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Measuring molecule diffusions

We propose various approaches (FCS, FCCS, N&B, SPT and SPT-PALM) to measure the diffusion characteristics of fluorescent molecules, in vitro or in live cells. Depending on the approach, we can access the oligomerization state, the local concentration, the diffusion constants of one or several fluorescent populations, the directionality...
 

PALM

sptPALM in A.thaliana - credit: Alexandre Martinière

 

All projects are run as collaborations. Please This email address is being protected from spambots. You need JavaScript enabled to view it. to help you choose the most appropriate approach. We also provide advice for the design of your experiment and support for data analysis. After a meeting, we can conduct some experiments to assess the feasibility of the approach. Then a collaboration scheme is defined between the team and the facility. Our R&D division can also implement specific adaptations for your project if needed.

Selected publications:

B. Guilhas, J.C. Walter, J. Rech, G. David, N.-O. Walliser, J. Palmeri, C., Mathieu-Demaziere, A. Parmeggiani, J.Y. Bouet, A. Le Gall, M. Nollmann. ATP-driven separation of liquid phase condensates in bacteria, Molecular Cell, 2020 Jul 16;79(2):293-303.e4. pubmed Id 32679076

Martiniere A, Fiche JB, Smokvarska M, Mari S, Alcon C, Dumont X, Hematy K, Jaillais Y, Nollmann M, Maurel C Osmotic stress activates two reactive oxygen species pathways with distinct effects on protein nanodomains and diffusion Plant Physiol 2019, 179 ( 4): 1581-1593 pubmed Id 30718348

Platre MP, Bayle V, Armengot L, Bareille J, Marques-Bueno MDM, Creff A, Maneta-Peyret L, Fiche JB, Nollmann M, Miege C, Moreau P, Martiniere A, Jaillais Y Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine Science 2019,364(6435):57-62 pubmed Id 30948546

Faure LM, Fiche JB, Espinosa L, Ducret A, Anantharaman V, Luciano J, Lhospice S, Islam ST, Treguier J, Sotes M, Kuru E, Van Nieuwenhze MS, Brun YV, Theodoly O, L A, Nollmann M, Mignot T The mechanism of force transmission at bacterial focal adhesion complexes Nature 2016, 539(7630):530-535 pubmed Id 27749817

​Antoine Le Gall, Diego I. Cattoni, Baptiste Guilhas, Céline Mathieu-Demazière, Laura Oudjedi, Jean-Bernard Fiche, Jérôme Rech, Sara Abrahamsson, Heath Murray, Jean-Yves Bouet, Marcelo Nollmann. Bacterial partition complexes segregate within the volume of the nucleoid. Nature Communications, 7: 12107, 5 July 2016

Dosset P, Rassam P, Fernandez L, Espenel C, Rubinstein E, Margeat E, Milhiet PE. Automatic detection of diffusion modes within biological membranes using back-propagation neural network, BMC Bioinformatics, 2016

Equipement

 

2 photons FCS

 

SmFRET TCSPC

 

TIRF / 3D MFM

 

2 photons STED / FCS

FCS
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  smFRET
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FCS
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PALM

           
FCS
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Connexion