023
2022
2021
CD82 and Gangliosides Tune CD81 Membrane Behavior
Fernandez L, Malrieu M, Benistant C, Dosset P, Rubinstein E, Odintsova E, Berditchevski F, Milhiet PE, Int J Mol Sci, 2021
Correlative AFM and fluorescence imaging demonstrate nanoscale membrane remodeling and ring-like and tubular structure formation by septins
Vial A, Taveneau C, Costa L, Chauvin B, Nasrallah H, Godefroy C, Dosset P, Isambert H, Ngo KX, Mangenot S, Levy D, Bertin A, Milhiet PE, Nanoscale, 2021
A Novel Approach to the Facile Growth and Organization of Photothermal Prussian Blue Nanocrystals on Different Surfaces
Ngo G, Felix G, Dorandeu C, Devoisselle JM, Costa L, Milhiet PE, Guari Y, Larionova J, Chopineau J, Nanomaterials (Basel), 2021
Mechanical Control of Cell Migration by the Metastasis Suppressor Tetraspanin CD82/KAI1
Ordas L, Costa L, Lozano A, Chevillard C, Calovoulos A, Kantar D, Fernandez L, Chauvin L, Dosset P, Doucet C, Heron-Milhavet L, Odintsova E, Berditchevski F, Milhiet PE, Benistant C, Cells, 2021
Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization
Bada Juarez JF, Judge PJ, Adam S, Axford D, Vinals J, Birch J, Kwan TOC, Hoi KK, Yen HY, Vial A, Milhiet PE, Robinson CV, Schapiro I, Moraes I, Watts A, Nat Commun, 2021
2020
TAT-RasGAP317-326 kills cells by targeting inner-leaflet-enriched phospholipids
Serulla M, Ichim G, Stojceski F, Grasso G, Afonin S, Heulot M, Schober T, Roth R, Godefroy C, Milhiet PE, Das K, Garcia-Saez AJ, Danani A, Widmann C, Proc Natl Acad Sci U S A, 2020
Author Correction: Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies
Fernandes TFD, Saavedra-Villanueva O, Margeat E, Milhiet PE, Costa L, Sci Rep, 2020
Compression, Rupture, and Puncture of Model Membranes at the Molecular Scale
Saavedra V O, Fernandes TFD, Milhiet PE, Costa L, Langmuir, 2020
Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies
Fernandes TFD, Saavedra O, Margeat E, Milhiet PE, Costa L, Sci Rep, 2020
2019
Nanoscale organization of tetraspanins during HIV-1 budding by correlative dSTORM/AFM
Dahmane S, Doucet C, Le Gall A, Chamontin C, Dosset P, Murcy F, Fernandez L, Salas D, Rubinstein E, Mougel M, Nollmann M, Milhiet PE, Nanoscale, 2019
Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy
Nasrallah H, Vial A, Pocholle N, Soulier J, Costa L, Godefroy C, Bourillot E, Lesniewska E, Milhiet PE, Methods Mol Biol, 2019
2018
2017
In-plane molecular organization of hydrated single lipid bilayers: DPPC:cholesterol
Gumi-Audenis B, Costa L, Redondo-Morata L, Milhiet PE, Sanz F, Felici R, Giannotti MI, Carla F, Nanoscale, 2017
Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation
Haining EJ, Matthews AL, Noy PJ, Romanska HM, Harris HJ, Pike J, Morowski M, Gavin RL, Yang J, Milhiet PE, Berditchevski F, Nieswandt B, Poulter NS, Watson SP, Tomlinson MG, Platelets, 2017
2016
Automatic detection of diffusion modes within biological membranes using back-propagation neural network
Dosset P, Rassam P, Fernandez L, Espenel C, Rubinstein E, Margeat E, Milhiet PE, BMC Bioinformatics, 2016
TOM1L1 drives membrane delivery of MT1-MMP to promote ERBB2-induced breast cancer cell invasion
