Plateforme Intégrée de Biophysique et de Biologie Structurale

Equipement

2 photons FCS

Manager Caroline Clerté
Measure

Measure diffusion, concentration, brightness or oligomerization state of fluorescently labeled molecules in vitro or in vivo.

FCS, FCCS, Number and Brightness, RICS…
Simultaneous acquisition in 2 emission channels

 
2 photons FCS @CBS29
 
Specifications
  • Mai Tai Laser 700-1020nm. 2 photons excitation
  • ISS fast laser scanning module
  • Detection on 2 low dark counts APDs with 5 different dichroic and filters sets to detect fluorescence from classical dyes (CFP, GFP, mCherry, Cy5…).
  • Epi-illumination lamp
  • Nikon statif + 60X NA 1.4 oil
60X NA 1.2 water (=> deeper in the sample + less chromatic aberrations)
  • Z-piezo on the objective
  • No auto-focus system.
  • Different sample holders
  • No heating system, no CO2 control.
Room Z2-17@CBS29

Connexion