A protocol for the incorporation of selenomethionine and selenocysteine into recombinant proteins overexpressed in Escherichia coli based on the use of a suitable cysteine auxotrophic strain.

Strub MP., Hoh F., Sanchez JF., Strub JM., Böck A., Aumelas A. & Dumas C. (2003) "Selenomethionine and Selenocysteine Double Labeling Strategy for Crystallographic Phasing", Structure , 11, 1359-1367.