Plateforme Intégrée de Biophysique et de Biologie Structurale

Service

AFM imaging at high resolution in liquid

Goal

Characterize biological samples topography at nanoscale in dry or liquid environment. Image acquisition can be performed in static or dynamic modes

The technique allows characterizing sample morphology at nanoscale. Images are acquired by scanning a nanometric tip in gentle contact or intermittent contact with the sample. The tip is positioned at the end of a micrometric force transducer, the AFM cantilever: it records the variation of sample topography due to changes of tip-sample interaction during scanning operation. In addition, by measuring the tip-sample interaction force as a function of the tip-sample distance, AFMs can evaluate sample elastic and viscous properties.

 


Cholera Toxin B-oligomers (left) bound to GM1 domains within a DOPC-DPPC (1:1) model membrane (right) as observed by AFM. Milhiet, Pierre Emmanuel, et al. "AFM characterization of model rafts in supported bilayers." Single molecules 2.2 (2001): 109-112

 
Equipement

Correlative AFM with Super-Resolution Fluorescence, TIRF and EPI

 

Correlative AFM with Fluorescence Spectroscopy

 

High-Speed Atomic Force Microscopy

 

Multimode equipped with Nanoscope VIII (BRUKER)


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  INSTRUMENT-HS-AFM
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Multimode equipped with Nanoscope III (BRUKER)

 

X-AFM: AFM that can be installed in Synchrotron X-Ray beamlines.

       

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Connexion