technique

Automated crystallisation

*Service*	Crystallisation screening and crystallisation conditions optimization
*Goal*	Obtaining diffracting protein crystals which are required to obtain atomic-level structures via X-ray diffraction techniques
The crystallization experiments are set up in 96 well sitting drop plates via nano-dispensing robot (50-100 nl/drop, Mosquito, STPLabtech). For initial screening we're using commercial screens and for crystallization optimization, commercial additifs and home made solution . The facility is equipped with an automated imaging system for protein crystallization (Rock Imager, Formulatrix).
	Used plates: 96 well plates and 24 wells plates

Equipement

Mosquito HTS (Stplabtech) nano dispensing	Dragongly (StpLabtech) crystallisation conditions optimization
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Ligand screening

Service

Ligand screening

Goal

Drug design

Libraries of small molecules are screened by soaking onto protein crystals or (dry) co-crytallization. Python scripts allows a fast treatment of large number of diffraction data.

screening and conception for CypD

Equipement

Automated visualization of protein crystallization drops

Service

Automated visualization of protein crystallization drops

Goal

Automated imaging system of crystallization drops

Protein Crystallization Imagers capture images of protein drops and provide critical information about crystals. Images are accessible to users and can be scored via a web-interface.

Equipement

*Service*	Automated pippeting and liquid handling
*Goal*	Automated plate filling
SBS plates filling, 96 and 8 tips liquid handling.

Automated crystallisation

Crystallisation screening and crystallisation conditions optimization

Mosquito HTS (Stplabtech) nano dispensing

Dragongly (StpLabtech) crystallisation conditions optimization

Automated pippeting and liquid handling

Automated pippeting and liquid handling

OT2 Opentrons robot

Cold chamber

Ligand screening

Ligand screening

OT2 Opentrons robot

Mosquito HTS (Stplabtech) nano dispensing

Rock Imager R1000 (Formulatrix)

Automated visualization of protein crystallization drops

Automated visualization of protein crystallization drops

Rock Imager R1000 (Formulatrix)

Connexion