Plateforme Intégrée de Biophysique et de Biologie Structurale

Service

Micro electron diffraction

Goal

Structural analysis of tiny crystals by electron diffraction

Micro electron diffraction is a recently developed method in cryo electron microscopy that allow the collection of high resolution electron diffraction data from extremely small three-dimensional crystals that are in the range of 0,1 -0,4 mm thick using a transmission electron microscope. We implemented this approach on the JEOL 2200FS electron microscope and investigated its application to membrane protein crystals.

 


Electron diffraction pattern of Lysozyme micro-crystal and its computed structure

 
Comment Need to prepare crystals (see X-ray crystals preparation) then cryo EM sample freezing.
Equipement

Cryo Electron Microscope

 

Vitrobot (ThermoFischer)

 

Pumping Station (Gatan) for cryo holders

       

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Connexion