Vitrobot (ThermoFischer)

Equipement

Vitrobot (ThermoFischer)

Frozen hydrated sample preparation

 

 

 
Room Z3-9@CBS29

Pumping Station for cryo holders

Equipement

Pumping Station (Gatan) for cryo holders

Measure

Structural analysis of frozen hydrated single particles, Cryo electron tomography, micro electron diffraction

For cryo holders

 

 

 
Room Z3-9@CBS29

Jeol 1400

Equipement

Cryo Electron Microscope

Measure

Sample analysis in negative stain, electron tomography or frozen hydrated sample

EM to analyse singles particles samples in negative stain,1400 frozen hydrated sample, or cells by electron tomography

  Microscope JEOL 1400

 JEOL 1400 Flash transmission electron microscope

 
Specifications

It is a LaB6 microscope working at 120kV, equipped with a GATAN One View camera to record images. This microscope is open to every user after training, to screen and to check their sample quality, then, have a 2D classes after acquisition on negative stained samples. This microscope is also equipped with cryo pole piece to allow frozen hydrated samples observation. The implemented Shot Meister software allows low dose acquisition, suitable for biological samples that are highly sensitive to electron damages. For cryo electron tomography, Serial EM software is used to collect tilted series from -60° to +60°.

https://www.jeolusa.com/PRODUCTS/Transmission-Electron-Microscopes-TEM

Room Z3-4@CBS29

Jeol 2200FS Cryo Electron Microscope

Equipement

Cryo Electron Microscope

Measure

Structural analysis of frozen hydrated single particles, Cryo electron tomography, micro electron diffraction

CryoEM to analyse frozen hydrated single particles samples, or by electron tomography or electron micro diffraction

 

JEOL 2200FS transmission electron microscope

 
Specifications

It is a FEG microscope working at 200kV, equipped with a GATAN direct detector K3 camera (installed in 2021) to record images. This microscope have a cryo pole piece to allow frozen hydrated samples observation, it is also have a in-column energy filter (Omega filter). The implemented iMDS software allows low dose acquisition, suitable for biological samples that are highly sensitive to electron damages. For cryo electron tomography, Serial EM software is used to collect tilted series from -60° to +60°. For electron micro diffraction, the ASI Cheetah camera (1M Pixels) will be installed in the near future.

https://www.jeolusa.com/PRODUCTS/Transmission-Electron-Microscopes-TEM

Room Z3-9@CBS29

Connexion