Study of interaction kinetics and enzymatic pathways
Determination of the transient kinetics of protein-ligand interactions and of enzymatic processes
Transient kinetics techniques (stopped-flow, quench-flow, cryoenzymology) are used to measure enzymatic kinetics on the millisecond time scale. The main applications of these techniques are :
Example of transient kinetics obtained with quench-flow and relationship between the transient phases and different steps of the reaction pathway.