Single Molecule Localization Microscope
PALM, dSTORM, SPT-PALM, TIRF
|Specifications||Custom-made inverted microscope (Zeiss stage)
objective: Plan-Apocromat, 100x, 1.4NA oil DIC (Zeiss)
z-axis piezoelectric stage (P-721.CDQ, PICOF, PI).
1.5x telescope (pixel size ≈105 nm)
Emission filters: ET525/50 m, ET600/50 m and ET700/75m (Chroma Technology)
EMCCD camera (iXon X3 DU-897, Andor Technologies)
Sample motorized stage
Home-made auto- focus
Camera, lasers and filter wheel are controlled with software written in Labview.
|Comment||For full description, please refer to: Georgieva et al, Methods (2016)