Chevalier C, Collin G, Descamps S, Touaitahuata H, Simon V, Reymond N, Fernandez L, Milhiet PE, Georget V, Urbach S, Lasorsa L, Orsetti B, Boissiere-Michot F, Lopez-Crapez E, Theillet C, Roche S, Benistant C, Nat Commun, 2016
2015
TspanC8 tetraspanins differentially regulate the cleavage of ADAM10 substrates, Notch activation and ADAM10 membrane compartmentalization
Jouannet S, Saint-Pol J, Fernandez L, Nguyen V, Charrin S, Boucheix C, Brou C, Milhiet PE, Rubinstein E, Cell Mol Life Sci, 2015
HIV-1 nucleocapsid and ESCRT-component Tsg101 interplay prevents HIV from turning into a DNA-containing virus
Chamontin C, Rassam P, Ferrer M, Racine PJ, Neyret A, Laine S, Milhiet PE, Mougel M, Nucleic Acids Res, 2015
2014
Viruses and tetraspanins: lessons from single molecule approaches
Dahmane S, Rubinstein E, Milhiet PE, Viruses, 2014
Mimicking influenza virus fusion using supported lipid bilayers
Godefroy C, Dahmane S, Dosset P, Adam O, Nicolai MC, Ronzon F, Milhiet PE, Langmuir, 2014
Characterization of the structural modifications accompanying the loss of HBsAg particle immunogenicity
Greiner VJ, Manin C, Larquet E, Ikhelef N, Greco F, Naville S, Milhiet PE, Ronzon F, Klymchenko A, Mely Y, Vaccine, 2014
Milk sphingomyelin domains in biomimetic membranes and the role of cholesterol: morphology and nanomechanical properties investigated using AFM and force spectroscopy
Guyomarc'h F, Zou S, Chen M, Milhiet PE, Godefroy C, Vie V, Lopez C, Langmuir, 2014
2013
Hepatoma polarization limits CD81 and hepatitis C virus dynamics
Harris HJ, Clerte C, Farquhar MJ, Goodall M, Hu K, Rassam P, Dosset P, Wilson GK, Balfe P, Ijzendoorn SC, Milhiet PE, McKeating JA, Cell Microbiol, 2013
EWI-2wint promotes CD81 clustering that abrogates Hepatitis C Virus entry
Potel J, Rassam P, Montpellier C, Kaestner L, Werkmeister E, Tews BA, Couturier C, Popescu CI, Baumert TF, Rubinstein E, Dubuisson J, Milhiet PE, Cocquerel L, Cell Microbiol, 2013
Imaging of transmembrane proteins directly incorporated within supported lipid bilayers using atomic force microscopy
Levy D, Milhiet PE, Methods Mol Biol, 2013
Interaction of antibiotics with lipid vesicles on thin film porous silicon using reflectance interferometric Fourier transform spectroscopy
Guinan T, Godefroy C, Lautredou N, Pace S, Milhiet PE, Voelcker N, Cunin F, Langmuir, 2013
Structure and DNA-binding properties of the Bacillus subtilis SpoIIIE DNA translocase revealed by single-molecule and electron microscopies
Cattoni DI, Thakur S, Godefroy C, Le Gall A, Lai-Kee-Him J, Milhiet PE, Bron P, Nollmann M, Nucleic Acids Res, 2013
SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
Cattoni DI, Chara O, Godefroy C, Margeat E, Trigueros S, Milhiet PE, Nollmann M, EMBO Rep, 2013
2012
Self-assembling peptide-based nanoparticles for siRNA delivery in primary cell lines.
Deshayes S, Konate K, Rydstrom A, Crombez L, Godefroy C, Milhiet PE, Thomas A, Brasseur R, Aldrian G, Heitz F, Munoz-Morris MA, Devoisselle JM, Divita G, Small, 2012
Characterization of phospholipid bilayer formation on a thin film of porous SiO2 by reflective interferometric Fourier transform spectroscopy (RIFTS).
