Plateforme Intégrée de Biophysique et de Biologie Structurale

Equipement

PALM

Manager Jean-Bernard Fiche
Measure

Single Molecule Localization Microscope

PALM, dSTORM, SPT-PALM, TIRF

 
 
Specifications Custom-made inverted microscope (Zeiss stage)
objective: Plan-Apocromat, 100x, 1.4NA oil DIC (Zeiss)
z-axis piezoelectric stage (P-721.CDQ, PICOF, PI).
1.5x telescope (pixel size ≈105 nm)
Lasers:
  • 405 nm (OBIS, LX 405-50, Coherent Inc.)
  • 488 nm (OBIS, LX 488-50, Coherent Inc.)
  • 561 nm (Sapphire)
  • 640 nm (OBIS, LX 640-100, Coherent Inc.)
AOTF: AOTFnc-400.650-TN, AA (opto-electronics)
Emission filters: ET525/50 m, ET600/50 m and ET700/75m (Chroma Technology)
EMCCD camera (iXon X3 DU-897, Andor Technologies)
Sample motorized stage
Home-made auto- focus
Camera, lasers and filter wheel are controlled with software written in Labview.
Comment For full description, please refer to: Georgieva et al, Methods (2016)
https://www.sciencedirect.com/science/article/pii/S1046202316300639
Room @MARS

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