Correlative AFM with Fluorescence Spectroscopy
Correlate biomolecules activity observed by Fluorescence Spectroscopy (FCS, PIE-FRET and FLIM) with the associated sample topology and mechanics observed by AFM.
Our Confocal-AFM correlative setup is mounted on top of a Zeiss inverted microscope. Fast AFM imaging (1 frame in few seconds) can be performed simultaneously with the confocal fluorescence microscopy operation. Two avalanche photodetectors (APDs) ensure two colors imaging and spectroscopy capabilities. Designed in a Pulsed Interleaved Excitation (PIE-FRET) operational scheme.
JPK Nanowizard 4 coupled to Super Resolution Fluorescence Microscopy and TIRF