Pace S, Seantier B, Belamie E, Lautredou N, Sailor MJ, Milhiet PE, Cunin F, Langmuir, 2012
Atomic force microscopy: a versatile tool to probe the physical and chemical properties of supported membranes at the nanoscale.
Picas L, Milhiet PE, Hernandez-Borrell J, Chem. Phys. Lipids, 2012
2011
Nanoscale topography of hepatitis B antigen particles by atomic force microscopy
Milhiet PE, Dosset P, Godefroy C, Le Grimellec C, Guigner JM, Larquet E, Ronzon F, Manin C, Biochimie, 2011
Transfer on hydrophobic substrates and AFM imaging of membrane proteins reconstituted in planar lipid bilayers
Seantier B, Dezi M, Gubellini F, Berquand A, Godefroy C, Dosset P, Levy D, Milhiet PE, J Mol Recognit, 2011
2010
Preferential insertion of lactose permease in phospholipid domains: AFM observations
Picas L, Carretero-Genevrier A, Montero MT, Vazquez-Ibar JL, Seantier B, Milhiet PE, Hernandez-Borrell J, Biochim Biophys Acta, 2010
A Grafting of monoglyceride molecules for the design of hydrophilic and stable porous silicon surfaces
Pace S, Gonzalez P, Devoisselle J, Milhiet P, Brunel D, Cunin F, N.J. Chem, 2010
Deciphering the structure, growth and assembly of amyloid-like fibrils using high-speed atomic force microscopy
Milhiet PE, Yamamoto D, Berthoumieu O, Dosset P, Le Grimellec C, Verdier JM, Marchal S, Ando T, PLoS One, 2010
Surface topography of membrane domains
Giocondi MC, Yamamoto D, Lesniewska E, Milhiet PE, Ando T, Le Grimellec C, Biochim Biophys Acta, 2010
HIV-1 assembly differentially alters dynamics and partitioning of tetraspanins and raft components
Krementsov DN, Rassam P, Margeat E, Roy NH, Schneider-Schaulies J, Milhiet PE, Thali M, Traffic, 2010
The gelsolin:calponin complex nucleates actin filaments with distinct morphologies
Ferjani I, Fattoum A, Bettache N, Seantier B, Milhiet PE, Manai M, Benyamin Y, Roustan C, Maciver SK, Biochem Biophys Res Commun, 2010
Organization of influenza A virus envelope at neutral and low pH
Giocondi MC, Ronzon F, Nicolai MC, Dosset P, Milhiet PE, Chevalier M, Le Grimellec C, J Gen Virol, 2010
2009
Lateral organization of membrane proteins: tetraspanins spin their web
Charrin S, le Naour F, Silvie O, Milhiet PE, Boucheix C, Rubinstein E, Biochem J, 2009
Calcium-induced formation of subdomains in phosphatidylethanolamine-phosphatidylglycerol bilayers: a combined DSC, 31P NMR, and AFM study
Picas L, Montero MT, Morros A, Cabanas ME, Seantier B, Milhiet PE, Hernandez-Borrell J, J Phys Chem B, 2009
2008
Temperature-dependent imaging of living cells by AFM
Espenel C, Giocondi MC, Seantier B, Dosset P, Milhiet PE, Le Grimellec C, Ultramicroscopy, 2008
Single-molecule analysis of CD9 dynamics and partitioning reveals multiple modes of interaction in the tetraspanin web
Espenel C, Margeat E, Dosset P, Arduise C, Le Grimellec C, Royer CA, Boucheix C, Rubinstein E, Milhiet PE, J Cell Biol, 2008
Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM
Giocondi MC, Seantier B, Dosset P, Milhiet PE, Le Grimellec C, Pflugers Arch, 2008
Inhibition of the mitogenic, angiogenic and tumorigenic activities of pleiotrophin by a synthetic peptide corresponding to its C-thrombospondin repeat-I domain
