PALM

Equipement

PALM

Measure

Single Molecule Localization Microscope

PALM, dSTORM, SPT-PALM, TIRF
 
 
Specifications Custom-made inverted microscope (Zeiss stage)
objective: Plan-Apocromat, 100x, 1.4NA oil DIC (Zeiss)
z-axis piezoelectric stage (P-721.CDQ, PICOF, PI).
1.5x telescope (pixel size ≈105 nm)
Lasers:
  • 405 nm (OBIS, LX 405-50, Coherent Inc.)
  • 488 nm (OBIS, LX 488-50, Coherent Inc.)
  • 561 nm (Sapphire)
  • 640 nm (OBIS, LX 640-100, Coherent Inc.)
AOTF: AOTFnc-400.650-TN, AA (opto-electronics)
Emission filters: ET525/50 m, ET600/50 m and ET700/75m (Chroma Technology)
EMCCD camera (iXon X3 DU-897, Andor Technologies)
Sample motorized stage
Home-made auto- focus
Camera, lasers and filter wheel are controlled with software written in Labview.
Comment For full description, please refer to: Georgieva et al, Methods (2016)
https://www.sciencedirect.com/science/article/pii/S1046202316300639
Room @MARS

STED FCS

Equipement

2 photons STED / FCS

FCS, FCCS, N&B, RICS…
3D STED, fastFLIM, STED FCS
possibility to do FCCS without crosstalk (PIE)
 
2 photons STED-FCS		  
Specifications Pulsed laser Mai Tai 680-1050nm
ISS laser scanning module with 2APD + 2PMT
Detection of classical dyes (CFP, GFP, mCherry, Cy5).
Epi-illumination lamp
Nikon statif:
  • 60X NA 1.4 oil
  • 60X NA 1.2 water (=> deeper in the sample + less chromatic aberrations)
z-piezo on the objective + used to be an autofocus, should be reinstalled.
Sample motorized stage (MS-2000, ASI)
Fast FLIM module
4 Picoquant lasers (405, 485, 595, 640nm) + combiner
Picoquant SepiaII controller => individual laser control for PIE, slaved to the Mai Tai master clock
Shape of the STED laser by an SLM (double reflection on the SLM => lateral then axial PSF engineering).
Comment This machine is currently under construction
Room @MARS

Connexion