Plateforme Intégrée de Biophysique et de Biologie Structurale

Equipement

2 photons STED / FCS

Managers Caroline Clerté , Emmanuel Margeat, Marcelo Nollmann

FCS, FCCS, N&B, RICS…
3D STED, fastFLIM, STED FCS
possibility to do FCCS without crosstalk (PIE)

 
2 photons STED-FCS
 
Specifications Pulsed laser Mai Tai 680-1050nm
ISS laser scanning module with 2APD + 2PMT
Detection of classical dyes (CFP, GFP, mCherry, Cy5).
Epi-illumination lamp
Nikon statif:
  • 60X NA 1.4 oil
  • 60X NA 1.2 water (=> deeper in the sample + less chromatic aberrations)
z-piezo on the objective + used to be an autofocus, should be reinstalled.
Sample motorized stage (MS-2000, ASI)
Fast FLIM module
4 Picoquant lasers (405, 485, 595, 640nm) + combiner
Picoquant SepiaII controller => individual laser control for PIE, slaved to the Mai Tai master clock
Shape of the STED laser by an SLM (double reflection on the SLM => lateral then axial PSF engineering).
Comment This machine is currently under construction
Room @MARS

Connexion