Hamma-Kourbali Y, Bernard-Pierrot I, Heroult M, Dalle S, Caruelle D, Milhiet PE, Fernig DG, Delbe J, Courty J, J Cell Physiol, 2008
Probing supporting model and native membranes using afm
Seantier B, Giocondi MC, Le Grimellec C, Milhiet PE, Current Opinion in Colloid and Interface Science, 2008
2007
Continuous planar phospholipid bilayer supported on porous silicon thin film reflector
Cunin F, Milhiet PE, Anglin E, Sailor MJ, Espenel C, Le Grimellec C, Brunel D, Devoisselle JM, Ultramicroscopy, 2007
Nanoforce et Imagerie AFM
Le Grimellec C, Milhiet PE, Perez E, Pincet F, Aime JP, Emiliani V, Thoumine O, Lionnet T, Croquette V, Allemand JF, Bensimon D, Les Nanosciences: Nanobiotechnologies et nanobiologie, 2007
Remodeling of ordered membrane domains by GPI-anchored intestinal alkaline phosphatase
Giocondi MC, Besson F, Dosset P, Milhiet PE, Le Grimellec C, Langmuir, 2007
Temperature-dependent localization of GPI-anchored intestinal alkaline phosphatase in model rafts
Giocondi MC, Besson F, Dosset P, Milhiet PE, Le Grimellec C, J Mol Recognit, 2007
Influence of calcium on direct incorporation of membrane proteins into in-plane lipid bilayer
Berquand A, Levy D, Gubellini F, Le Grimellec C, Milhiet PE, Ultramicroscopy, 2007
Glycosylation status of the membrane protein CD9P-1
Andre M, Morelle W, Planchon S, Milhiet PE, Rubinstein E, Mollicone R, Chamot-Rooke J, Le Naour F, Proteomics, 2007
2006
2005
2004
Heparin affin regulatory peptide in milk: its involvement in mammary gland homeostasis
Bernard-Pierrot I, Delbe J, Heroult M, Rosty C, Soulie P, Barritault D, Milhiet PE, Courty J, Biochem Biophys Res Commun, 2004
Calcitonin-derived carrier peptide plays a major role in the membrane localization of a peptide-cargo complex
Boichot S, Krauss U, Plenat T, Rennert R, Milhiet PE, Beck-Sickinger A, Le Grimellec C, FEBS Lett, 2004
Use of cyclodextrin for AFM monitoring of model raft formation
Giocondi MC, Milhiet PE, Dosset P, Le Grimellec C, Biophys J, 2004
Interaction of primary amphipathic cell-penetrating peptides with phospholipid-supported monolayers
Plenat T, Deshayes S, Boichot S, Milhiet PE, Cole RB, Heitz F, Le Grimellec C, Langmuir, 2004
2003
AFM imaging of lipid domains in model membranes
Milhiet PE, Giocondi MC, Le Grimellec C, ScientificWorldJournal, 2003
From cell imaging to molecular manipulation: biological applications of atomic force microscopy
Giocondi MC, Milhiet PE, Lesniewska E, Le Grimellec C, M S Medecine Sciences, 2003
2002
Dominant negative effectors of heparin affin regulatory peptide (HARP) angiogenic and transforming activities
Bernard-Pierrot I, Delbe J, Rouet V, Vigny M, Kerros ME, Caruelle D, Raulais D, Barritault D, Courty J, Milhiet PE, J Biol Chem, 2002
Fragments peptidiques du facteur de croissance HARP inhibant l'angiogenese
Courty J, Milhiet PE, Bernard-Pierrot I, Delbe J, Barritault D, , 2002
Atomic force microscope imaging of cells and membranes
Lesniewska E, Milhiet PE, Giocondi MC, Le Grimellec C, Methods Cell Biol, 2002
AFM detection of GPI protein insertion into DOPC/DPPC model membranes
Milhiet PE, Giocondi MC, Baghdadi O, Ronzon F, Le Grimellec C, Roux B, Single Mol., 2002
Spontaneous insertion and partitioning of alkaline phosphatase into model lipid rafts
