Bruker multimode III

Equipement

Multimode equipped with Nanoscope III (BRUKER)

Measure

Characterize biological samples topography at nanoscale in dry or liquid environment. Image acquisition can be performed in static or dynamic modes.

The technique allows characterizing sample morphology at nanoscale. Images are acquired by scanning a nanometric tip in gentle contact or intermittent contact with the sample. The tip is positioned at the end of a micrometric force transducer, the AFM cantilever: it records the variation of sample topography due to changes of tip-sample interaction during scanning operation. In addition, by measuring the tip-sample interaction force as a function of the tip-sample distance, AFMs can evaluate sample elastic and viscous properties.

 
Bruker Multimode Nanoscope III
 
Room 0B13@CBS60

Bruker multimode VIII

Equipement

Multimode equipped with Nanoscope VIII (BRUKER)

Measure

Characterize biological samples topography at nanoscale in dry or liquid environment. Image acquisition can be performed in static or dynamic modes.

The technique allows characterizing sample morphology at nanoscale. Images are acquired by scanning a nanometric tip in gentle contact or intermittent contact with the sample. The tip is positioned at the end of a micrometric force transducer, the AFM cantilever: it records the variation of sample topography due to changes of tip-sample interaction during scanning operation. In addition, by measuring the tip-sample interaction force as a function of the tip-sample distance, AFMs can evaluate sample elastic and viscous properties.

 
INSTRUMENT-BRUKER-MULTIMODEVIII.jpgBruker Multimode Nanoscope VIII
 
Specifications Equipped with Peak-Force Tapping option.
Room 1A10@CBS60

JPK nanowizard4 confocal

Equipement

Correlative AFM with Fluorescence Spectroscopy

Measure

Correlate biomolecules activity observed by Fluorescence Spectroscopy (FCS, PIE-FRET and FLIM) with the associated sample topology and mechanics observed by AFM.

Our Confocal-AFM correlative setup is mounted on top of a Zeiss inverted microscope. Fast AFM imaging (1 frame in few seconds) can be performed simultaneously with the confocal fluorescence microscopy operation. Two avalanche photodetectors (APDs) ensure two colors imaging and spectroscopy capabilities. Designed in a Pulsed Interleaved Excitation (PIE-FRET) operational scheme.

 
JPK Nanowizard 4 coupled to Super Resolution Fluorescence Microscopy and TIRF
 
Specifications AFM:
  • X - Y scan (Tip scanning): 100 micron each
  • Z scan: 15 micron (Head) or 10 micron (Sample, TAO stage)
  • Equipped with the ultra-High-Speed cantilever holder that can achieve a scan speed of 1 image/second, a petri dish heater for living cells experiments at 37 °C and with the Advanced Quantitative-Imaging (QI) option.
FLUORESCENCE:
  • Supercontinuum laser (Leukos) that permits to select any visibile excitation laser wavelength
  • Oil immersion objective from Zeiss, 1.4 NA, 100X
  • Two APDs: a PicoQuant and a PerkinHelmer for two colors imaging and spectroscopy.
Room Z3-7@CBS29

HS AFM

Equipement

High-Speed AFM

Measure

Real-time visualization of biomolecules activity at high spatial-temporal resolution

Our HS-AFM can acquire AFM images up to 10 images/seconds, allowing for dynamic tracking of slow biomolecules activity.

 
High-Speed Atomic Force Microscope (Developed in collaboration with Pr.Toshio Ando)
 
Specifications For technical info about HS-AFM, please read https://doi.org/10.1088/0957-4484/23/6/062001
Room 1A11@CBS60

JPK nanowizard4 SR

Equipement

Correlative AFM with Super-Resolution Fluorescence, TIRF and EPI

Measure

Correlate biomolecules activity observed by Fluorescence (Epi and TIRF) or Super Resolution Microscopy (PALM/STORM) with the associated sample topology observed by AFM.

Our fluorescence-AFM correlative setup is mounted on top of a Zeiss inverted microscope. Fast AFM imaging (1 frame in few seconds) can be performed simultaneously with the fluorescence microscopy operation.

 
JPK Nanowizard 4 coupled to Super Resolution Fluorescence Microscopy and TIRF
 
Specifications AFM:
  • X - Y scan (Tip scanning): 100 micron each
  • Z scan: 15 micron (Head) or 100 micron (Sample, CellHesion)
  • Equipped with the ultra-High-Speed cantilever holder that can achieve a scan speed of 1 image/second, a petri dish heater for living cells experiments at 37 °C and with the Advanced Quantitative-Imaging (QI) option.
FLUORESCENCE:
  • Four laser lines: 405 nm, 488 nm, 560 nm and 640 nm (Coherent)
  • Oil immersion objective from Zeiss, 1.45 NA, 100X.
  • iXon EMCCD from Andor that can be cooled down by liquid flow to ensure minimal invasiveness between EMCCD vibrations and the AFM stability.
Room 1A10@CBS60

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  1. X-AFM

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