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| Service |
Spectral Assignments
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| Goal |
Characterize macromolecule samples by 1D, 2D NMR, recording of 2D, 3D, nD NMR experiments and assignments. |
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The sample quality is checked by 1D/2D NMR, then 3D or nD NMR experiments are recorded, and spectra are assigned. |
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| Equipement |
800 MHz NMR spectrometer
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700 MHz NMR spectrometer
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600 MHz NMR spectrometer
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| Service |
Structure Determination
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| Goal |
Recording of 2D/3D NOESY NMR spectra, automatic assignments using Cyana 3 and refinement. |
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The structure is calculated using unassigned NOESY peak lists, assigned chemical shifts list and dihedral constraints. The preliminary structure is refined step-by-step. |
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| Service |
HP NMR and screening
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| Goal |
Folding pathway of proteins at the residue level. Ligand screening at low throughput. |
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Series of HSQC spectra are recorded at increasing hydrostatic pressure.
Thermodynamic analysis allows characterizing the unfolding/folding pathway.
Output : Series of thermodynamic parameters and intermediate structures. |
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| Equipement |
700 MHz NMR spectrometer
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600 MHz NMR spectrometer
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