Milhiet PE, Giocondi MC, Baghdadi O, Ronzon F, Roux B, Le Grimellec C, EMBO Rep, 2002
Cholesterol is not crucial for the existence of microdomains in kidney brush-border membrane models
Milhiet PE, Giocondi MC, Le Grimellec C, J Biol Chem, 2002
Immunoassay for measuring the heparin-binding growth factors HARP and MK in biological fluids
Soulie P, Heroult M, Bernard I, Kerros ME, Milhiet PE, Delbe J, Barritault D, Caruelle D, Courty J, J Immunoassay Immunochem, 2002
2001
AFM characterization of model rafts in supported bilayers
Milhiet PE, Vie V, Giocondi MC, Le Grimellec C, Single Mol., 2001
Domain formation in models of the renal brush border membrane outer leaflet
Milhiet PE, Domec C, Giocondi MC, Van Mau N, Heitz F, Le Grimellec C, Biophys J, 2001
Phase topology and growth of single domains in lipid bilayers
Giocondi MC, Vie V, Lesniewska E, Milhiet PE, Zinke-Allmang M, Le Grimellec C, Langmuir, 2001
Temperature dependence of the topology of supported dimirystoyl- distearoyl phosphatidylcholine bilayers
Giocondi MC, Pacheco L, Milhiet PE, Le Grimellec C, Ultramicroscopy, 2001
The lysine-rich C-terminal tail of heparin affin regulatory peptide is required for mitogenic and tumor formation activities
Bernard-Pierrot I, Delbe J, Caruelle D, Barritault D, Courty J, Milhiet PE, J Biol Chem, 2001
2000
1999
Glycosaminoglycans promote HARP/PTN dimerization
Bernard-Pierrot I, Heroult M, Lemaitre G, Barritault D, Courty J, Milhiet PE, Biochem Biophys Res Commun, 1999
[Implication of HARP in angiogenesis and possible therapeutic role]
Delbe J, Katsoris P, Milhiet PE, Barritault D, Caruelle D, Courty J, Pathol Biol (Paris), 1999
1998
1995
1994
Expression of rat endopeptidase-24.18 in COS-1 cells: membrane topology and activity
Milhiet PE, Corbeil D, Simon V, Kenny AJ, Crine P, Boileau G, Biochem J, 1994
Fate of a fluorescent inhibitor of endopeptidase-24.11 using enzyme- expressing MDCK cells. Modification of its cellular processing with a monoclonal antibody
Milhiet PE, Giocondi MC, Crine P, Le Grimellec C, Roques BP, Garbay-Jaureguiberry C, Eur J Cell Biol, 1994
1993
Rat endopeptidase-24.18 alpha subunit is secreted into the culture medium as a zymogen when expressed by COS-1 cells
Corbeil D, Milhiet PE, Simon V, Ingram J, Kenny AJ, Boileau G, Crine P, FEBS Lett, 1993
Complete sequence of rabbit kidney aminopeptidase N and mRNA localization in rabbit kidney by in situ hybridization
Yang XF, Milhiet PE, Gaudoux F, Crine P, Boileau G, Biochem Cell Biol, 1993
1992
Effects of monoclonal antibodies raised against the common acute lymphoblastic leukemia antigen on endopeptidase-24.11 activity
Helene A, Milhiet PE, Haouas H, Boucheix C, Beaumont A, Roques BP, Biochem Pharmacol, 1992
Increase of neutral endopeptidase-24.11 with cellular density and enzyme modulation with an inhibitor on human Reh6 cell line
Milhiet PE, Beaumont A, Garbay-Jaureguiberry C, Roques BP, Biochem Pharmacol, 1992
Detection of neutral endopeptidase-24.11/CD10 by flow cytometry and photomicroscopy using a new fluorescent inhibitor
Milhiet PE, Dennin F, Giocondi MC, Le Grimellec C, Garbay-Jaureguiberry C, Boucheix C, Roques BP, Anal Biochem, 1992